Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,292 C→G 7.2% A77A (GCG→GCC aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,2920CG 7.2% 191.6 / 3.7 54A77A (GCG→GCCaspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base G (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAT  >  NC_000913/1950155‑1950437
                                                                                                                                         |                                                                                                                                                  
ttCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACgg                                                                                                                                         >  3:17124/1‑148 (MQ=255)
  cAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTg                                                                                                                                       <  3:890017/148‑1 (MQ=255)
      cgcgCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCg                                                                                                                                   <  4:17124/148‑1 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 <  1:450256/148‑1 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 >  2:908515/1‑148 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 >  2:450256/1‑148 (MQ=255)
        cgcTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 <  1:908515/148‑1 (MQ=255)
            tcttcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACg                                                                                                                                          <  1:362107/135‑1 (MQ=255)
            tcttcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACg                                                                                                                                          >  2:362107/1‑135 (MQ=255)
               tcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 <  1:227920/141‑1 (MQ=255)
               tcGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 >  2:227920/1‑141 (MQ=255)
                             gTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             <  2:958277/147‑1 (MQ=255)
                             gTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             >  1:958277/1‑147 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTa                                                                                                                                            <  1:118705/114‑1 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTa                                                                                                                                            >  2:118705/1‑114 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGc                                                                                                                       >  2:650133/1‑135 (MQ=255)
                               tAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGc                                                                                                                       <  1:650133/135‑1 (MQ=255)
                                                    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGaa                                                                                                                          <  1:480160/111‑1 (MQ=255)
                                                    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGaa                                                                                                                          >  2:480160/1‑111 (MQ=255)
                                                               atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             <  1:35180/113‑1 (MQ=255)
                                                               atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCAtt                                                                                                             >  2:35180/1‑113 (MQ=255)
                                                                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCg                                                                                                                                   <  4:569453/83‑1 (MQ=255)
                                                                       cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCg                                                                                                                                   >  3:569453/1‑83 (MQ=255)
                                                                        aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                                                                 >  3:1092208/1‑148 (MQ=255)
                                                                        aGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                                                                 >  3:769980/1‑148 (MQ=255)
                                                                                 gCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        <  4:1092208/148‑1 (MQ=255)
                                                                                    aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 <  2:452456/72‑1 (MQ=255)
                                                                                    aGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTg                                                                                                                                 >  1:452456/1‑72 (MQ=255)
                                                                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTa                                                                                                                                            <  2:636766/56‑1 (MQ=255)
                                                                                         ttCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTa                                                                                                                                            >  1:636766/1‑56 (MQ=255)
                                                                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCag                                                                                                  >  3:185188/1‑97 (MQ=255)
                                                                                          tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCag                                                                                                  <  4:185188/97‑1 (MQ=255)
                                                                                           cGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGAt                                              >  1:224685/1‑148 (MQ=255)
                                                                                               ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAcc                                                                                                                              >  3:357628/1‑64 (MQ=255)
                                                                                               ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGAcc                                                                                                                              <  4:357628/64‑1 (MQ=255)
                                                                                                  accGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTcg                                       >  4:870165/2‑148 (MQ=255)
                                                                                                      gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                                                 >  1:1017066/1‑102 (MQ=255)
                                                                                                      gTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                                                                 <  2:1017066/102‑1 (MQ=255)
                                                                                                             tatCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCat                            <  2:224685/148‑1 (MQ=255)
                                                                                                                cgcgGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                                                                     >  2:676551/1‑88 (MQ=255)
                                                                                                                cgcgGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTaa                                                                                     <  1:676551/88‑1 (MQ=255)
                                                                                                                      tAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGaa                   <  4:769980/148‑1 (MQ=255)
                                                                                                                            ttttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        <  2:29244/105‑1 (MQ=255)
                                                                                                                            ttttttCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                                                        >  1:29244/1‑105 (MQ=255)
                                                                                                                                    tCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATc                         <  4:889016/128‑1 (MQ=255)
                                                                                                                                    tCACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATc                         >  3:889016/1‑128 (MQ=255)
                                                                                                                                     cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                                                                      >  3:717135/1‑82 (MQ=37)
                                                                                                                                     cACGGGCACCGTACGTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                                                                      <  4:717135/82‑1 (MQ=37)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCATAGGCCAGCTTTa                                                                                      <  4:736468/66‑1 (MQ=255)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                                                      >  3:736468/1‑66 (MQ=255)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAATTACCTGCACg                                                <  3:668339/104‑1 (MQ=255)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                                                      <  4:400306/98‑1 (MQ=255)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                                                      >  3:400306/1‑98 (MQ=255)
                                                                                                                                     cACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACg                                                >  4:668339/1‑104 (MQ=255)
                                                                                                                                       cgcgCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAt  >  2:377113/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
TTCAGACGCGCTTCTTCGGTGTTGACGTGGTTAGAGTCAAGCGGCAGAACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCACGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTCGCGCATATCGATGAAGATCAGGCTACCAAGAT  >  NC_000913/1950155‑1950437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: