Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,346,770 G→C 6.1% intergenic (‑28/‑26) gmr ← / → ymiB cyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,346,7700GC 6.1% 233.0 / 5.7 66intergenic (‑28/‑26)gmr/ymiBcyclic‑di‑GMP phosphodiesterase; csgD regulator; modulator of RNase II stability/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (28/34);  new base C (2/2);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

GGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTA  >  NC_000913/1346624‑1346849
                                                                                                                                                  |                                                                               
ggTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGt                                                                                <  3:948358/148‑1 (MQ=255)
       tgGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCgg                                                                          >  3:617486/1‑147 (MQ=255)
       tgGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCgg                                                                          <  4:617486/147‑1 (MQ=255)
           ttCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaa                                                                     >  4:555437/1‑148 (MQ=255)
               gtggtAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 <  1:394989/148‑1 (MQ=255)
                      aaaaGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 <  1:299217/141‑1 (MQ=255)
                      aaaaGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 >  2:299217/1‑141 (MQ=255)
                        aaGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 <  3:293822/139‑1 (MQ=255)
                        aaGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 >  4:293822/1‑139 (MQ=255)
                             cAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaa                                                                     <  1:486943/130‑1 (MQ=255)
                             cAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaa                                                                     >  2:486943/1‑130 (MQ=255)
                              aaaaCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACt                                                  <  4:793104/148‑1 (MQ=255)
                                aaCATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTa                                                <  1:184495/148‑1 (MQ=255)
                                  cATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACa                                              <  2:770642/148‑1 (MQ=255)
                                  cATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACa                                              <  2:48138/148‑1 (MQ=255)
                                     cGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACCGCGGACTCcgctg                                                                          >  1:881946/1‑115 (MQ=255)
                                     cGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACCGCGGACTCcgctg                                                                          <  2:881946/117‑3 (MQ=255)
                                      gctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGaaca                                                          >  1:989849/1‑132 (MQ=255)
                                      gctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGaaca                                                          <  2:989849/132‑1 (MQ=255)
                                       ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGt                                                                         >  4:1137792/1‑116 (MQ=255)
                                       ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGt                                                                         <  3:1137792/116‑1 (MQ=255)
                                       ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGa                                                             <  1:548162/128‑1 (MQ=255)
                                       ctgctTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGa                                                             >  2:548162/1‑128 (MQ=255)
                                            ttCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTaa                                     <  1:860303/147‑1 (MQ=255)
                                            ttCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTaa                                     >  2:860303/1‑147 (MQ=255)
                                                         cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCcg                                                                             <  1:833861/94‑1 (MQ=255)
                                                         cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCcg                                                                             >  2:833861/1‑94 (MQ=255)
                                                         cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGtt                                                                        <  4:1113215/99‑1 (MQ=255)
                                                         cAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGtt                                                                        >  3:1113215/1‑99 (MQ=255)
                                                                    gtgCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGttttttt            <  3:555437/148‑1 (MQ=255)
                                                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 <  4:245689/90‑1 (MQ=255)
                                                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaaataaa                                                                 >  3:245689/1‑90 (MQ=255)
                                                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAt                     <  4:764826/134‑1 (MQ=255)
                                                                         aTCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAt                     >  3:764826/1‑134 (MQ=255)
                                                                              agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACCGCGGACTCCGCTGTta                                                                       <  4:1122057/79‑1 (MQ=38)
                                                                              agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACCGCGGACTCCGCTGTta                                                                       >  3:1122057/1‑79 (MQ=39)
                                                                              agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTta          <  1:1086797/140‑1 (MQ=255)
                                                                              agaAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTta          >  2:1086797/1‑140 (MQ=255)
                                                                                aaaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGa                                                             <  1:591533/87‑1 (MQ=255)
                                                                                aaaGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGa                                                             >  2:591533/1‑87 (MQ=255)
                                                                                    ttGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaa                                          <  4:874535/102‑1 (MQ=255)
                                                                                    ttGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaa                                          >  3:874535/1‑102 (MQ=255)
                                                                                         cAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaa                                                                      >  1:395712/1‑69 (MQ=255)
                                                                                         cAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTtaa                                                                      <  2:395712/69‑1 (MQ=255)
                                                                                           aCGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaaa                                         >  2:532594/1‑96 (MQ=255)
                                                                                           aCGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaaa                                         <  1:532594/96‑1 (MQ=255)
                                                                                           aCGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaaa                                         >  4:925222/1‑96 (MQ=255)
                                                                                           aCGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaaa                                         <  3:925222/96‑1 (MQ=255)
                                                                                            cGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaa                                          >  2:493786/1‑94 (MQ=255)
                                                                                            cGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGaa                                          <  1:493786/94‑1 (MQ=255)
                                                                                                          tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAacaaca                                                       >  2:751747/1‑67 (MQ=255)
                                                                                                          tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAacaaca                                                       <  1:751747/67‑1 (MQ=255)
                                                                                                          tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcgg                                  <  2:551383/88‑1 (MQ=255)
                                                                                                          tAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAAcgg                                  >  1:551383/1‑88 (MQ=255)
                                                                                                           aaCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCa                      <  2:479860/99‑1 (MQ=255)
                                                                                                           aaCGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCa                      >  1:479860/1‑99 (MQ=255)
                                                                                                                      tgatgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                    >  3:953799/1‑90 (MQ=255)
                                                                                                                      tgatgCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAtt                    <  4:953799/90‑1 (MQ=255)
                                                                                                                            atatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattta  <  2:544020/102‑1 (MQ=255)
                                                                                                                            atatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattta  >  1:544020/1‑102 (MQ=255)
                                                                                                                            atatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattt   >  2:943854/1‑101 (MQ=255)
                                                                                                                            atatCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATAtattt   <  1:943854/101‑1 (MQ=255)
                                                                                                                                 ctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAt                     <  3:426373/78‑1 (MQ=255)
                                                                                                                                 ctccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCAt                     >  4:426373/1‑78 (MQ=255)
                                                                                                                                  tccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGt                  >  2:694379/1‑80 (MQ=255)
                                                                                                                                  tccGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGt                  <  1:694379/80‑1 (MQ=255)
                                                                                                                                                  |                                                                               
GGTTCTGTGGTTTCGGTGGTAGAAAAGCGCAAAACATCGCTGCTTTCCGTCAACCGCCAGTATGGATTGTGCGATCCGAGAAAGTTGTACAACGTTGTGGACTCCCTAACGGTTTTCATGATGCATATCCTCCGGTTAACAGCGGAGTCCGCGGTTAAATAAAAGGAACAACATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTA  >  NC_000913/1346624‑1346849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: