Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,684 T→A 5.4% Q291L (CAG→CTG)  nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,6840TA 5.4% 418.9 / 10.2 112Q291L (CAG→CTG) nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base T (51/55);  new base A (3/3);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 112 total aligned reads displayed.

GCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCT  >  NC_000913/2400538‑2400827
                                                                                                                                                  |                                                                                                                                               
gCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCGAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTg                                                                                                                                                <  3:673278/148‑1 (MQ=255)
 cACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGc                                                                                                                                               <  3:725843/148‑1 (MQ=255)
    cAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                            <  2:680183/148‑1 (MQ=255)
     aGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                                                                                                                              >  2:930576/1‑145 (MQ=255)
     aGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGcc                                                                                                                                              <  1:930576/145‑1 (MQ=255)
      gCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGc                                                                                                                                          <  3:1083336/148‑1 (MQ=255)
      gCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGc                                                                                                                                          <  3:451636/148‑1 (MQ=255)
          cacCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTa                                                                                                                                      <  3:485265/148‑1 (MQ=255)
              aTGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAAtt                                                                                                                                  >  1:465512/1‑148 (MQ=255)
                gTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                <  2:7393/148‑1 (MQ=255)
                gTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                <  2:465512/148‑1 (MQ=255)
                gTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcg                                                                                                                                         <  3:930313/139‑2 (MQ=255)
                gTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCAGCCAGGcg                                                                                                                                         >  4:930313/1‑138 (MQ=255)
                    aTCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGAc                                                                                                                            >  2:309445/1‑148 (MQ=255)
                      ctcATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCa                                                                                                                          >  4:463182/1‑148 (MQ=255)
                              cAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                            >  4:626319/1‑122 (MQ=255)
                              cAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAg                                                                                                                                            <  3:626319/122‑1 (MQ=255)
                                aCCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCa                                                                                                                       <  4:1001740/141‑1 (MQ=255)
                                aCCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCa                                                                                                                       >  3:1001740/1‑141 (MQ=255)
                                     cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                <  4:23684/127‑1 (MQ=255)
                                     cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                >  3:23684/1‑127 (MQ=255)
                                     cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                >  3:299115/1‑127 (MQ=255)
                                     cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                <  2:185451/127‑1 (MQ=255)
                                     cATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                >  1:185451/1‑127 (MQ=255)
                                            aGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAAt                                                                                                    <  1:309445/148‑1 (MQ=255)
                                                 cGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCg                                                                                               <  3:463182/148‑1 (MQ=255)
                                                       cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAgg                                                                                                                                           <  1:496486/98‑1 (MQ=255)
                                                       cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                <  1:494964/109‑1 (MQ=255)
                                                       cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTc                                                                                                                                >  2:494964/1‑109 (MQ=255)
                                                       cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGa                                                                                                                             <  1:707898/112‑1 (MQ=255)
                                                       cAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGa                                                                                                                             >  2:707898/1‑112 (MQ=255)
                                                        aGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGa                                                                                           <  4:1135395/145‑1 (MQ=255)
                                                                gCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCg                                                                                >  1:79885/1‑148 (MQ=255)
                                                                 cccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAg                                                                                                                              <  4:759372/101‑1 (MQ=255)
                                                                 cccGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAg                                                                                                                              >  3:759372/1‑101 (MQ=255)
                                                                            gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAgg                                                                                                                                           <  4:326615/77‑1 (MQ=255)
                                                                            gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAgg                                                                                                                                           >  3:326615/1‑77 (MQ=255)
                                                                            gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                               <  3:4306/89‑1 (MQ=255)
                                                                            gATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCa                                                                                                                               >  4:4306/1‑89 (MQ=255)
                                                                              tACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt                                                                                                                         <  4:436109/93‑1 (MQ=255)
                                                                              tACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt                                                                                                                         >  3:436109/1‑93 (MQ=255)
                                                                                  ttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCg                                                                                               >  4:772522/1‑115 (MQ=255)
                                                                                  ttCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCg                                                                                               <  3:772522/115‑1 (MQ=255)
                                                                                  ttCGAATTCCACCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTg                                                                                                                                                >  3:759584/1‑66 (MQ=255)
                                                                                  ttCGAATTCCACCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTg                                                                                                                                                <  4:759584/66‑1 (MQ=255)
                                                                                   tCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGtt                                                             >  2:590050/1‑148 (MQ=255)
                                                                                   tCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGtt                                                             <  2:79885/148‑1 (MQ=255)
                                                                                            aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtgg                                                                            >  1:80749/1‑124 (MQ=255)
                                                                                            aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtgg                                                                            <  2:80749/124‑1 (MQ=255)
                                                                                            aTCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTc                                                    >  2:180648/1‑148 (MQ=255)
                                                                                                 cAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                             >  2:260901/1‑118 (MQ=255)
                                                                                                 cAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCggtg                                                                             <  1:260901/118‑1 (MQ=255)
                                                                                                    aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTcgcg                                                                                    >  3:1087192/1‑108 (MQ=255)
                                                                                                    aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTcgcg                                                                                    <  4:1087192/108‑1 (MQ=255)
                                                                                                    aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                          >  1:746759/1‑134 (MQ=255)
                                                                                                    aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                          <  2:746759/134‑1 (MQ=255)
                                                                                                    aTCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAttt                                            >  3:707283/1‑148 (MQ=255)
                                                                                                     tCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtttt                                           >  2:256137/1‑148 (MQ=255)
                                                                                                      cAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATacc                                                                                                               >  1:223595/1‑79 (MQ=255)
                                                                                                      cAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAttttt                                          <  4:701995/148‑1 (MQ=255)
                                                                                                       aaGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTc                                         <  1:256137/148‑1 (MQ=255)
                                                                                                        aGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                          <  4:1033709/130‑1 (MQ=255)
                                                                                                        aGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTccc                                                          >  3:1033709/1‑130 (MQ=255)
                                                                                                         ggTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCAc                                       <  4:707283/148‑1 (MQ=255)
                                                                                                             cgcTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCgg                                                <  1:780805/135‑1 (MQ=255)
                                                                                                                      cAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGaa                                                                     <  3:192840/105‑1 (MQ=255)
                                                                                                                      cAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGaa                                                                     >  4:192840/1‑105 (MQ=255)
                                                                                                                      cAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGCACGGCAGTTCCCACCGCCCCGGATTTTTCACCCGACCGGCGAAg                          >  3:55622/1‑148 (MQ=255)
                                                                                                                        ggAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGcc                        <  1:180648/148‑1 (MQ=255)
                                                                                                                          aaGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGTACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCa                      >  4:98634/1‑148 (MQ=255)
                                                                                                                          aaGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGa                                   >  3:180737/1‑135 (MQ=255)
                                                                                                                          aaGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGa                                   <  4:180737/135‑1 (MQ=255)
                                                                                                                          aaGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCa                      >  1:478203/1‑148 (MQ=255)
                                                                                                                            gtcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATc                    <  1:590050/148‑1 (MQ=255)
                                                                                                                             tcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCgg                               <  4:723327/136‑1 (MQ=255)
                                                                                                                             tcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCgg                               >  3:723327/1‑136 (MQ=255)
                                                                                                                             tcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                          <  3:205530/141‑1 (MQ=255)
                                                                                                                             tcagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAg                          >  4:205530/1‑141 (MQ=255)
                                                                                                                              cagtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTc                                         >  3:654419/1‑125 (MQ=255)
                                                                                                                                gtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACg                                                                                                                     <  1:361248/47‑1 (MQ=255)
                                                                                                                                gtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACg                                                                                                                     >  2:361248/1‑47 (MQ=255)
                                                                                                                                gtcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCt                <  2:478203/148‑1 (MQ=255)
                                                                                                                                 tcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTc                                         >  1:195783/1‑122 (MQ=255)
                                                                                                                                 tcCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTc                                         <  2:195783/122‑1 (MQ=255)
                                                                                                                                  ccccGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtttt                                           <  2:462054/119‑1 (MQ=255)
                                                                                                                                     cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt                                                                                                                         <  2:739094/38‑1 (MQ=255)
                                                                                                                                     cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCAt                                                                                                                         >  1:739094/1‑38 (MQ=255)
                                                                                                                                     cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                  >  1:905471/1‑109 (MQ=255)
                                                                                                                                     cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                  <  2:905471/109‑1 (MQ=255)
                                                                                                                                     cgcgCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTg           >  3:715886/1‑148 (MQ=255)
                                                                                                                                       taaagccTGGCAGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                  <  4:593282/103‑1 (MQ=255)
                                                                                                                                       taaagccTGGCAGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                  >  3:994708/5‑107 (MQ=255)
                                                                                                                                       taaagccTGGCAGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTccc                                                  >  3:593282/5‑107 (MQ=255)
                                                                                                                                       cgccgcCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCgg                                                >  4:460697/1‑109 (MQ=255)
                                                                                                                                       cgccgcCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtt                                             <  2:224328/112‑1 (MQ=255)
                                                                                                                                       cgccgcCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGAtt                                             >  1:224328/1‑112 (MQ=255)
                                                                                                                                           gccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGcc                        >  4:516860/1‑129 (MQ=255)
                                                                                                                                           gccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGcc                        <  3:516860/129‑1 (MQ=255)
                                                                                                                                              tGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCt  >  2:647128/1‑148 (MQ=255)
                                                                                                                                               ggCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACg                                                                   >  2:773700/1‑82 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCAGATCAAGGTGCGCTTCGGTCAGGAAGTCAGTCCCCGCGCCGCCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCT  >  NC_000913/2400538‑2400827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: