Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,736,097 | T→A | 11.1% | intergenic (+138/+24) | sodB → / ← ydhP | superoxide dismutase, Fe/putative MFS transporter, inner membrane protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,736,097 | 0 | T | A | 11.1% | 175.1 / 12.4 | 55 | intergenic (+138/+24) | sodB/ydhP | superoxide dismutase, Fe/putative MFS transporter, inner membrane protein |
Reads supporting (aligned to +/- strand): ref base T (24/24); new base A (3/3); total (28/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 |
CAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGG > NC_000913/1735970‑1736242 | cAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATc < 4:1075482/132‑1 (MQ=255) cAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGGTATc > 3:1075482/1‑132 (MQ=255) aaGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt < 1:826724/117‑1 (MQ=255) aaGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt > 2:826724/1‑117 (MQ=255) cTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATTAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGtt < 2:299879/106‑1 (MQ=255) cTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGTAACTGTAACGCAAAAGGtt > 1:299879/1‑106 (MQ=255) aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt < 3:5601/122‑1 (MQ=255) aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt > 4:5601/1‑122 (MQ=255) aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa < 4:1013595/136‑1 (MQ=255) aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa > 3:1013595/1‑136 (MQ=255) cGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc > 3:932996/1‑110 (MQ=255) cGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAAATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc < 4:932996/110‑1 (MQ=255) cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa < 3:41211/122‑1 (MQ=255) cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa > 4:41211/1‑122 (MQ=255) cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa > 2:921791/1‑143 (MQ=255) cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa < 1:921791/143‑1 (MQ=255) tATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTc > 1:190118/1‑148 (MQ=255) cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt < 4:1021998/98‑1 (MQ=255) cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt > 3:1021998/1‑98 (MQ=255) cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt > 3:224383/1‑127 (MQ=255) cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt < 4:224383/127‑1 (MQ=255) gCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttg > 4:224363/1‑148 (MQ=255) cgcgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTc < 2:190118/148‑1 (MQ=255) gATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGc < 3:224363/148‑1 (MQ=255) atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg > 4:711912/1‑92 (MQ=255) atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg < 3:711912/92‑1 (MQ=255) tcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCt < 4:596067/84‑1 (MQ=255) tcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCt > 3:596067/1‑84 (MQ=255) aTGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGttt < 2:52016/124‑1 (MQ=255) aTGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGttt > 1:52016/1‑124 (MQ=255) gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg < 3:294233/75‑1 (MQ=255) gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg > 4:294233/1‑75 (MQ=38) aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATaa < 2:464916/137‑1 (MQ=255) aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATaa > 1:464916/1‑137 (MQ=255) aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaa < 4:986162/104‑1 (MQ=255) aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaa > 3:986162/1‑104 (MQ=255) gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc < 3:292218/87‑1 (MQ=255) gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc > 4:292218/1‑87 (MQ=255) cgccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTc > 3:470820/1‑148 (MQ=255) cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt < 4:1093425/99‑1 (MQ=255) cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt > 3:1093425/1‑99 (MQ=255) ggCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaa > 3:740184/1‑127 (MQ=255) ggCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaa < 4:740184/127‑1 (MQ=255) gCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt < 1:1094846/97‑1 (MQ=255) gCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt > 2:1094846/1‑97 (MQ=255) cGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAc < 4:470820/148‑1 (MQ=255) ttGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGcac > 1:318676/1‑148 (MQ=255) gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg < 2:809313/83‑1 (MQ=255) gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg > 1:809313/1‑83 (MQ=255) cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg < 2:42578/88‑1 (MQ=255) cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg > 1:42578/1‑88 (MQ=255) cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCt < 4:414876/116‑1 (MQ=255) cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCt > 3:414876/1‑116 (MQ=255) cAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaata > 4:234163/1‑93 (MQ=255) cAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaata < 3:234163/93‑1 (MQ=255) gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCgg > 4:3614/1‑147 (MQ=255) gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCgg < 3:3614/147‑1 (MQ=255) | CAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGG > NC_000913/1735970‑1736242 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |