Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,827,816 C→A 8.6% L148L (CTG→CTT)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,827,8160CA 8.6% 239.8 / 11.8 70L148L (CTG→CTT) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base A (4/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

AAAAGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAA  >  NC_000913/1827679‑1827957
                                                                                                                                         |                                                                                                                                             
aaaaGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAg                                                                                                                                     >  3:474021/1‑148 (MQ=255)
   aGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGa                                                                                                                                  >  4:725085/1‑148 (MQ=255)
       gCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACggg                                                                                                                              <  3:725085/148‑1 (MQ=255)
            cccGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACg                                                                                                                                >  1:926523/1‑141 (MQ=255)
            cccGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACg                                                                                                                                <  2:926523/141‑1 (MQ=255)
             ccGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGa                                                                                                                                          <  1:1000690/130‑1 (MQ=255)
             ccGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGa                                                                                                                                          >  2:1000690/1‑130 (MQ=255)
              cGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGgcg                                                                                                                            >  2:1084416/1‑143 (MQ=255)
              cGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGgcg                                                                                                                            <  1:1084416/143‑1 (MQ=255)
                gTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAg                                                                                                                     <  1:500521/148‑1 (MQ=255)
                 ttCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGc                                                                                                                    >  2:260574/1‑148 (MQ=255)
                               cgccgAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAAttt                                                                                                      <  4:474021/148‑1 (MQ=255)
                                    agaCGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCa                                                                                                                                      <  1:29033/111‑1 (MQ=255)
                                    agaCGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCa                                                                                                                                      >  2:29033/1‑111 (MQ=255)
                                         aTTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAgg                                                                                            >  3:801477/1‑148 (MQ=255)
                                                tttGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGa                                                                                                                  >  2:1083336/1‑119 (MQ=255)
                                                tttGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGa                                                                                                                  <  1:1083336/119‑1 (MQ=255)
                                                    ccgccCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAAt                                                                                                        <  4:724896/125‑1 (MQ=255)
                                                    ccgccCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAAt                                                                                                        >  3:724896/1‑125 (MQ=255)
                                                      gccCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga                                                                               <  4:801477/148‑1 (MQ=255)
                                                        ccccTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTgaga                                                                             <  1:260574/148‑1 (MQ=255)
                                                            tcatcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCtt                                                                                                                         >  2:1041364/1‑100 (MQ=255)
                                                            tcatcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCtt                                                                                                                         <  1:1041364/100‑1 (MQ=255)
                                                              atcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTcc                                                                                                                       <  3:1036418/100‑1 (MQ=255)
                                                              atcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTcc                                                                                                                       >  4:1036418/1‑100 (MQ=255)
                                                              atcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACt                                                                       >  2:613988/1‑148 (MQ=255)
                                                               tcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTc                                                                                               <  1:383131/123‑1 (MQ=255)
                                                               tcaCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTc                                                                                               >  2:383131/1‑123 (MQ=255)
                                                                       cAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTAt                                                                                                    >  4:702031/1‑110 (MQ=255)
                                                                       cAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTAt                                                                                                    <  3:702031/110‑1 (MQ=255)
                                                                       cAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATcc                                                              <  1:613988/148‑1 (MQ=255)
                                                                              aCCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTAt                                                       >  4:290607/1‑148 (MQ=255)
                                                                                     gCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGa                                                <  3:290607/148‑1 (MQ=255)
                                                                                        aCGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACa                                                                                                                                              <  4:715513/51‑1 (MQ=255)
                                                                                        aCGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACa                                                                                                                                              >  3:715513/1‑51 (MQ=255)
                                                                                                      cTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga                                                                               >  3:710612/1‑100 (MQ=255)
                                                                                                      cTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTga                                                                               <  4:710612/100‑1 (MQ=255)
                                                                                                      cTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGAt                                       >  4:1112427/1‑140 (MQ=255)
                                                                                                      cTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGAt                                       <  3:1112427/140‑1 (MQ=255)
                                                                                                              ctctTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGt                                    >  4:436401/1‑135 (MQ=255)
                                                                                                              ctctTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGt                                    <  3:436401/135‑1 (MQ=255)
                                                                                                                ctTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGa                                                                                                                  <  4:330383/55‑1 (MQ=255)
                                                                                                                ctTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGa                                                                                                                  >  3:330383/1‑55 (MQ=255)
                                                                                                                  tcgtcgTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACg                                                                                                               >  3:235077/1‑56 (MQ=255)
                                                                                                                  tcgtcgTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACg                                                                                                               <  4:235077/56‑1 (MQ=255)
                                                                                                                  tcgtcgTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTAccc                   >  4:149344/1‑148 (MQ=255)
                                                                                                                     tcgtTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCtt                                                                                                                         <  3:538550/43‑1 (MQ=255)
                                                                                                                     tcgtTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCtt                                                                                                                         >  4:538550/1‑43 (MQ=255)
                                                                                                                     tcgtTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATg                                                                           <  1:512682/89‑1 (MQ=255)
                                                                                                                     tcgtTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATg                                                                           >  2:512682/1‑89 (MQ=255)
                                                                                                                      cgtTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAt               >  1:229432/1‑148 (MQ=255)
                                                                                                                         tAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACtt                                                                      <  3:238343/90‑1 (MQ=255)
                                                                                                                         tAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACtt                                                                      >  4:238343/1‑90 (MQ=255)
                                                                                                                         tAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTgg            >  3:289284/1‑148 (MQ=255)
                                                                                                                            gaaTCGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCg                                                     >  4:1040591/2‑104 (MQ=255)
                                                                                                                            gaaTCGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCg                                                     <  3:1040591/103‑1 (MQ=255)
                                                                                                                            gaaTCGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGa         >  3:473340/2‑148 (MQ=255)
                                                                                                                            aaaTCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGa         <  4:289284/148‑1 (MQ=255)
                                                                                                                              aTCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAg       <  3:149344/148‑1 (MQ=255)
                                                                                                                                cGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCg     >  4:29422/1‑148 (MQ=255)
                                                                                                                                cGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAtt              >  3:405539/1‑139 (MQ=255)
                                                                                                                                cGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACAtt              <  4:405539/139‑1 (MQ=255)
                                                                                                                                cGCTGTTAAAAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCg     >  4:422702/1‑148 (MQ=255)
                                                                                                                                   ttttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGaa  >  4:735992/1‑148 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGc                                                                          <  2:938116/75‑1 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGc                                                                          >  1:938116/1‑75 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCAt                                                                <  2:205769/85‑1 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCAt                                                                >  1:205769/1‑85 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCgg                                                    <  3:915149/97‑1 (MQ=255)
                                                                                                                                    tttAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCgg                                                    >  4:915149/1‑97 (MQ=255)
                                                                                                                                         cAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGAt                                       <  4:206328/105‑1 (MQ=255)
                                                                                                                                         cAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGAt                                       >  3:206328/1‑105 (MQ=255)
                                                                                                                                         |                                                                                                                                             
AAAAGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAA  >  NC_000913/1827679‑1827957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑