| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,286,142 | T→A | 12.1% | intergenic (+6/‑69) | yejM → / → proL | essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,286,142 | 0 | T | A | 12.1% | 99.7 / 6.7 | 33 | intergenic (+6/‑69) | yejM/proL | essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01 | |||||||||||
AAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT > NC_000913/2286002‑2286271 | aaGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATc < 4:704167/148‑1 (MQ=255) aCGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGGAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGt > 3:835892/1‑148 (MQ=255) aaCAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATc < 4:835892/148‑1 (MQ=255) cACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATtata < 1:660840/109‑1 (MQ=255) cACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATtata > 2:660840/1‑109 (MQ=255) tACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCa > 2:937048/1‑126 (MQ=255) tACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCa < 1:937048/126‑1 (MQ=255) tgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCg < 3:133142/123‑1 (MQ=255) tgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCg > 4:133142/1‑123 (MQ=255) aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGtt > 4:438599/1‑96 (MQ=38) aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGtt < 3:438599/96‑1 (MQ=39) gaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATc < 2:641418/86‑1 (MQ=255) gaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATc > 1:641418/1‑86 (MQ=255) gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGtt > 4:521689/1‑86 (MQ=255) gaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGtt < 3:521689/86‑1 (MQ=255) aaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa > 4:224132/1‑74 (MQ=255) aaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa < 3:224132/74‑1 (MQ=255) tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg < 4:224482/60‑1 (MQ=255) tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg < 4:1033199/60‑1 (MQ=255) tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg > 3:224482/1‑60 (MQ=255) tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAg > 3:1033199/1‑60 (MQ=255) aGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATACATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt < 1:780539/92‑1 (MQ=255) aGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt > 2:780539/1‑92 (MQ=255) gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACg < 1:715576/80‑1 (MQ=255) gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACg > 2:715576/1‑80 (MQ=255) aGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa < 2:435757/92‑1 (MQ=255) aGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa > 1:435757/1‑92 (MQ=255) cTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt > 3:665135/1‑63 (MQ=255) cTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgt < 4:665135/63‑1 (MQ=255) cTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCGTGGGGTGTCGGGGGTCGGAGGTTCAAAt > 1:869534/1‑143 (MQ=255) cTAACTGATTAATTATAAATCAGTTAGCGAAATATCTGACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCGTGGGGTGTCGGGGGTCGGAGGTTCAAAt < 2:869534/143‑1 (MQ=255) aaCTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAg < 4:572926/103‑1 (MQ=39) aaCTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAg > 3:572926/1‑103 (MQ=39) aaCTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCg > 4:121894/1‑130 (MQ=255) aaCTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCg < 3:121894/130‑1 (MQ=255) taattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCt > 1:916069/1‑91 (MQ=255) taattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCt < 2:916069/91‑1 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGt > 1:226020/1‑69 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGt < 2:226020/69‑1 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGc < 2:406350/85‑1 (MQ=255) tataAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGc > 1:406350/1‑85 (MQ=255) | AAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAAT > NC_000913/2286002‑2286271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |