Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,706,745 T→A 5.8% V133D (GTT→GAT)  rsxB → SoxR iron‑sulfur cluster reduction factor component; putative iron‑sulfur protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,706,7450TA 5.8% 255.1 / 5.4 69V133D (GTT→GAT) rsxBSoxR iron‑sulfur cluster reduction factor component; putative iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base T (33/32);  new base A (2/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

AAATTGCCGAGTTGCTTAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACAC  >  NC_000913/1706598‑1706886
                                                                                                                                                   |                                                                                                                                              
aaaTTGCCGAGTTGCTTAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGt                                                                                                                                                <  3:309557/148‑1 (MQ=255)
                tAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGcc                                                                                                                               >  4:806568/1‑148 (MQ=255)
                               gccgcTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                <  3:806568/148‑1 (MQ=255)
                                                   aaGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATa                                                                                                                        >  1:307241/1‑120 (MQ=255)
                                                   aaGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATa                                                                                                                        <  2:307241/120‑1 (MQ=255)
                                                    agagATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAga                                                                                                             <  1:781076/130‑2 (MQ=255)
                                                    agagATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGt                                                                                                             >  2:781076/1‑130 (MQ=255)
                                                         tAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAttt                                                                                      >  2:210608/1‑148 (MQ=255)
                                                         tAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAttt                                                                                      >  3:226049/1‑148 (MQ=255)
                                                           aCGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                <  2:8497/120‑1 (MQ=255)
                                                           aCGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                >  1:8497/1‑120 (MQ=255)
                                                            cGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   <  4:226049/148‑1 (MQ=255)
                                                                tGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAg                                                                                                              >  2:449358/1‑117 (MQ=255)
                                                                tGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAg                                                                                                              <  1:449358/117‑1 (MQ=255)
                                                                    cgGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCa                                                                                          <  3:274243/133‑1 (MQ=255)
                                                                    cgGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCa                                                                                          >  4:274243/1‑133 (MQ=255)
                                                                             gCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGa                                                                  <  1:210608/148‑1 (MQ=255)
                                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGAtctc                                                                                                       <  3:1131885/110‑1 (MQ=255)
                                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGAtctc                                                                                                       >  4:1131885/1‑110 (MQ=255)
                                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              <  2:585512/119‑1 (MQ=255)
                                                                              cGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              >  1:585512/1‑119 (MQ=255)
                                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTaa                                                                                                                  <  2:954881/89‑1 (MQ=255)
                                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTaa                                                                                                                  >  1:954881/1‑89 (MQ=255)
                                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              <  4:1065318/109‑1 (MQ=255)
                                                                                        tgaAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGc                                                                                              >  3:1065318/1‑109 (MQ=255)
                                                                                          aaaaTAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATg                                                                                                                            >  1:559304/1‑77 (MQ=255)
                                                                                          aaaaTAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATg                                                                                                                            <  2:559304/77‑1 (MQ=255)
                                                                                                       tGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCa                                               >  4:600704/1‑141 (MQ=255)
                                                                                                       tGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCa                                               <  3:600704/141‑1 (MQ=255)
                                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGc                                                                                                                           <  1:833329/62‑1 (MQ=255)
                                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGc                                                                                                                           >  2:833329/1‑62 (MQ=255)
                                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACggg                                                                                               >  2:247089/1‑90 (MQ=255)
                                                                                                          cTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACggg                                                                                               <  1:247089/90‑1 (MQ=255)
                                                                                                            gCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGa                                   >  2:742325/1‑148 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                <  1:199881/67‑1 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                >  2:199881/1‑67 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAt                                                        <  4:680909/123‑1 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAt                                                        >  3:680909/1‑123 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  4:755101/148‑1 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               >  3:755101/1‑148 (MQ=255)
                                                                                                                tAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  1:742325/148‑1 (MQ=255)
                                                                                                                 aaaTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   >  1:36790/1‑95 (MQ=255)
                                                                                                                 aaaTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTAtg                                                                                   <  2:36790/95‑1 (MQ=255)
                                                                                                                   aTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      <  2:708193/122‑1 (MQ=255)
                                                                                                                   aTGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      >  1:708193/1‑122 (MQ=255)
                                                                                                                    tGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                >  2:747198/1‑63 (MQ=255)
                                                                                                                    tGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATg                                                                                                                <  1:747198/63‑1 (MQ=255)
                                                                                                                      tATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACg                                                                <  3:1076783/109‑1 (MQ=255)
                                                                                                                      tATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACg                                                                >  4:1076783/1‑109 (MQ=255)
                                                                                                                          cAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCt                     >  1:737005/1‑148 (MQ=255)
                                                                                                                            ggCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               >  1:352314/1‑136 (MQ=255)
                                                                                                                            ggCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAaca                               <  2:352314/136‑1 (MQ=255)
                                                                                                                              cGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  1:789413/1‑148 (MQ=255)
                                                                                                                                    cGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           <  2:737005/148‑1 (MQ=255)
                                                                                                                                      gTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      >  3:25165/1‑103 (MQ=255)
                                                                                                                                      gTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCAtct                                                      <  4:25165/103‑1 (MQ=255)
                                                                                                                                       tAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGAt          <  3:264745/146‑1 (MQ=255)
                                                                                                                                       tAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGAt          >  4:264745/1‑146 (MQ=255)
                                                                                                                                        tagcGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  2:150671/4‑139 (MQ=255)
                                                                                                                                        tagcGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 <  1:150671/136‑1 (MQ=255)
                                                                                                                                        aGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTg       >  3:875868/1‑148 (MQ=255)
                                                                                                                                           cGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           >  4:270824/1‑142 (MQ=255)
                                                                                                                                           cGCCAACGATGGCGTCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGa           <  3:270824/142‑1 (MQ=255)
                                                                                                                                             ccATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAacac  <  2:789413/148‑1 (MQ=255)
                                                                                                                                              cATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAt                                                                                        <  3:734003/61‑1 (MQ=255)
                                                                                                                                              cATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAAt                                                                                        >  4:734003/1‑61 (MQ=255)
                                                                                                                                               aTCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 >  1:420870/1‑131 (MQ=255)
                                                                                                                                               aTCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGaa                 <  2:420870/131‑1 (MQ=255)
                                                                                                                                               aTCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              <  2:266905/134‑1 (MQ=255)
                                                                                                                                               aTCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATg              >  1:266905/1‑134 (MQ=255)
                                                                                                                                                   ttGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATggg            >  4:159402/1‑132 (MQ=255)
                                                                                                                                                   ttGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATggg            <  3:159402/132‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AAATTGCCGAGTTGCTTAATGTCGAGCCGCAGCCGCTGGATGGCGAAGCGCAAGAGATAACGCCTGCGCGGATGGTGGCGGTTATTGATGAAAATAACTGTATTGGCTGCACTAAATGTATTCAGGCGTGTCCGGTAGACGCCATCGTTGGCGCTACCCGTGCCATGCATACGGTAATGAGTGATCTCTGTACGGGCTGCAATTTATGTGTTGATCCGTGCCCGACGCACTGCATCTCGTTGCAACCGGTCGCAGAAACACCTGACTCCTGGAAATGGGATCTGAACAC  >  NC_000913/1706598‑1706886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: