| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,991,866 | T→A | 8.0% | noncoding (36/87 nt) | leuZ ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,991,866 | 0 | T | A | 8.0% | 254.5 / 11.2 | 75 | noncoding (36/87 nt) | leuZ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base T (35/34); new base A (3/3); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01 | |||||||||||
AAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTC > NC_000913/1991749‑1992005 | aaaaaaCCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATc > 4:624656/1‑148 (MQ=255) gAAGGTGGTTTCACGACACTGCTTATTCCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGga < 1:964165/147‑1 (MQ=255) gAAGGTGGTTTCACGACACTGCTTATTCCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGga > 2:964165/1‑147 (MQ=255) tggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATccc < 4:905951/108‑1 (MQ=255) tggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATccc > 3:905951/1‑108 (MQ=255) tggtTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCTGGCTCGGGAAGAAAg > 2:382940/1‑148 (MQ=255) ttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa > 2:928871/1‑148 (MQ=255) ttCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGa < 1:382940/148‑1 (MQ=255) tCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAg > 1:244132/1‑148 (MQ=255) cACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAgg < 1:928871/148‑1 (MQ=255) cGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATcc > 3:828744/1‑99 (MQ=255) cGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATcc < 4:828744/99‑1 (MQ=255) acacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGaaa > 3:749579/1‑137 (MQ=255) acacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGaaa < 4:749579/137‑1 (MQ=255) acacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtcg > 4:104164/1‑99 (MQ=255) acacTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtcg < 3:104164/101‑3 (MQ=255) cTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCggg < 1:1004055/122‑1 (MQ=255) cTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCggg > 2:1004055/1‑122 (MQ=255) tggttATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTcc > 3:505225/4‑148 (MQ=255) cTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCgg < 3:624656/148‑1 (MQ=255) tATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCtt > 3:796600/1‑92 (MQ=255) tATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCtt < 4:796600/92‑1 (MQ=255) tATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAg > 2:44990/1‑148 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATcc > 3:630228/1‑82 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATcc < 4:630228/82‑1 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCt < 3:321859/91‑1 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCt > 4:321859/1‑91 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGTTTCCACCATc < 1:593953/106‑1 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATc > 2:593953/1‑106 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt > 3:169046/1‑124 (MQ=255) ttGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGt < 4:169046/124‑1 (MQ=255) gATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg < 2:244132/148‑1 (MQ=255) gATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGACTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg < 4:505225/148‑1 (MQ=255) tATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAg < 1:44990/148‑1 (MQ=255) cTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg > 1:180060/1‑115 (MQ=255) cTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg < 2:180060/115‑1 (MQ=255) cTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtc > 3:915966/1‑73 (MQ=255) cTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATCCCtc < 4:915966/74‑2 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCg < 4:602895/101‑1 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCg > 3:602895/1‑101 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCg < 2:553176/125‑1 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCg > 1:553176/1‑125 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATccc < 4:1010722/70‑1 (MQ=255) tATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTAAAATccc > 3:1010722/1‑70 (MQ=255) cTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTCAATCCCTTGTGTCTACCGTTTCCACCAACCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGaa < 2:439257/130‑1 (MQ=255) cTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGaa > 1:439257/1‑130 (MQ=255) tCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCaa > 4:503693/1‑148 (MQ=255) aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCt > 3:956730/1‑61 (MQ=255) aTGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCt < 4:956730/61‑1 (MQ=255) tACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGcgcg < 1:664411/106‑1 (MQ=255) tACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGcgcg > 2:664411/1‑106 (MQ=255) ggAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAg > 2:252283/1‑123 (MQ=255) ggAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAg < 1:252283/123‑1 (MQ=255) aCTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtg > 1:206921/1‑46 (MQ=255) aCTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTtg < 2:206921/46‑1 (MQ=255) ttGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTa < 3:503693/148‑1 (MQ=255) tGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaag < 2:214768/76‑1 (MQ=255) tGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGgaag > 1:214768/1‑76 (MQ=255) cccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAg < 1:983030/76‑1 (MQ=255) cccGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAg > 2:983030/1‑76 (MQ=255) cGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa > 2:260708/1‑117 (MQ=255) cGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCa < 1:260708/117‑1 (MQ=255) cacaGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGAc < 4:90697/101‑1 (MQ=255) cacaGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGAc > 3:90697/1‑101 (MQ=255) caGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCg > 3:165094/1‑118 (MQ=255) caGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCg < 4:165094/118‑1 (MQ=255) cgAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTc > 2:491569/1‑55 (MQ=255) cgAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTc < 1:491569/55‑1 (MQ=255) aGGGATTTTAAATCCCTTGTGTCTACCGATTCCACAATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCtttt > 1:343977/1‑147 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg > 2:204089/1‑56 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTgg < 1:204089/56‑1 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg < 4:28135/80‑1 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg > 3:28135/1‑80 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg < 2:3801/80‑1 (MQ=255) gATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCgg > 1:3801/1‑80 (MQ=255) tttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAgg < 2:792965/56‑1 (MQ=255) tttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAgg > 1:792965/1‑56 (MQ=255) tttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt < 2:1055285/112‑1 (MQ=255) tttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCtt > 1:1055285/1‑112 (MQ=255) ttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcg > 2:991785/1‑119 (MQ=255) ttAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAAcg < 1:991785/119‑1 (MQ=255) | AAAAAACCACCCGAAGGTGGTTTCACGACACTGCTTATTGCTTTGATTTTATTCTTATCTTTCCCATGGTACCCGGAGCGGGACTTGAACCCGCACAGCGCGAACGCCGAGGGATTTTAAATCCCTTGTGTCTACCGATTCCACCATCCGGGCTCGGGAAGAAAGTGGAGGCGCGTTCCGGAGTCGAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTC > NC_000913/1991749‑1992005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |