Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 167,293 G→C 37.9% intergenic (+29/‑191) mrcB → / → fhuA fused glycosyl transferase and transpeptidase/ferrichrome outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913167,2930GC37.9% 25.1 / 33.3 29intergenic (+29/‑191)mrcB/fhuAfused glycosyl transferase and transpeptidase/ferrichrome outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (8/10);  new base C (4/7);  total (12/17)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

TCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAACCCGCCAGCGTTT  >  NC_000913/167147‑167411
                                                                                                                                                  |                                                                                                                      
tCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       <  1:272366/148‑1 (MQ=255)
tCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       <  1:421987/148‑1 (MQ=255)
             tcttctCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       >  2:16793/1‑135 (MQ=255)
             tcttctCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       <  1:16793/135‑1 (MQ=255)
                   cAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATcc                                                                                                               <  1:1017295/137‑1 (MQ=255)
                   cAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATcc                                                                                                               >  2:1017295/1‑137 (MQ=255)
                                       aCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCAt                                                                                                                 <  1:1029634/115‑1 (MQ=255)
                                       aCAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCAt                                                                                                                 >  2:1029634/1‑115 (MQ=255)
                                                    cAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       <  2:239187/96‑1 (MQ=255)
                                                    cAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGt                                                                                                                       >  1:239187/1‑96 (MQ=255)
                                                                aaaGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGtt                                                                                                                      <  2:148136/85‑1 (MQ=255)
                                                                aaaGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGtt                                                                                                                      >  1:148136/1‑85 (MQ=255)
                                                                   gACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                <  2:650999/88‑1 (MQ=255)
                                                                       gCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                >  4:243296/1‑84 (MQ=39)
                                                                       gCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                <  3:243574/84‑1 (MQ=39)
                                                                       gCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                <  3:243296/84‑1 (MQ=39)
                                                                          aCGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAg                                                                                                                        <  2:1082991/73‑1 (MQ=255)
                                                                          aCGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAg                                                                                                                        >  1:1082991/1‑73 (MQ=255)
                                                                           cGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                <  1:87100/80‑1 (MQ=39)
                                                                           cGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCAACTCGCCATc                                                                                                                >  2:87100/1‑80 (MQ=39)
                                                                                                        ttGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGata                                                                                     >  4:81460/1‑78 (MQ=255)
                                                                                                        ttGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACACCTCATCTAAGata                                                                                     <  3:81460/78‑1 (MQ=255)
                                                                                                                                         ggATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTc                                              <  2:616017/84‑1 (MQ=255)
                                                                                                                                         ggATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTc                                              >  1:616017/1‑84 (MQ=255)
                                                                                                                                         ggATGGCAACTCGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGgttgt                                                           <  2:1083710/71‑1 (MQ=38)
                                                                                                                                          gATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTc                                              <  2:355497/83‑1 (MQ=255)
                                                                                                                                          gATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTc                                              >  1:355497/1‑83 (MQ=255)
                                                                                                                                          gATGGCAACTCGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAACCCGCCAGCGttt  >  3:716393/1‑127 (MQ=255)
                                                                                                                                          gATGGCAACTCGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAACCCGCCAGCGttt  <  4:716393/127‑1 (MQ=255)
                                                                                                                                                 aGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAAcc             >  3:1062922/1‑109 (MQ=255)
                                                                                                                                                 aGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAAcc             <  4:1062922/109‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                      
TCCGTTTGATCAGTCTTCTCAGCCGCAGCAACAGCCGCAACAGCAACCTGCTCAGCAAGAGCAGAAAGACAGCGACGGTGTAGCCGGTTGGATCAAGGATATGTTTGGTAGTAATTAACATCTAAGCGTGAAATACCGGATGGCGAGTTGCCATCCGGTAAAATAACATCCCATCTAAGATATTAACCCTTTCTTTTCATCTGGTTGTTTATTAACCCTTCAGGAACGCTCAGATTGCGTACCGCTTGCGAACCCGCCAGCGTTT  >  NC_000913/167147‑167411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: