Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,020,841 G→A 5.5% R252H (CGC→CAC)  fliM → flagellar motor switching and energizing component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,020,8410GA 5.5% 245.0 / 4.3 73R252H (CGC→CAC) fliMflagellar motor switching and energizing component
Reads supporting (aligned to +/- strand):  ref base G (35/34);  new base A (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

TGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTC  >  NC_000913/2020703‑2020983
                                                                                                                                          |                                                                                                                                              
tgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCa                                                                                                                                             >  2:648100/1‑142 (MQ=255)
tgGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCa                                                                                                                                             <  1:648100/142‑1 (MQ=255)
 ggAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgcag                                                                                                                                      >  4:576994/1‑148 (MQ=255)
  gagaTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgcagc                                                                                                                                     >  4:930541/1‑148 (MQ=255)
     aTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   <  3:580608/147‑1 (MQ=255)
     aTTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   >  4:580608/1‑147 (MQ=255)
      ttGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTc                                                                                                                                 <  1:33117/148‑1 (MQ=255)
               tGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGt                                                                                                                                          <  3:909280/130‑1 (MQ=255)
               tGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGt                                                                                                                                          >  4:909280/1‑130 (MQ=255)
                  ccGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagct                                                                                                                     <  3:930541/148‑1 (MQ=255)
                     gCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGt                                                                                                                  <  3:576994/148‑1 (MQ=255)
                        aaTTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagct                                                                                                                     >  3:421892/1‑142 (MQ=255)
                        aaTTTAATATCTGCCTGCCATCCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACagctggagct                                                                                                                     <  4:421892/142‑1 (MQ=255)
                                atCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTg                                                                                                       >  4:478902/1‑148 (MQ=255)
                                atCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGt                                                                                                                                          <  2:744294/113‑1 (MQ=255)
                                atCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGCGCACCAGGt                                                                                                                                          >  1:744294/1‑113 (MQ=255)
                                  ctgcctgcCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGt                                                                                                                  <  2:707474/135‑1 (MQ=255)
                                  ctgcctgcCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGt                                                                                                                  >  1:707474/1‑135 (MQ=255)
                                        gccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACtt                                                                                                         <  4:352177/138‑1 (MQ=255)
                                        gccATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACtt                                                                                                         >  3:352177/1‑138 (MQ=255)
                                           aTTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGGACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAAc                                                                                                           <  4:828499/133‑1 (MQ=255)
                                           aTTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGGACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAAc                                                                                                           >  3:828499/1‑133 (MQ=255)
                                           aTTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCg                                                                                            >  2:1053805/1‑148 (MQ=255)
                                            ttCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATAtctc                                                                                             >  2:11969/1‑146 (MQ=255)
                                            ttCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATAtctc                                                                                             <  1:11969/146‑1 (MQ=255)
                                             tCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCt                                                                                          <  3:478902/148‑1 (MQ=255)
                                                       cGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCg                                                                                >  2:956390/1‑148 (MQ=255)
                                                           ccGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGa                                                                            >  1:278977/1‑148 (MQ=255)
                                                                    gAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAAc                                                                   <  2:278977/148‑1 (MQ=255)
                                                                    gAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAAc                                                                   <  1:1053805/148‑1 (MQ=255)
                                                                     aaTTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGata                                                                                                 <  3:841272/117‑1 (MQ=255)
                                                                     aaTTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGata                                                                                                 >  4:841272/1‑117 (MQ=255)
                                                                      aTTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgc                                                                                                                                        <  3:735648/77‑1 (MQ=255)
                                                                      aTTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTgc                                                                                                                                        >  4:735648/1‑77 (MQ=255)
                                                                           tgGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   >  2:36460/1‑77 (MQ=255)
                                                                           tgGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   <  1:36460/77‑1 (MQ=255)
                                                                              ttAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCgg                                                         >  4:656101/1‑148 (MQ=255)
                                                                               tAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAAc                                                                   <  4:576749/137‑1 (MQ=255)
                                                                               tAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAAc                                                                   >  3:576749/1‑137 (MQ=255)
                                                                                  cccGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   <  4:1109844/70‑1 (MQ=255)
                                                                                  cccGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCAt                                                                                                                                   >  3:1109844/1‑70 (MQ=255)
                                                                                     gccgcTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTc                                                  <  1:956390/148‑1 (MQ=255)
                                                                                          tGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGcc                                             <  3:656101/148‑1 (MQ=255)
                                                                                           ggAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGat                                                                                                  <  4:48878/94‑1 (MQ=255)
                                                                                           ggAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGat                                                                                                  >  3:48878/1‑94 (MQ=255)
                                                                                                   cgcgTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGcc                                             <  3:981293/139‑1 (MQ=255)
                                                                                                   cgcgTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGcc                                             >  4:981293/1‑139 (MQ=255)
                                                                                                          tGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTaaa                                                                     >  2:521221/1‑108 (MQ=255)
                                                                                                          tGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTaaa                                                                     <  1:521221/108‑1 (MQ=255)
                                                                                                          tGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTc                                                  >  1:1068920/1‑127 (MQ=255)
                                                                                                          tGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTc                                                  <  2:1068920/127‑1 (MQ=255)
                                                                                                              gATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGaaaaa                                   >  2:891516/1‑138 (MQ=255)
                                                                                                              gATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGaaaaa                                   <  1:891516/138‑1 (MQ=255)
                                                                                                                tCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAtt                                                                          >  3:353732/1‑97 (MQ=255)
                                                                                                                tCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAtt                                                                          <  4:353732/97‑1 (MQ=255)
                                                                                                                 cAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGa                              >  1:133329/1‑140 (MQ=255)
                                                                                                                 cAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGa                              <  2:133329/140‑1 (MQ=255)
                                                                                                                   gAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACg                                                    >  1:4461/1‑116 (MQ=255)
                                                                                                                   gAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACg                                                    <  2:4461/116‑1 (MQ=255)
                                                                                                                         gcgcGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAGAACTGAACCCCGGCGACGTcc                                                 >  1:848464/1‑113 (MQ=255)
                                                                                                                         gcgcGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTcc                                                 <  2:848464/113‑1 (MQ=255)
                                                                                                                              aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAt                                                                           <  3:1134435/82‑1 (MQ=255)
                                                                                                                              aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAt                                                                           <  3:10066/82‑1 (MQ=255)
                                                                                                                              aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAt                                                                           >  4:10066/1‑82 (MQ=255)
                                                                                                                              aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGAt                                                                           >  4:1134435/1‑82 (MQ=255)
                                                                                                                              aTAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGa         >  1:1070729/1‑148 (MQ=255)
                                                                                                                                aaCCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATg             <  1:711086/142‑1 (MQ=255)
                                                                                                                                aaCCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATg             >  2:711086/1‑142 (MQ=255)
                                                                                                                                  ccTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAAc                                                              >  2:151380/1‑91 (MQ=255)
                                                                                                                                  ccTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAAc                                                              <  1:151380/91‑1 (MQ=255)
                                                                                                                                     ggTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTg                                               <  1:603525/103‑1 (MQ=255)
                                                                                                                                     ggTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTg                                               >  2:603525/1‑103 (MQ=255)
                                                                                                                                     ggTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTc  >  2:504945/1‑148 (MQ=255)
                                                                                                                                         cgcCAGGTGCAGCACTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGaaaa                                    >  4:467840/1‑110 (MQ=255)
                                                                                                                                         cgcCAGGTGCAGCACTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGaaaa                                    <  3:467840/110‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
TGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTACGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGTAATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAGCTGGTCGCCAACTTTGCCGATATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAACCCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCGCCCATGTTGACGGCGTC  >  NC_000913/2020703‑2020983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: