| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,041,159 | G→A | 8.0% | intergenic (+41/‑216) | yedZ → / → zinT | inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,041,159 | 0 | G | A | 8.0% | 157.0 / 4.8 | 50 | intergenic (+41/‑216) | yedZ/zinT | inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic |
| Reads supporting (aligned to +/- strand): ref base G (23/23); new base A (2/2); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
TTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAA > NC_000913/2041047‑2041280 | ttACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGcgcg > 4:450785/1‑120 (MQ=255)ttACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGcgcg < 3:450785/120‑1 (MQ=255) agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc > 4:391289/1‑115 (MQ=255) agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc < 1:742971/115‑1 (MQ=255) agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc < 3:391289/115‑1 (MQ=255) agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc > 2:742971/1‑115 (MQ=255) gaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGgcg > 4:265880/1‑107 (MQ=255) gaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGgcg < 3:265880/107‑1 (MQ=255) tAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTAt < 1:683984/101‑1 (MQ=255) tAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTAt > 2:683984/1‑101 (MQ=255) ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa > 1:240872/1‑87 (MQ=255) ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa < 4:1080136/87‑1 (MQ=255) ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa < 2:240872/87‑1 (MQ=255) ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa > 3:1080136/1‑87 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCGCTGCCGGATGTGgcgct > 3:379005/1‑83 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGgcgct < 4:379005/84‑2 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg < 2:994508/128‑1 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg > 1:994508/1‑128 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg > 4:1059863/1‑132 (MQ=255) ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg < 3:1059863/132‑1 (MQ=255) tACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa < 3:694754/148‑1 (MQ=255) tACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa > 4:694754/1‑148 (MQ=255) aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAg < 3:146192/110‑1 (MQ=255) aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAg > 4:146192/1‑110 (MQ=255) aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGccacat > 2:584881/1‑83 (MQ=37) aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGccacat < 1:584881/87‑5 (MQ=37) gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt > 3:940499/1‑117 (MQ=255) gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt < 4:940499/117‑1 (MQ=255) acaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGc < 3:1034716/80‑1 (MQ=25) acaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGc > 4:1034716/1‑80 (MQ=25) aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc < 4:935932/67‑1 (MQ=255) aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc > 3:935932/1‑67 (MQ=255) aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAAt < 3:869515/125‑1 (MQ=255) aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAAt > 4:869515/1‑125 (MQ=255) tATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa > 1:104967/1‑96 (MQ=255) tATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa < 2:104967/96‑1 (MQ=255) tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt < 1:934228/95‑1 (MQ=255) tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt > 2:934228/1‑95 (MQ=255) tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca < 3:501401/108‑1 (MQ=255) tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca > 4:501401/1‑108 (MQ=255) ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtcatca < 1:672825/101‑1 (MQ=255) ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtcatca > 2:672825/1‑101 (MQ=255) tCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAg > 1:256852/1‑148 (MQ=255) tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGt < 4:337859/78‑1 (MQ=255) tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGt > 3:337859/1‑78 (MQ=255) cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGccc > 3:589956/1‑44 (MQ=255) cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGccc < 4:589956/44‑1 (MQ=255) cAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGaa < 2:256852/148‑1 (MQ=255) tCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt < 4:1007915/67‑1 (MQ=255) tCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt > 3:1007915/1‑67 (MQ=255) | TTACGGTATAAGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAA > NC_000913/2041047‑2041280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |