Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,348,795 T→A 5.4% intergenic (+27/+27) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,348,7950TA 5.4% 267.0 / 5.0 74intergenic (+27/+27)yfaE/inaAferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base T (34/36);  new base A (2/2);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCG  >  NC_000913/2348649‑2348938
                                                                                                                                                  |                                                                                                                                               
cGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCtt                                                                                                                                                <  4:271562/148‑1 (MQ=255)
 gCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               <  4:246907/148‑1 (MQ=255)
    cacGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGc                                                                                                                                            >  2:238608/1‑148 (MQ=255)
    cacGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGc                                                                                                                                            >  1:142998/1‑148 (MQ=255)
    cacGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGc                                                                                                                                            >  3:20325/1‑148 (MQ=255)
      cGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               <  3:630582/143‑1 (MQ=255)
      cGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               >  4:630582/1‑143 (MQ=255)
        ccTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCt                                                                                                                                        >  2:62286/1‑148 (MQ=255)
          tGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTa                                                                                                                                      <  3:559883/148‑1 (MQ=255)
                cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                <  4:20325/148‑1 (MQ=255)
                cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                <  2:255392/148‑1 (MQ=255)
                cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                <  2:142998/148‑1 (MQ=255)
                 aGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                                                                                                                               <  1:62286/148‑1 (MQ=255)
                        gtcgACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               >  4:898770/4‑125 (MQ=255)
                        gTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               <  3:898770/125‑1 (MQ=255)
                             cTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               >  3:1066137/1‑120 (MQ=255)
                             cTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTa                                                                                                                                               <  4:1066137/120‑1 (MQ=255)
                               ggATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATAAGACGCATAAGCGTTGTATCTGGca                                                                                                                               >  3:973720/1‑133 (MQ=38)
                               ggATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATAAGACGCATAAGCGTTGTATCTGGca                                                                                                                               <  4:973720/134‑2 (MQ=38)
                                             ttAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                       <  4:956077/128‑1 (MQ=255)
                                             ttAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                       >  3:956077/1‑128 (MQ=255)
                                                   tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCa                                                                                             <  1:238608/148‑1 (MQ=255)
                                                                  gAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCAcc                                                                                           >  1:1097026/1‑135 (MQ=255)
                                                                  gAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCAcc                                                                                           <  2:1097026/135‑1 (MQ=255)
                                                                   aaaTTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGc                                                                             >  3:487739/1‑148 (MQ=255)
                                                                       tttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                         <  4:487739/148‑1 (MQ=255)
                                                                             cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                       >  2:964757/1‑96 (MQ=255)
                                                                             cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAtt                                                                   >  1:1042816/1‑148 (MQ=255)
                                                                             cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGACGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                       <  1:964757/96‑1 (MQ=255)
                                                                              tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTAc                                                                                                                   >  2:156949/1‑99 (MQ=255)
                                                                              tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTAc                                                                                                                   <  1:156949/99‑1 (MQ=255)
                                                                              tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg                                                                  >  1:533248/1‑148 (MQ=255)
                                                                                ttgttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                       >  3:929579/1‑125 (MQ=255)
                                                                                ttgttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                       <  4:929579/125‑1 (MQ=255)
                                                                                  gttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTc                                                              <  2:1042816/148‑1 (MQ=255)
                                                                                                     tatTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                        >  1:332729/1‑119 (MQ=255)
                                                                                                     tatTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                        <  2:332729/119‑1 (MQ=255)
                                                                                                            aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTgg                                                                                                                                 <  4:580114/55‑1 (MQ=255)
                                                                                                            aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTgg                                                                                                                                 >  3:580114/1‑55 (MQ=255)
                                                                                                            aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTc                                                                                              >  3:227200/1‑90 (MQ=255)
                                                                                                            aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTc                                                                                              <  4:227200/90‑1 (MQ=255)
                                                                                                              cGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTccc                                                                                    >  4:170808/1‑98 (MQ=255)
                                                                                                              cGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTccc                                                                                    <  3:170808/98‑1 (MQ=255)
                                                                                                                        aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCg                                                                              >  4:138312/1‑94 (MQ=255)
                                                                                                                        aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCg                                                                              <  3:138312/94‑1 (MQ=255)
                                                                                                                        aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTcc                                                             >  3:231236/1‑111 (MQ=255)
                                                                                                                        aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTcc                                                             <  4:231236/111‑1 (MQ=255)
                                                                                                                          tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                         >  3:582553/1‑129 (MQ=255)
                                                                                                                          tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                         <  2:977481/129‑1 (MQ=255)
                                                                                                                          tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                         >  1:977481/1‑129 (MQ=255)
                                                                                                                          tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                         <  4:582553/129‑1 (MQ=255)
                                                                                                                          tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCt                      <  2:533248/148‑1 (MQ=255)
                                                                                                                                tcgTGCCAGATACAATGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTc                                                                                      <  2:807755/76‑1 (MQ=255)
                                                                                                                                tAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTc                                                                                      >  1:807755/1‑78 (MQ=255)
                                                                                                                                     ccAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGa                                                                     <  1:95518/90‑1 (MQ=255)
                                                                                                                                     ccAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGa                                                                     >  2:95518/1‑90 (MQ=255)
                                                                                                                                      cAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                         >  2:1108076/1‑85 (MQ=255)
                                                                                                                                      cAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                         <  1:1108076/85‑1 (MQ=255)
                                                                                                                                        gataagaCGCATAAGCGTTGTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTa                            <  1:779013/122‑1 (MQ=255)
                                                                                                                                        gataagaCGCATAAGCGTTGTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTa                            >  2:779013/7‑128 (MQ=255)
                                                                                                                                        gATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                >  3:861179/1‑124 (MQ=255)
                                                                                                                                        gATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                <  4:861179/124‑1 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCg                                                                                                             <  1:1105334/41‑1 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCg                                                                                                             >  2:1105334/1‑41 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAt                                                                    >  2:351296/1‑82 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAt                                                                    <  1:351296/82‑1 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                <  1:323292/118‑1 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                >  2:323292/1‑118 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATc                 <  3:844160/133‑1 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAgcggcg  >  1:137836/1‑148 (MQ=255)
                                                                                                                                              aCGCTTATGCGTCTGATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATc                 >  4:844160/1‑133 (MQ=255)
                                                                                                                                               cGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATcgcg              <  2:98438/135‑1 (MQ=255)
                                                                                                                                               cGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATcgcg              >  1:98438/1‑135 (MQ=255)
                                                                                                                                                 cTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                       <  3:982703/60‑1 (MQ=255)
                                                                                                                                                 cTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                       >  4:982703/1‑60 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGCACACGCCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCG  >  NC_000913/2348649‑2348938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: