| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,793,668 | C→A | 11.4% | E148* (GAA→TAA) ‡ | btuE ← | glutathione peroxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,793,668 | 0 | C | A | 11.4% | 113.4 / 6.7 | 35 | E148* (GAA→TAA) ‡ | btuE | glutathione peroxidase |
| Reads supporting (aligned to +/- strand): ref base C (16/15); new base A (2/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAA > NC_000913/1793558‑1793814 | ttattTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTcc < 3:160656/119‑1 (MQ=255)ttattTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTcc > 4:160656/1‑119 (MQ=255) tattTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTGAAAAATTc < 1:21363/117‑1 (MQ=255) tattTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTGAAAAATTc > 2:21363/1‑117 (MQ=255) tCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATatcat < 3:120313/99‑1 (MQ=255) tCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATatcat > 4:120313/1‑99 (MQ=255) cacaATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCg > 4:418147/1‑148 (MQ=255) aTGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaa > 1:1037665/1‑77 (MQ=255) aTGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaa < 2:1037665/77‑1 (MQ=255) gggATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGc < 1:822970/103‑1 (MQ=255) gggATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGc > 2:822970/1‑103 (MQ=255) gggATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTGAAAAATTcctg < 2:207468/82‑3 (MQ=255) gggATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTGAAAAATTcctg > 1:207468/1‑80 (MQ=255) gATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaa < 3:399172/73‑1 (MQ=255) gATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaa > 4:399172/1‑73 (MQ=255) ccGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCAt > 4:266273/1‑114 (MQ=255) ccGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCAt > 4:313291/1‑114 (MQ=255) ccGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCAt < 3:266273/114‑1 (MQ=255) ccGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCAt < 3:313291/114‑1 (MQ=255) cTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGctc < 2:775968/112‑1 (MQ=255) cTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGctc > 1:775968/1‑112 (MQ=255) tttCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTg > 3:570945/1‑78 (MQ=255) tttCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTg < 4:570945/78‑1 (MQ=255) tCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCg < 3:737287/114‑1 (MQ=255) tCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCg > 4:737287/1‑114 (MQ=255) cccTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGgcg < 2:562081/105‑1 (MQ=255) cccTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGgcg > 1:562081/1‑105 (MQ=255) cTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAAt < 3:423066/87‑1 (MQ=255) cTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAAt > 4:423066/1‑87 (MQ=255) tGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATg > 3:1082474/1‑148 (MQ=255) aaTTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGcc < 3:314024/50‑1 (MQ=255) aaTTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGcc > 4:314024/1‑50 (MQ=255) tttttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAAttttt > 1:582991/1‑122 (MQ=255) tttttCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAAttttt < 2:582991/122‑1 (MQ=255) tCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTaa < 4:1082474/148‑1 (MQ=255) | TTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAATTCCATAAAATATCATCCGGGTACAGCGGTGCACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAA > NC_000913/1793558‑1793814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |