Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,948,180 T→A 6.7% *151C (TGA→TGT nudB ← dihydroneopterin triphosphate pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,948,1800TA 6.7% 212.1 / 5.6 60*151C (TGA→TGTnudBdihydroneopterin triphosphate pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base A (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01

CCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAA  >  NC_000913/1948037‑1948319
                                                                                                                                               |                                                                                                                                           
ccAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                                                                                                                                         <  2:1064777/148‑1 (MQ=255)
                    aGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     <  1:878950/148‑1 (MQ=255)
                          cGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAAt                                                                                                                <  2:991924/147‑1 (MQ=255)
                          cGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAAt                                                                                                                >  1:991924/1‑147 (MQ=255)
                            aaTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAACCTGt                                                                                                                     <  4:436388/140‑1 (MQ=255)
                            aaTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     >  3:436388/1‑140 (MQ=255)
                                  tttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                                                                                                                                         >  4:933435/1‑114 (MQ=255)
                                  tttAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAgg                                                                                                                                         <  3:933435/114‑1 (MQ=255)
                                       tGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGc                                                                                                  >  3:521513/1‑148 (MQ=255)
                                        gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc                                                                                                                                            <  4:691761/105‑1 (MQ=255)
                                        gAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc                                                                                                                                            >  3:691761/1‑105 (MQ=255)
                                          aGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCgg                                                                                                      <  3:1040670/141‑1 (MQ=255)
                                          aGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCgg                                                                                                      >  4:1040670/1‑141 (MQ=255)
                                           gATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCa                                                                                              <  4:521513/148‑1 (MQ=255)
                                                 aCCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACtt                                                                                        >  4:1072489/1‑148 (MQ=255)
                                                   cgcgCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     <  2:848858/117‑1 (MQ=255)
                                                   cgcgCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGCTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     >  1:848858/1‑117 (MQ=255)
                                                           gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc                                                                                                                                            <  1:33319/86‑1 (MQ=255)
                                                           gCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc                                                                                                                                            >  2:33319/1‑86 (MQ=255)
                                                               ccTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa                                                                                                                                >  1:205251/1‑94 (MQ=255)
                                                               ccTGCGCAGCTTTACGATGTCTGGTGGTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAACGTTaa                                                                                                                                <  2:205251/94‑1 (MQ=255)
                                                                     cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGc                                                                                                  <  3:883772/118‑1 (MQ=255)
                                                                     cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGc                                                                                                  >  4:883772/1‑118 (MQ=255)
                                                                     cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGcagcag                                                                    <  3:1072489/148‑1 (MQ=255)
                                                                     cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAg                                                                                                                                      <  3:882913/82‑1 (MQ=255)
                                                                     cAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAg                                                                                                                                      >  4:882913/1‑82 (MQ=255)
                                                                          ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                 >  4:409125/1‑98 (MQ=255)
                                                                          ttACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                 <  3:409125/98‑1 (MQ=255)
                                                                               aTGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAgg                                                                                                                                     >  3:978833/1‑72 (MQ=255)
                                                                               aTGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGAACAGGCAgg                                                                                                                                     <  4:978833/73‑2 (MQ=255)
                                                                                             cccATTTACTATGACCTGCCATAAACATATCTCCCGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                     >  3:374332/1‑91 (MQ=255)
                                                                                             cccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGt                                                                                                     <  4:374332/91‑1 (MQ=255)
                                                                                             cccATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCa                                            >  2:1078423/1‑148 (MQ=255)
                                                                                               cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                 >  1:1039879/1‑77 (MQ=255)
                                                                                               cATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCaa                                                                                                                 <  2:1039879/77‑1 (MQ=255)
                                                                                                      tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa                                                                                                                          <  4:938626/61‑1 (MQ=255)
                                                                                                      tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa                                                                                                                          >  3:938626/1‑61 (MQ=255)
                                                                                                      tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAgg                                                                                            >  3:831385/1‑91 (MQ=255)
                                                                                                      tATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAgg                                                                                            <  4:831385/91‑1 (MQ=255)
                                                                                                       aTGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcagca                                                                     >  4:118664/1‑113 (MQ=255)
                                                                                                       aTGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCgcagca                                                                     <  3:118664/113‑1 (MQ=255)
                                                                                                          aCCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGaa                               >  4:262491/1‑148 (MQ=255)
                                                                                                           ccTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa                                                                                                                          <  3:1075871/56‑1 (MQ=255)
                                                                                                           ccTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACaa                                                                                                                          >  4:1075871/1‑56 (MQ=255)
                                                                                                                   aaaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     <  4:733992/53‑1 (MQ=255)
                                                                                                                   aaaaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGt                                                                                                                     >  3:733992/1‑53 (MQ=255)
                                                                                                                     aaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACtt                                                                                        >  4:359821/1‑80 (MQ=255)
                                                                                                                     aaaTATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACtt                                                                                        <  3:359821/80‑1 (MQ=255)
                                                                                                                       atatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCa                                                                                                                  >  2:333766/1‑52 (MQ=255)
                                                                                                                       atatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCa                                                                                                                  <  1:333766/52‑1 (MQ=255)
                                                                                                                        tatCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCt                 <  3:262491/148‑1 (MQ=255)
                                                                                                                               aGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTc                                                                                                >  4:607754/1‑62 (MQ=255)
                                                                                                                               aGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTc                                                                                                <  3:607754/62‑1 (MQ=255)
                                                                                                                                       ctgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCaa  <  1:1078423/148‑1 (MQ=255)
                                                                                                                                        tgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGt              >  2:351937/1‑135 (MQ=255)
                                                                                                                                        tgcctgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGt              <  1:351937/135‑1 (MQ=255)
                                                                                                                                            tgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcg     <  4:1010270/140‑1 (MQ=255)
                                                                                                                                            tgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcg     >  1:395931/1‑140 (MQ=255)
                                                                                                                                            tgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcg     <  2:395931/140‑1 (MQ=255)
                                                                                                                                            tgTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAgcg     >  3:1010270/1‑140 (MQ=255)
                                                                                                                                              ttCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGt                                       <  2:1026725/104‑1 (MQ=255)
                                                                                                                                              ttCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGt                                       >  1:1026725/1‑104 (MQ=255)
                                                                                                                                               tCAGGCAGCGTTAATTACAAACTGTTCAATCTCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGt                                       >  4:975915/1‑103 (MQ=255)
                                                                                                                                               tCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGTCGCATCAAGCCACTTGTAAGCCAGATGt                                       <  3:975915/103‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                           
CCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAATTACAAACTGTTCAATCGCCTGCCGGTTGCTCCAGGACTTAGTGAGCGCCGCCGCAGCAGGCGCATCAAGCCACTTGTAAGCCAGATGTTCAGTGAAAACGATCTGCCGCTCGTGCGGAAGCGCAA  >  NC_000913/1948037‑1948319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: