Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,041,167 A→C 10.0% intergenic (+49/‑208) yedZ → / → zinT inner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,041,1670AC10.0% 128.9 / 6.0 40intergenic (+49/‑208)yedZ/zinTinner membrane heme subunit for periplasmic YedYZ reductase/zinc and cadmium binding protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (18/18);  new base C (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

AGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAA  >  NC_000913/2041057‑2041280
                                                                                                              |                                                                                                                 
agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               >  2:742971/1‑115 (MQ=255)
agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               >  4:391289/1‑115 (MQ=255)
agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               <  1:742971/115‑1 (MQ=255)
agaagTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               <  3:391289/115‑1 (MQ=255)
                   tAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTAt                                                                                                          >  2:683984/1‑101 (MQ=255)
                   tAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTAt                                                                                                          <  1:683984/101‑1 (MQ=255)
                        ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa                                                                                                                   >  1:240872/1‑87 (MQ=255)
                        ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa                                                                                                                   <  4:1080136/87‑1 (MQ=255)
                        ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa                                                                                                                   >  3:1080136/1‑87 (MQ=255)
                        ggTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGa                                                                                                                   <  2:240872/87‑1 (MQ=255)
                          ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg                                                                        <  2:994508/128‑1 (MQ=255)
                          ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCg                                                                        >  1:994508/1‑128 (MQ=255)
                          ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg                                                                    >  4:1059863/1‑132 (MQ=255)
                          ttACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTg                                                                    <  3:1059863/132‑1 (MQ=255)
                           tACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                   <  3:694754/148‑1 (MQ=255)
                           tACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCa                                                   >  4:694754/1‑148 (MQ=255)
                                 aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAg                                                                                   >  4:146192/1‑110 (MQ=255)
                                 aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAg                                                                                   <  3:146192/110‑1 (MQ=255)
                                 aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGccacat                                                                                                          <  1:584881/87‑5 (MQ=37)
                                 aGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGccacat                                                                                                          >  2:584881/1‑83 (MQ=37)
                                      gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt                                                                       <  4:940499/117‑1 (MQ=255)
                                      gTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGt                                                                       >  3:940499/1‑117 (MQ=255)
                                             acaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGc                                                                                                     <  3:1034716/80‑1 (MQ=25)
                                             acaaATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATAGGGCGCTCGCGCCACATCCGGc                                                                                                     >  4:1034716/1‑80 (MQ=25)
                                                aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               <  4:935932/67‑1 (MQ=255)
                                                aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAgcgc                                                                                                               >  3:935932/1‑67 (MQ=255)
                                                aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAAt                                                     >  4:869515/1‑125 (MQ=255)
                                                aaTTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAAt                                                     <  3:869515/125‑1 (MQ=255)
                                                   tATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                               >  1:104967/1‑96 (MQ=255)
                                                   tATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTa                                                                               <  2:104967/96‑1 (MQ=255)
                                                     tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                              >  2:934228/1‑95 (MQ=255)
                                                     tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTAt                                                                              <  1:934228/95‑1 (MQ=255)
                                                     tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca                                                                 <  3:501401/108‑1 (MQ=255)
                                                     tCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtca                                                                 >  4:501401/1‑108 (MQ=255)
                                                               ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtcatca                                                              >  2:672825/1‑101 (MQ=255)
                                                               ttCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGtcatca                                                              <  1:672825/101‑1 (MQ=255)
                                                                tCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAg              >  1:256852/1‑148 (MQ=255)
                                                                   tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGt                                                                                 <  4:337859/78‑1 (MQ=255)
                                                                   tatTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGt                                                                                 >  3:337859/1‑78 (MQ=255)
                                                                         cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGccc                                                                                                             <  4:589956/44‑1 (MQ=255)
                                                                         cGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGccc                                                                                                             >  3:589956/1‑44 (MQ=255)
                                                                            cAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGaa  <  2:256852/148‑1 (MQ=255)
                                                                                          tCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt                                                                     <  4:1007915/67‑1 (MQ=255)
                                                                                          tCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGttt                                                                     >  3:1007915/1‑67 (MQ=255)
                                                                                                              |                                                                                                                 
AGAAGTTGCGTTCCCTTTTTAACCGGTTACGCAAGCAGGTTCACAACAAATTATCTGTATAACTTCATATTGCCGACAAAGTACGCGTTATCCCTGCCGGATGTGGCGCGAGCGCCCTATCCGGCATACACAGATCGCATCAGGTTATGTTTCGTTTGTCATCAACCTCAAATCACCAAAATAATTTATTTTATTTCAATAAGATAGATAAGTAGAACTGAGAA  >  NC_000913/2041057‑2041280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: