Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,130,803 G→T 5.1% Q26K (CAG→AAG)  wcaD ← putative colanic acid polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,130,8030GT 5.1% 281.6 / 5.0 78Q26K (CAG→AAG) wcaDputative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base G (37/37);  new base T (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

ACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAA  >  NC_000913/2130660‑2130950
                                                                                                                                               |                                                                                                                                                   
aCCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCg                                                                                                                                                 >  4:711738/1‑148 (MQ=255)
         aTAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACg                                                                                                                                        >  4:175541/1‑148 (MQ=255)
             ttAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTg                                                                                                                                                     >  4:767964/1‑131 (MQ=255)
             ttAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTg                                                                                                                                                     <  3:767964/131‑1 (MQ=255)
                  cGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagc                                                                                                                               <  3:175541/148‑1 (MQ=255)
                  cGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagc                                                                                                                               <  3:711738/148‑1 (MQ=255)
                      gTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGAt                                                                                                                           >  3:1022244/1‑148 (MQ=255)
                             ccGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAg                                                                                                                    <  4:1022244/148‑1 (MQ=255)
                              cgcgCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCCAGCTTGGCGaa                                                                                                                                               <  4:520002/120‑2 (MQ=255)
                              cgcgCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCCAGCTTGGCGaa                                                                                                                                               >  3:520002/1‑119 (MQ=255)
                              cgcgCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTggg                                                                                                                                                   >  2:746014/1‑116 (MQ=255)
                              cgcgCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTggg                                                                                                                                                   <  1:746014/116‑1 (MQ=255)
                                  ccAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                                                                                                                                           <  3:112657/120‑1 (MQ=255)
                                  ccAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                                                                                                                                           >  4:112657/1‑120 (MQ=255)
                                               cAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagcag                                                                                                                             <  2:685489/121‑1 (MQ=255)
                                               cAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagcag                                                                                                                             >  1:685489/1‑121 (MQ=255)
                                                     tcaATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagc                                                                                                                               >  4:175161/1‑113 (MQ=255)
                                                     tcaATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagc                                                                                                                               <  3:175161/113‑1 (MQ=255)
                                                        aTTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                                                                                                                                               >  1:486522/1‑94 (MQ=255)
                                                        aTTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                                                                                                                                               <  2:486522/94‑1 (MQ=255)
                                                          tttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAc                                                                                                                                   <  1:51259/104‑1 (MQ=255)
                                                          tttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAc                                                                                                                                   >  2:51259/1‑104 (MQ=255)
                                                           ttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGc                                                                                                   >  4:1145707/1‑135 (MQ=255)
                                                           ttttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGc                                                                                                   <  3:1145707/135‑1 (MQ=255)
                                                                aCGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTaa                                                                                                                                    >  2:304953/1‑97 (MQ=255)
                                                                aCGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTaa                                                                                                                                    <  1:304953/97‑1 (MQ=255)
                                                                 cGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACa                                                                                                         <  2:420106/123‑1 (MQ=255)
                                                                 cGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACa                                                                                                         >  1:420106/1‑123 (MQ=255)
                                                                   cTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTg                                                                              >  2:772333/1‑148 (MQ=255)
                                                                       tGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCCAGCTTGGCGaa                                                                                                                                               <  1:195031/79‑2 (MQ=255)
                                                                       tGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCCAGCTTGGCGaa                                                                                                                                               >  2:195031/1‑78 (MQ=255)
                                                                                aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                                                                                                                                           <  2:626083/74‑1 (MQ=255)
                                                                                aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                                                                                                                                           >  1:626083/1‑74 (MQ=255)
                                                                                 aaaCAGCAGTGACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGa                                                                                                                                                >  1:592829/1‑68 (MQ=255)
                                                                                 aaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGa                                                                                                                                                <  2:592829/68‑1 (MQ=255)
                                                                                            aCAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTc                                                     >  3:673717/1‑148 (MQ=255)
                                                                                              aaGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGt                                                                                <  1:1037076/119‑1 (MQ=255)
                                                                                              aaGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGt                                                                                >  2:1037076/1‑119 (MQ=255)
                                                                                                gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCaa                                                                                                           >  4:21879/1‑90 (MQ=255)
                                                                                                gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCaa                                                                                                           <  3:21879/90‑1 (MQ=255)
                                                                                                gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa                                                                                   <  4:986772/114‑1 (MQ=255)
                                                                                                gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa                                                                                   >  3:986772/1‑114 (MQ=255)
                                                                                                     gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATa                                                                         <  3:2552/119‑1 (MQ=255)
                                                                                                     gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATa                                                                         >  4:2552/1‑119 (MQ=255)
                                                                                                       aaaaaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGAtt                                                                                           >  4:1063298/1‑99 (MQ=255)
                                                                                                       aaaaaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGAtt                                                                                           <  3:1063298/99‑1 (MQ=255)
                                                                                                          aaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGAc                                                                            <  4:764331/111‑1 (MQ=255)
                                                                                                          aaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGAc                                                                            >  2:771121/1‑111 (MQ=255)
                                                                                                          aaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGAc                                                                            <  1:771121/111‑1 (MQ=255)
                                                                                                          aaaGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGAc                                                                            >  3:764331/1‑111 (MQ=255)
                                                                                                            aGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                          >  4:1132288/1‑127 (MQ=255)
                                                                                                            aGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                          <  3:1132288/127‑1 (MQ=255)
                                                                                                              tGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATc                                                                                                                      <  4:335597/65‑1 (MQ=255)
                                                                                                              tGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATc                                                                                                                      >  3:335597/1‑65 (MQ=255)
                                                                                                               gACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACAt                                                                          <  1:871559/108‑1 (MQ=255)
                                                                                                               gACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACAt                                                                          >  2:871559/1‑108 (MQ=255)
                                                                                                                 cGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgt                                                         >  4:910677/1‑123 (MQ=255)
                                                                                                                 cGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgt                                                         <  3:910677/123‑1 (MQ=255)
                                                                                                                     ggTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTg                            <  4:673717/148‑1 (MQ=255)
                                                                                                                      gTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTgg                           <  1:772333/148‑1 (MQ=255)
                                                                                                                            gggAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa                                                                                   >  4:1145779/1‑86 (MQ=255)
                                                                                                                            gggAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGa                                                                                   <  3:1145779/86‑1 (MQ=255)
                                                                                                                              gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTg                                                                              >  4:983307/1‑89 (MQ=255)
                                                                                                                              gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgtt                                                        >  4:152148/1‑111 (MQ=255)
                                                                                                                              gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgtt                                                        <  3:152148/111‑1 (MQ=255)
                                                                                                                              gAAACTTTCGCCAAGCTGGGCGATTTTGACCTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTg                                                                              <  3:983307/89‑1 (MQ=255)
                                                                                                                               aaaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAg                                                                                                    >  4:1018724/1‑66 (MQ=255)
                                                                                                                               aaaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAg                                                                                                    <  3:1018724/66‑1 (MQ=255)
                                                                                                                               aaaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAg                                                                                 <  1:60318/85‑1 (MQ=255)
                                                                                                                               aaaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAg                                                                                 >  2:60318/1‑85 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTg                                                                              >  1:268341/1‑83 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTg                                                                              <  2:268341/83‑1 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                      >  1:432068/1‑107 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCg                               <  2:670717/130‑1 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCg                               >  1:670717/1‑130 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGa                       <  1:31785/138‑1 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGa                       >  2:31785/1‑138 (MQ=255)
                                                                                                                                    ttCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCACCATCtgttgt                                                      <  2:432068/107‑1 (MQ=255)
                                                                                                                                         cAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCa                                                   <  4:405995/105‑1 (MQ=255)
                                                                                                                                         cAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCa                                                   >  3:405995/1‑105 (MQ=255)
                                                                                                                                           aGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATAct                                                                       <  4:1026869/83‑1 (MQ=255)
                                                                                                                                           aGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATAct                                                                       >  3:1026869/1‑83 (MQ=255)
                                                                                                                                               gggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCaa  >  2:759079/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAA  >  NC_000913/2130660‑2130950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: