| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,772,475 | C→T | 6.1% | intergenic (+190/‑37) | ydiM → / → ydiN | putative MFS transporter, membrane protein/putative MFS transporter, membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,772,475 | 0 | C | T | 6.1% | 208.7 / 4.0 | 65 | intergenic (+190/‑37) | ydiM/ydiN | putative MFS transporter, membrane protein/putative MFS transporter, membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (32/29); new base T (2/2); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
AAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTGCCCAAAATA > NC_000913/1772347‑1772617 | aaGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACg < 1:1093636/136‑1 (MQ=255)aaGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACg > 2:1093636/1‑136 (MQ=255) aTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCtgt > 4:666744/1‑148 (MQ=255) aCATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATa < 3:666744/148‑1 (MQ=255) cATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCATCAAGGAGc > 1:287747/1‑127 (MQ=255) cATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGc < 2:287747/127‑1 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCttt > 2:444587/1‑113 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCttt < 1:444587/113‑1 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTc < 2:477433/114‑1 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTc > 1:477433/1‑114 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCaaa > 1:1020622/1‑141 (MQ=255) gCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCaaa < 2:1020622/141‑1 (MQ=255) cAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCaa > 2:326829/1‑118 (MQ=255) cAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCaa < 1:326829/118‑1 (MQ=255) aaaCATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCtg > 2:108803/1‑113 (MQ=255) aaaCATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCtg < 1:108803/113‑1 (MQ=255) tAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTGTGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg < 1:450096/116‑1 (MQ=255) tAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTGTGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg > 2:450096/1‑116 (MQ=255) aaaCTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCtt > 2:79385/1‑93 (MQ=255) aaaCTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCtt < 1:79385/93‑1 (MQ=255) aaCTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTc < 2:42848/94‑1 (MQ=255) aaCTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTc > 1:42848/1‑94 (MQ=255) ttATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGc > 1:321111/1‑148 (MQ=255) tGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCttt < 2:321111/148‑1 (MQ=255) gTACCCAGGGTAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTAt > 1:185983/1‑76 (MQ=255) gTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTAt < 2:185983/76‑1 (MQ=255) cAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg < 3:442550/96‑1 (MQ=255) cAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg > 4:442550/1‑96 (MQ=255) cAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCtt > 1:454053/1‑141 (MQ=255) cAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCtt < 2:454053/141‑1 (MQ=255) ggAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATaa > 2:201175/1‑104 (MQ=255) ggAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATaa < 1:201175/104‑1 (MQ=255) ggAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTAt > 4:436022/1‑125 (MQ=255) ggAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTAt < 3:436022/125‑1 (MQ=255) atcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGaaaa < 3:634522/86‑1 (MQ=255) atcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGaaaa > 4:634522/1‑86 (MQ=255) atcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCa > 4:272837/1‑110 (MQ=255) atcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCa < 3:272837/110‑1 (MQ=255) tcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGtct < 1:1049205/90‑1 (MQ=255) tcatcTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGtct > 2:1049205/1‑90 (MQ=255) tCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg > 4:557275/1‑81 (MQ=255) tCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATg < 3:557275/81‑1 (MQ=255) taGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATc < 3:987937/108‑1 (MQ=255) taGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATc > 4:987937/1‑108 (MQ=255) tAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGTCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCa > 1:406595/1‑148 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTATGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCttt < 2:664213/85‑1 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTATGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCttt > 1:664213/1‑85 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTATGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCttt > 3:679858/1‑85 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTATGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCttt < 4:679858/85‑1 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCAtt > 3:169231/1‑146 (MQ=255) tATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCAtt < 4:169231/146‑1 (MQ=255) tAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCa < 3:1018458/135‑1 (MQ=255) tAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCa > 4:1018458/1‑135 (MQ=255) aaaCCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGtta > 1:163589/1‑148 (MQ=255) aCCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCtt > 2:454139/1‑77 (MQ=255) aCCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCtt < 1:454139/77‑1 (MQ=255) aCCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCa > 3:222505/1‑137 (MQ=255) aCCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCa < 4:222505/137‑1 (MQ=255) agaACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTg > 2:674174/1‑147 (MQ=255) agaACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTg < 1:674174/147‑1 (MQ=255) aaCAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGc > 4:320323/1‑72 (MQ=255) aaCAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGc < 3:320323/72‑1 (MQ=255) aCAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTGCCCa > 3:308937/1‑148 (MQ=255) aaTACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGtt < 3:1086136/132‑1 (MQ=255) aaTACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGtt > 4:1086136/1‑132 (MQ=255) acgtacgGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTGCCCAAAata < 2:163589/148‑1 (MQ=255) acgtacgGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTGCCCAAAata < 2:406595/148‑1 (MQ=255) | AAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGAAAATGTCTCAAAATAAGGCTTTCAGCACGCCATTTATCCTGGCTGTTCTTTGTATTTACTTCAGCTACTTCCTGCACGGCATTAGTGTTATTACGCTTGCCCAAAATA > NC_000913/1772347‑1772617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |