Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,348,803 C→T 6.1% intergenic (+35/+19) yfaE → / ← inaA ferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,348,8030CT 6.1% 216.2 / 3.0 65intergenic (+35/+19)yfaE/inaAferredoxin involved with ribonucleotide reductase diferric‑tyrosyl radical (Y*) cofactor maintenance/acid‑inducible Kdo/WaaP family putative kinase
Reads supporting (aligned to +/- strand):  ref base C (28/33);  new base T (2/2);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCGACGACTTT  >  NC_000913/2348657‑2348946
                                                                                                                                                  |                                                                                                                                               
ccTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCt                                                                                                                                                >  2:62286/1‑148 (MQ=255)
  tGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTa                                                                                                                                              <  3:559883/148‑1 (MQ=255)
        cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                        <  4:20325/148‑1 (MQ=255)
        cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                        <  2:255392/148‑1 (MQ=255)
        cAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGc                                                                                                                                        <  2:142998/148‑1 (MQ=255)
         aGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGcc                                                                                                                                       <  1:62286/148‑1 (MQ=255)
                       ggATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATAAGACGCATAAGCGTTGTATCTGGca                                                                                                                                       <  4:973720/134‑2 (MQ=38)
                       ggATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATAAGACGCATAAGCGTTGTATCTGGca                                                                                                                                       >  3:973720/1‑133 (MQ=38)
                                     ttAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                               <  4:956077/128‑1 (MQ=255)
                                     ttAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                               >  3:956077/1‑128 (MQ=255)
                                           tttATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCa                                                                                                     <  1:238608/148‑1 (MQ=255)
                                                          gAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCAcc                                                                                                   >  1:1097026/1‑135 (MQ=255)
                                                          gAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCAcc                                                                                                   <  2:1097026/135‑1 (MQ=255)
                                                           aaaTTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGc                                                                                     >  3:487739/1‑148 (MQ=255)
                                                               tttGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                                 <  4:487739/148‑1 (MQ=255)
                                                                     cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                               >  2:964757/1‑96 (MQ=255)
                                                                     cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAtt                                                                           >  1:1042816/1‑148 (MQ=255)
                                                                     cTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGACGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGa                                                                                                                               <  1:964757/96‑1 (MQ=255)
                                                                      tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTAc                                                                                                                           <  1:156949/99‑1 (MQ=255)
                                                                      tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTAc                                                                                                                           >  2:156949/1‑99 (MQ=255)
                                                                      tgttgtTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTg                                                                          >  1:533248/1‑148 (MQ=255)
                                                                        ttgttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                               >  3:929579/1‑125 (MQ=255)
                                                                        ttgttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                               <  4:929579/125‑1 (MQ=255)
                                                                          gttgCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTc                                                                      <  2:1042816/148‑1 (MQ=255)
                                                                                             tatTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                                >  1:332729/1‑119 (MQ=255)
                                                                                             tatTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTgg                                                                                <  2:332729/119‑1 (MQ=255)
                                                                                                    aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTgg                                                                                                                                         <  4:580114/55‑1 (MQ=255)
                                                                                                    aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTgg                                                                                                                                         >  3:580114/1‑55 (MQ=255)
                                                                                                    aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTc                                                                                                      <  4:227200/90‑1 (MQ=255)
                                                                                                    aTCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTc                                                                                                      >  3:227200/1‑90 (MQ=255)
                                                                                                      cGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTccc                                                                                            <  3:170808/98‑1 (MQ=255)
                                                                                                      cGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTccc                                                                                            >  4:170808/1‑98 (MQ=255)
                                                                                                                aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCg                                                                                      <  3:138312/94‑1 (MQ=255)
                                                                                                                aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCg                                                                                      >  4:138312/1‑94 (MQ=255)
                                                                                                                aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTcc                                                                     <  4:231236/111‑1 (MQ=255)
                                                                                                                aTTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTcc                                                                     >  3:231236/1‑111 (MQ=255)
                                                                                                                  tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                                 >  1:977481/1‑129 (MQ=255)
                                                                                                                  tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                                 <  2:977481/129‑1 (MQ=255)
                                                                                                                  tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                                 <  4:582553/129‑1 (MQ=255)
                                                                                                                  tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCg                                                 >  3:582553/1‑129 (MQ=255)
                                                                                                                  tGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCt                              <  2:533248/148‑1 (MQ=255)
                                                                                                                        tcgTGCCAGATACAATGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTc                                                                                              <  2:807755/76‑1 (MQ=255)
                                                                                                                        tAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTc                                                                                              >  1:807755/1‑78 (MQ=255)
                                                                                                                             ccAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGa                                                                             >  2:95518/1‑90 (MQ=255)
                                                                                                                             ccAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGa                                                                             <  1:95518/90‑1 (MQ=255)
                                                                                                                              cAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                                 >  2:1108076/1‑85 (MQ=255)
                                                                                                                              cAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTg                                                                                 <  1:1108076/85‑1 (MQ=255)
                                                                                                                                gataagaCGCATAAGCGTTGTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTa                                    <  1:779013/122‑1 (MQ=255)
                                                                                                                                gataagaCGCATAAGCGTTGTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTa                                    >  2:779013/7‑128 (MQ=255)
                                                                                                                                gATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                        >  3:861179/1‑124 (MQ=255)
                                                                                                                                gATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                        <  4:861179/124‑1 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCg                                                                                                                     <  1:1105334/41‑1 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCg                                                                                                                     >  2:1105334/1‑41 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAt                                                                            <  1:351296/82‑1 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGAt                                                                            >  2:351296/1‑82 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                        >  2:323292/1‑118 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATg                                        <  1:323292/118‑1 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATc                         <  3:844160/133‑1 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAgcggcg          >  1:137836/1‑148 (MQ=255)
                                                                                                                                      aCGCTTATGCGTCTGATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATc                         >  4:844160/1‑133 (MQ=255)
                                                                                                                                       cGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATcgcg                      >  1:98438/1‑135 (MQ=255)
                                                                                                                                       cGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATcgcg                      <  2:98438/135‑1 (MQ=255)
                                                                                                                                         cTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                               <  3:982703/60‑1 (MQ=255)
                                                                                                                                         cTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCt                                                                                               >  4:982703/1‑60 (MQ=255)
                                                                                                                                              gCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCGACGACttt  <  2:137836/148‑1 (MQ=255)
                                                                                                                                                  cTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTg                                                    <  4:395433/94‑1 (MQ=255)
                                                                                                                                                  cTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTg                                                    >  3:395433/1‑94 (MQ=255)
                                                                                                                                                  cTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGa                                       >  3:991720/1‑107 (MQ=255)
                                                                                                                                                  cTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGa                                       <  4:991720/107‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CCTGGTTGCAGGTCAAGTTGACTGGATTGCCGAACCGTTAGCCTTTATTCAGCCGGGGGAAATTTTGCCCTGTTGTTGCCGGGCAAAAGGCGATATTGAAATCGAGATGTGAATTGGTTGTAGTGCCAGATACAACGCTTATGCGTCTTATCTGGCCTACAACGATTACATGGCGTAGTAATACGCTTTCACCTGCTCCCAGTCCGCTTTGGGGATTGGCTCCAGATATTTTTCCAGCTGGCGGAAGTCATGATTAATCGCTTTATCGCGACGCAAGCGGCGACGACTTT  >  NC_000913/2348657‑2348946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: