Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,986,176 A→T 8.5% intergenic (‑48/‑749) araF ← / → ftnB L‑arabinose ABC transporter periplasmic binding protein/ferritin B, putative ferrous iron reservoir

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,986,1760AT 8.5% 159.8 / 5.8 47intergenic (‑48/‑749)araF/ftnBL‑arabinose ABC transporter periplasmic binding protein/ferritin B, putative ferrous iron reservoir
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base T (2/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TCACCAGAAAACCGAGCTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCAT  >  NC_000913/1986032‑1986315
                                                                                                                                                |                                                                                                                                           
tCACCAGAAAACCGAGCTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACAt                                                                                                                                          >  3:194367/1‑148 (MQ=255)
 cACCAGAAAACCGAGCTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACAtt                                                                                                                                         <  4:194367/148‑1 (MQ=255)
      gAAAACCGAGCTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTa                                                                                                                                    >  3:214576/1‑148 (MQ=255)
                cTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCgg                                                                                                                          <  4:214576/148‑1 (MQ=255)
                  tCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGaa                                                                                                                        >  2:189968/1‑148 (MQ=255)
                           ccGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCa                                                                                                               >  2:643008/1‑148 (MQ=255)
                                  aGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCgg                                                                                                                          >  3:370679/1‑130 (MQ=255)
                                  aGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCgg                                                                                                                          <  4:370679/130‑1 (MQ=255)
                                   gCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       <  1:643008/148‑1 (MQ=255)
                                   gCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       <  1:189968/148‑1 (MQ=255)
                                                tAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAAc                                                                                                   >  1:709690/1‑139 (MQ=255)
                                                tAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAAc                                                                                                   <  2:709690/139‑1 (MQ=255)
                                                          cAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGTCATTCGtt                                                                                                                                     <  2:336757/95‑1 (MQ=255)
                                                          cAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGTCATTCGtt                                                                                                                                     >  1:336757/1‑95 (MQ=255)
                                                                           gggCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCtt                                                                                                        >  1:460422/1‑107 (MQ=255)
                                                                           gggCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCtt                                                                                                        <  2:460422/107‑1 (MQ=255)
                                                                               tttAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAg                                                                       <  2:649745/136‑1 (MQ=255)
                                                                               tttAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAg                                                                       >  1:649745/1‑136 (MQ=255)
                                                                                ttAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACtgt                                                                                 >  1:903353/1‑125 (MQ=255)
                                                                                ttAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACtgt                                                                                 <  2:903353/125‑1 (MQ=255)
                                                                                    tAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATaa                                                                                         <  1:999416/113‑1 (MQ=255)
                                                                                    tAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATaa                                                                                         >  2:999416/1‑113 (MQ=255)
                                                                                     aaaTTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAaattaat                                                     >  3:492275/1‑148 (MQ=255)
                                                                                        tttGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGTCATTCGtttt                                                                                                                                   <  3:963140/67‑3 (MQ=255)
                                                                                        tttGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGTCATTCGtttt                                                                                                                                   >  4:963140/1‑65 (MQ=255)
                                                                                        tttGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCga                                                              >  3:779608/1‑136 (MQ=255)
                                                                                        tttGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCga                                                              <  4:779608/136‑1 (MQ=255)
                                                                                        tttGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATtct                                                  <  4:492275/148‑1 (MQ=255)
                                                                                                  gTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTaaa                                                                                               >  4:447503/1‑93 (MQ=255)
                                                                                                  gTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTaaa                                                                                               <  3:447503/93‑1 (MQ=255)
                                                                                                         ccAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       >  3:417100/1‑78 (MQ=255)
                                                                                                         ccAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       <  4:417100/78‑1 (MQ=255)
                                                                                                          cAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTgtgt                                                                               <  2:242045/101‑1 (MQ=255)
                                                                                                          cAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTgtgt                                                                               >  1:242045/1‑101 (MQ=255)
                                                                                                           aGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       <  4:989950/76‑1 (MQ=255)
                                                                                                           aGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCttt                                                                                                       >  3:989950/1‑76 (MQ=255)
                                                                                                            gCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCg                                                               >  3:513841/1‑115 (MQ=255)
                                                                                                            gCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCg                                                               <  4:513841/115‑1 (MQ=255)
                                                                                                              tttAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTgtgt                                                                               <  2:501418/97‑1 (MQ=255)
                                                                                                              tttAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTgtgt                                                                               >  1:501418/1‑97 (MQ=255)
                                                                                                                     tCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGc                         <  2:564592/144‑1 (MQ=255)
                                                                                                                     tCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGc                         >  1:564592/1‑144 (MQ=255)
                                                                                                                      cGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAAt                    >  1:879440/1‑148 (MQ=255)
                                                                                                                        ttttGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTa                  <  2:879440/148‑1 (MQ=255)
                                                                                                                                        aaCGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAaattaat                                                     <  3:396286/97‑1 (MQ=255)
                                                                                                                                        aaCGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAaattaat                                                     >  4:396286/1‑97 (MQ=255)
                                                                                                                                        aaCGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCAt  >  2:9121/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                           
TCACCAGAAAACCGAGCTTCAGGTTCTCCGCCATAGCGGATTGTGACATAACGGCTGCCAGACCAATGGCTGCCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCAT  >  NC_000913/1986032‑1986315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑