Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,169,075 A→G 5.3% I122V (ATT→GTT)  yegS → phosphatidylglycerol kinase, metal‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,169,0750AG 5.3% 261.0 / 3.0 76I122V (ATT→GTT) yegSphosphatidylglycerol kinase, metal‑dependent
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base G (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

ACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATCATTCAT  >  NC_000913/2168934‑2169212
                                                                                                                                             |                                                                                                                                         
aCGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCa                                                                                                                                           >  4:37493/1‑142 (MQ=255)
aCGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCa                                                                                                                                           <  3:37493/142‑1 (MQ=255)
aCGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCga                                                                                                                                     >  4:232697/1‑148 (MQ=255)
aCGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCga                                                                                                                                     >  4:189348/1‑148 (MQ=255)
aCGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCga                                                                                                                                     >  3:1009700/1‑148 (MQ=255)
    cGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgataga                                                                                                                                 >  2:919566/1‑148 (MQ=255)
      ttGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgatagata                                                                                                                               <  3:297278/148‑1 (MQ=255)
        gATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATg                                                                                                                             <  3:189348/148‑1 (MQ=255)
            cAGTGTGAGGGGGATGACATACCCGCGCTGGGAAATTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGACAATTGCCGGTGACGCCATTGCGATAGATATGgcgc                                                                                                                         <  1:919566/148‑1 (MQ=255)
                  gAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgat                                                                                                                                    <  1:468219/131‑1 (MQ=255)
                  gAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgat                                                                                                                                    >  2:468219/1‑131 (MQ=255)
                   aGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTcaa                                                                                                                  <  3:232697/148‑1 (MQ=255)
                     ggggATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGc                                                                                                                                       >  4:977944/1‑125 (MQ=255)
                     ggggATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGc                                                                                                                                       <  3:977944/125‑1 (MQ=255)
                           gACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          <  3:525272/148‑1 (MQ=255)
                               tACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCGTTGCGATAGATATGGCGCAGGTCAACAAACAAACCTg                                                                                                      >  1:511454/1‑148 (MQ=255)
                               tACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCGTTGCGATAGATATGGCGCAGGTCAACAAACAAACCTg                                                                                                      >  3:61366/1‑148 (MQ=255)
                                aCCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATg                                                                                                                             >  2:910756/1‑124 (MQ=255)
                                aCCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATg                                                                                                                             <  1:910756/124‑1 (MQ=255)
                                 cccGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgatagata                                                                                                                               >  4:65821/1‑121 (MQ=255)
                                 cccGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCgatagata                                                                                                                               <  3:65821/121‑1 (MQ=255)
                                    gcgcTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGtt                                                                                                    >  4:624438/1‑145 (MQ=255)
                                    gcgcTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGtt                                                                                                    <  3:624438/145‑1 (MQ=255)
                                        tGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTaa                                                                                             <  4:1009700/148‑1 (MQ=255)
                                            aaTTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCGTTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATg                                                                                         <  2:511454/148‑1 (MQ=255)
                                            aaTTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCGTTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATg                                                                                         <  4:61366/148‑1 (MQ=255)
                                                     aTTAGGAACCGCCAATGACTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcg                                                                                                                          <  1:1114186/106‑1 (MQ=255)
                                                     aTTAGGAACCGCCAATGACTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGgcg                                                                                                                          >  2:1114186/1‑106 (MQ=255)
                                                      ttAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCg                                                                               >  3:1004202/1‑148 (MQ=255)
                                                         ggAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                >  3:128411/1‑128 (MQ=255)
                                                         ggAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                <  4:128411/128‑1 (MQ=255)
                                                                 cAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATgg                                                                                        >  2:863142/1‑128 (MQ=255)
                                                                 cAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATgg                                                                                        <  1:863142/128‑1 (MQ=255)
                                                                 cAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGAcg                                                                    <  4:1004202/148‑1 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          >  4:578743/1‑109 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          <  3:578743/109‑1 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          >  4:719856/1‑109 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                <  4:1116007/119‑1 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                >  3:1116007/1‑119 (MQ=255)
                                                                  aaTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCAATGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          <  3:719856/109‑1 (MQ=255)
                                                                   aTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTtta                                                                                                 <  4:935991/117‑1 (MQ=255)
                                                                   aTGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTtta                                                                                                 >  3:935991/1‑117 (MQ=255)
                                                                    tGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGatat                                                                                                                              <  3:829762/87‑1 (MQ=255)
                                                                    tGATTTGGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGatat                                                                                                                              >  4:829762/1‑87 (MQ=255)
                                                                             caccaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATCTGGGAcgcg                                                                  <  3:70103/138‑1 (MQ=255)
                                                                             caccaGTGTAGGGATTCCTGAGACACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGAcgcg                                                                  >  4:70103/1‑138 (MQ=255)
                                                                              accaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAgg                                                                                                                      <  2:522663/85‑1 (MQ=255)
                                                                              accaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAgg                                                                                                                      >  1:522663/1‑85 (MQ=255)
                                                                              accaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCaacaaac                                                                                                             >  3:642594/1‑94 (MQ=255)
                                                                              accaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCaacaaac                                                                                                             <  4:642594/94‑1 (MQ=255)
                                                                              accaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAg                                                       >  4:410720/1‑148 (MQ=255)
                                                                               ccaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGatat                                                                                                                              <  1:547878/76‑1 (MQ=255)
                                                                               ccaGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGatat                                                                                                                              >  2:547878/1‑76 (MQ=255)
                                                                                caGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGaa                                                     >  3:135685/1‑148 (MQ=255)
                                                                                   tgtAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGCGACGCCATTGCgatagat                                                                                                                                <  2:719319/70‑1 (MQ=255)
                                                                                   tgtAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGCGACGCCATTGCgatagat                                                                                                                                >  1:719319/1‑70 (MQ=255)
                                                                                     tAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTaa                                                                                             <  4:560322/103‑1 (MQ=255)
                                                                                     tAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTaa                                                                                             <  4:289716/103‑1 (MQ=255)
                                                                                     tAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTaa                                                                                             >  3:289716/1‑103 (MQ=255)
                                                                                     tAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTaa                                                                                             >  3:560322/1‑103 (MQ=255)
                                                                                       gggATTACTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTGGGGACGCGTATTACCACAGAAACGCCgg                                              >  2:929744/1‑148 (MQ=255)
                                                                                         gATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCt                                                                                                       >  1:543821/1‑89 (MQ=255)
                                                                                         gATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCt                                                                                                       <  2:543821/89‑1 (MQ=255)
                                                                                          aTTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTg                                                                                                      <  3:731142/89‑1 (MQ=255)
                                                                                          aTTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTg                                                                                                      >  4:731142/1‑89 (MQ=255)
                                                                                                gAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTaa                                     <  4:135685/148‑1 (MQ=255)
                                                                                                 aGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTaaa                                    <  1:929744/148‑1 (MQ=255)
                                                                                                      cTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          <  3:835645/73‑1 (MQ=255)
                                                                                                      cTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAAcaaacaaa                                                                                                          >  4:835645/1‑73 (MQ=255)
                                                                                                               gcgcTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                >  1:978396/1‑74 (MQ=255)
                                                                                                               gcgcTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTttat                                                                                                <  2:978396/74‑1 (MQ=255)
                                                                                                                             aaTTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTg                                                                         >  4:324689/1‑83 (MQ=255)
                                                                                                                             aaTTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTg                                                                         >  2:871873/1‑83 (MQ=255)
                                                                                                                             aaTTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTg                                                                         <  1:871873/83‑1 (MQ=255)
                                                                                                                             aaTTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTg                                                                         <  3:324689/83‑1 (MQ=255)
                                                                                                                                   cGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACAtcattcat  <  3:410720/148‑1 (MQ=255)
                                                                                                                                          gCCATTGCGATAGATATGGCGCAGGTCAACAAACAACCCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTg                          <  4:308419/117‑1 (MQ=255)
                                                                                                                                          gCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTg                          >  3:308419/1‑117 (MQ=255)
                                                                                                                                             |                                                                                                                                         
ACGGCGTTGATTCAGTGTGAGGGGGATGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATCATTCAT  >  NC_000913/2168934‑2169212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: