Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,736,100 T→A 11.3% intergenic (+141/+21) sodB → / ← ydhP superoxide dismutase, Fe/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,1000TA11.3% 170.7 / 12.2 53intergenic (+141/+21)sodB/ydhPsuperoxide dismutase, Fe/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base A (3/3);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGG  >  NC_000913/1735970‑1736242
                                                                                                                                  |                                                                                                                                              
cAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATc                                                                                                                                               <  4:1075482/132‑1 (MQ=255)
cAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGGTATc                                                                                                                                               >  3:1075482/1‑132 (MQ=255)
                  aaGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt                                                                                                                                            <  1:826724/117‑1 (MQ=255)
                  aaGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCtt                                                                                                                                            >  2:826724/1‑117 (MQ=255)
                         aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                >  4:5601/1‑122 (MQ=255)
                         aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGt                                                                                                                                <  3:5601/122‑1 (MQ=255)
                         aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa                                                                                                                  <  4:1013595/136‑1 (MQ=255)
                         aCGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa                                                                                                                  >  3:1013595/1‑136 (MQ=255)
                          cGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                           >  3:932996/1‑110 (MQ=255)
                          cGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAAATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTc                                                                                                                                           <  4:932996/110‑1 (MQ=255)
                            cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa                                                                                                                             >  4:41211/1‑122 (MQ=255)
                            cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTa                                                                                                                             <  3:41211/122‑1 (MQ=255)
                            cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                        <  1:921791/143‑1 (MQ=255)
                            cTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaaa                                                                                                        >  2:921791/1‑143 (MQ=255)
                              tATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTc                                                                                                 >  1:190118/1‑148 (MQ=255)
                                 cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                                                                                                >  3:1021998/1‑98 (MQ=255)
                                 cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTAt                                                                                                                                                <  4:1021998/98‑1 (MQ=255)
                                 cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt                                                                                                                   >  3:224383/1‑127 (MQ=255)
                                 cAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGtt                                                                                                                   <  4:224383/127‑1 (MQ=255)
                                    gCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttg                                                                                           >  4:224363/1‑148 (MQ=255)
                                         cgcgGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTc                                                                                      <  2:190118/148‑1 (MQ=255)
                                             gATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGc                                                                                  <  3:224363/148‑1 (MQ=255)
                                              atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg                                                                                                                                         <  3:711912/92‑1 (MQ=255)
                                              atcatcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg                                                                                                                                         >  4:711912/1‑92 (MQ=255)
                                                  tcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCt                                                                                                                                             >  3:596067/1‑84 (MQ=255)
                                                  tcGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCt                                                                                                                                             <  4:596067/84‑1 (MQ=255)
                                                     aTGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGttt                                                                                                  <  2:52016/124‑1 (MQ=255)
                                                     aTGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGttt                                                                                                  >  1:52016/1‑124 (MQ=255)
                                                               gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg                                                                                                                                         <  3:294233/75‑1 (MQ=255)
                                                               gATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGATAACCTTCTg                                                                                                                                         >  4:294233/1‑75 (MQ=38)
                                                                aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATaa                                                                          >  1:464916/1‑137 (MQ=255)
                                                                aTAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATaa                                                                          <  2:464916/137‑1 (MQ=255)
                                                                  aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaa                                                                                                         <  4:986162/104‑1 (MQ=255)
                                                                  aaGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCaa                                                                                                         >  3:986162/1‑104 (MQ=255)
                                                                     gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc                                                                                                                       <  3:292218/87‑1 (MQ=255)
                                                                     gCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGc                                                                                                                       >  4:292218/1‑87 (MQ=255)
                                                                          cgccgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTc                                                     >  3:470820/1‑148 (MQ=255)
                                                                             cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt                                                                                                   <  4:1093425/99‑1 (MQ=255)
                                                                             cgcATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGtt                                                                                                   >  3:1093425/1‑99 (MQ=255)
                                                                                    ggCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaa                                                                >  3:740184/1‑127 (MQ=255)
                                                                                    ggCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaa                                                                <  4:740184/127‑1 (MQ=255)
                                                                                     gCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt                                                                                             >  2:1094846/1‑97 (MQ=255)
                                                                                     gCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgt                                                                                             <  1:1094846/97‑1 (MQ=255)
                                                                                                cGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCAc                               <  4:470820/148‑1 (MQ=255)
                                                                                                      ttGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGcac                         >  1:318676/1‑148 (MQ=255)
                                                                                                            gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg                                                                                    <  2:809313/83‑1 (MQ=255)
                                                                                                            gCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg                                                                                    >  1:809313/1‑83 (MQ=255)
                                                                                                                        cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg                                                                   >  1:42578/1‑88 (MQ=255)
                                                                                                                        cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg                                                                   <  2:42578/88‑1 (MQ=255)
                                                                                                                        cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCt                                       <  4:414876/116‑1 (MQ=255)
                                                                                                                        cAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCt                                       >  3:414876/1‑116 (MQ=255)
                                                                                                                        cAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaata                                                              <  3:234163/93‑1 (MQ=255)
                                                                                                                        cAGAAGGATAACCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaata                                                              >  4:234163/1‑93 (MQ=255)
                                                                                                                              gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCgg  >  4:3614/1‑147 (MQ=255)
                                                                                                                              gTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCgg  <  3:3614/147‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                              
CAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCATCGATGTAGGTCGGATAAGGCACTCGCCGCATCCGGCAAGATAAATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGACAATCGCTCCCATCACCGGCACAAAGCTGTATCCCAGCCCAGCGG  >  NC_000913/1735970‑1736242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: