Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,322,150 A→C 5.3% L95L (CTA→CTC atoC → fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,322,1500AC 5.3% 276.1 / 6.5 75L95L (CTA→CTCatoCfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand):  ref base A (36/35);  new base C (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCAC  >  NC_000913/2322017‑2322297
                                                                                                                                     |                                                                                                                                                    
tggtgTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCtt                                                                                                                                       >  2:485326/1‑148 (MQ=255)
      tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc                                                                                                                                                   >  4:882075/1‑131 (MQ=255)
      tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGc                                                                                                                                                   <  3:882075/131‑1 (MQ=255)
      tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc                                                                                                                                           <  3:282964/139‑1 (MQ=255)
      tGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGc                                                                                                                                           >  4:282964/1‑139 (MQ=255)
                  gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCg                                                                                                                                     >  3:71872/1‑132 (MQ=255)
                  gCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCg                                                                                                                                     <  4:71872/132‑1 (MQ=255)
                         agagATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGAt                                                                                                              <  1:485326/148‑1 (MQ=255)
                          gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGt                                                                                                                   <  1:122067/142‑1 (MQ=255)
                          gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGt                                                                                                                   >  2:122067/1‑142 (MQ=255)
                                 aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGa                                                                                                         >  1:422085/1‑145 (MQ=255)
                                 aCGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGa                                                                                                         <  2:422085/145‑1 (MQ=255)
                                      aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg                                                                                                     <  1:181876/144‑1 (MQ=255)
                                      aTCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAg                                                                                                     >  2:181876/1‑144 (MQ=255)
                                       tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCg                                                                                                                                     >  2:801969/1‑111 (MQ=255)
                                       tCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCg                                                                                                                                     <  1:801969/111‑1 (MQ=255)
                                              aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGa                                                                                                         >  2:739549/1‑132 (MQ=255)
                                              aCTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGa                                                                                                         <  1:739549/132‑1 (MQ=255)
                                               cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGttatta                                                                                                                         >  3:828110/1‑115 (MQ=255)
                                               cTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGttatta                                                                                                                         <  4:828110/115‑1 (MQ=255)
                                                 aaaGGAGATGCGCAGCCATGAGATCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTGCGACTATGTTATTAAACCGTGTGATGTCGATGAGTTGAAGTTAATCGtt                                                                                      >  3:964028/1‑148 (MQ=255)
                                                   aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg                                                                                                                                              >  2:490153/1‑91 (MQ=255)
                                                   aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg                                                                                                                                              <  1:490153/91‑1 (MQ=255)
                                                   aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          >  1:600870/1‑142 (MQ=255)
                                                   aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          <  2:600870/142‑1 (MQ=255)
                                                   aGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGGCTCCGCAGCGTAGcgctt                                                                                                                                       >  3:432615/1‑93 (MQ=38)
                                                    ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          <  1:367494/141‑1 (MQ=255)
                                                    ggAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          >  2:367494/1‑141 (MQ=255)
                                                        aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          <  2:5671/137‑1 (MQ=255)
                                                        aTGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          >  1:5671/1‑137 (MQ=255)
                                                            gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa                                                                                            <  2:817263/131‑1 (MQ=255)
                                                            gcAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTa                                                                                            >  1:817263/1‑131 (MQ=255)
                                                                     agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGt                                                                                                                   <  3:799178/99‑1 (MQ=255)
                                                                     agaCCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGt                                                                                                                   >  4:799178/1‑99 (MQ=255)
                                                                        cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          >  1:920666/1‑121 (MQ=255)
                                                                        cccGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAAt                                                                                          <  2:920666/121‑1 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGtt                                                                                                                             <  1:576440/76‑1 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGtt                                                                                                                             >  2:576440/1‑76 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATg                                                                                                       >  4:779514/1‑98 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATg                                                                                                       <  3:779514/98‑1 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt                                                                           >  1:927249/1‑126 (MQ=255)
                                                                                  cGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt                                                                           <  2:927249/126‑1 (MQ=255)
                                                                                    ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa                                                              >  1:660128/1‑137 (MQ=255)
                                                                                    ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa                                                              <  2:634624/137‑1 (MQ=255)
                                                                                    ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa                                                              <  2:660128/137‑1 (MQ=255)
                                                                                    ttATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCa                                                              >  1:634624/1‑137 (MQ=255)
                                                                                        tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTaaa                                                                                                                       >  2:591784/1‑76 (MQ=255)
                                                                                        tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTaaa                                                                                                                       <  1:591784/76‑1 (MQ=255)
                                                                                        tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa                                                                                                <  3:787575/99‑1 (MQ=255)
                                                                                        tCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGaa                                                                                                >  4:787575/1‑99 (MQ=255)
                                                                                           gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt                                                                                               >  4:175852/1‑97 (MQ=255)
                                                                                           gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAAt                                                                                               <  3:175852/97‑1 (MQ=255)
                                                                                           gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg                                                                               >  1:1097583/1‑113 (MQ=255)
                                                                                           gatgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAgcgcg                                                                               <  2:1097583/113‑1 (MQ=255)
                                                                                             tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg                                                                                                                                              <  4:794789/49‑1 (MQ=255)
                                                                                             tgaCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCgg                                                                                                                                              >  3:794789/1‑49 (MQ=255)
                                                                                               aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCtt                                                                                                                                       <  3:225817/53‑1 (MQ=255)
                                                                                               aCGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCtt                                                                                                                                       >  4:225817/1‑53 (MQ=255)
                                                                                                   ccTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCa                                    >  2:1001795/1‑148 (MQ=255)
                                                                                                            aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc                                                                                     <  1:445250/90‑1 (MQ=255)
                                                                                                            aaGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc                                                                                     >  2:445250/1‑90 (MQ=255)
                                                                                                                   aaCCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt                                                                           >  1:564908/1‑93 (MQ=255)
                                                                                                                   aaCCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt                                                                           <  2:564908/93‑1 (MQ=255)
                                                                                                                      catagTCGAAGGCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa                                                                        <  3:901276/89‑1 (MQ=21)
                                                                                                                      catagTCGAAGGCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa                                                                        >  4:254321/5‑93 (MQ=21)
                                                                                                                      catagTCGAAGGCTCCGCAGCGTAGCGCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACa                                                                        <  3:254321/89‑1 (MQ=21)
                                                                                                                            cGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc                                                                                     >  1:322546/1‑74 (MQ=255)
                                                                                                                            cGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTc                                                                                     <  2:322546/74‑1 (MQ=255)
                                                                                                                                 cgcTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag                                                    <  3:930602/102‑1 (MQ=255)
                                                                                                                                 cgcTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAag                                                    >  4:930602/1‑102 (MQ=255)
                                                                                                                                  gcTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATggggg     <  1:1001795/148‑1 (MQ=255)
                                                                                                                                   cTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCaa                                                             >  1:661276/1‑91 (MQ=255)
                                                                                                                                   cTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCaa                                                             <  2:661276/91‑1 (MQ=255)
                                                                                                                                    tACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGa                                                                                                 >  3:625237/1‑54 (MQ=255)
                                                                                                                                    tACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGa                                                                                                 <  4:625237/54‑1 (MQ=255)
                                                                                                                                     aCGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGCGt                                                                                                    >  1:586697/1‑50 (MQ=255)
                                                                                                                                     aCGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGt                                                                                                    <  2:586697/50‑1 (MQ=255)
                                                                                                                                     aCGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTc                                                                    >  4:830247/1‑82 (MQ=255)
                                                                                                                                     aCGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTc                                                                    <  3:830247/82‑1 (MQ=255)
                                                                                                                                     aCGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGcac  >  2:1051318/1‑148 (MQ=255)
                                                                                                                                     |                                                                                                                                                    
TGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCAC  >  NC_000913/2322017‑2322297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: