| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,411,963 | T→C | 6.3% | I43V (ATT→GTT) | yfbT ← | sugar phosphatas |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,411,963 | 0 | T | C | 6.3% | 213.0 / 3.9 | 63 | I43V (ATT→GTT) | yfbT | sugar phosphatas |
| Reads supporting (aligned to +/- strand): ref base T (30/29); new base C (2/2); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
CGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGGCCTCCTCAGGCATTG > NC_000913/2411832‑2412109 | cGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCa < 4:263322/143‑1 (MQ=255)cGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCa > 3:263322/1‑143 (MQ=255) ccTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAg < 4:614991/121‑1 (MQ=255) ccTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAg > 3:614991/1‑121 (MQ=255) cTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAACGAAAGCCAGCACCTCTTCCGGCGCTa < 1:171793/141‑1 (MQ=255) cTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAACGAAAGCCAGCACCTCTTCCGGCGCTa > 2:171793/1‑141 (MQ=255) ttCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaatg < 3:1074042/115‑1 (MQ=255) ttCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGtgaatg > 4:1074042/1‑115 (MQ=255) tCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc < 2:798179/119‑1 (MQ=255) tCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc > 1:798179/1‑119 (MQ=255) tCGTTGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc < 2:287983/104‑1 (MQ=255) tCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGc > 1:287983/1‑104 (MQ=255) tCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTc > 2:122825/1‑136 (MQ=255) tCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTc < 1:122825/136‑1 (MQ=255) tCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGc > 3:715318/1‑148 (MQ=255) gATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTc < 4:715318/148‑1 (MQ=255) ccAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACctc < 1:557117/103‑1 (MQ=255) ccAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACctc > 2:557117/1‑103 (MQ=255) cgTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCt > 1:493812/1‑120 (MQ=255) cgTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCt < 2:493812/120‑1 (MQ=255) tAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAccc > 2:57735/1‑109 (MQ=255) tAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAccc < 1:57735/109‑1 (MQ=255) tAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGtt > 1:722033/1‑141 (MQ=255) tAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGtt < 2:722033/141‑1 (MQ=255) aaaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTc < 3:775709/96‑1 (MQ=255) aaaCTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTc > 4:775709/1‑96 (MQ=255) cggcggCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTg < 3:4546/113‑1 (MQ=255) cggcggCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTg > 4:4546/1‑113 (MQ=255) gcAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAAt > 1:1022724/1‑148 (MQ=255) aGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGc > 2:1064316/1‑148 (MQ=255) cGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTaa < 2:22731/82‑1 (MQ=255) cGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTaa > 1:22731/1‑82 (MQ=255) cGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACg > 4:356997/1‑91 (MQ=255) cGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACg < 3:356997/91‑1 (MQ=255) tttGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACctct < 2:146139/67‑1 (MQ=255) tttGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACctct > 1:146139/1‑67 (MQ=255) gCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGa < 2:1022724/148‑1 (MQ=255) ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATc > 2:613008/1‑148 (MQ=255) gCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCaa < 1:613008/148‑1 (MQ=255) gCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCaa < 1:1064316/148‑1 (MQ=255) gCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAACGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGc < 4:138656/94‑1 (MQ=255) gCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAACGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGc > 3:138656/1‑94 (MQ=255) ccATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa > 4:259476/1‑108 (MQ=255) ccATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa < 3:259476/108‑1 (MQ=255) cattttaTGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGtt < 4:816023/127‑1 (MQ=255) cattttaTGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGtt > 3:816023/7‑133 (MQ=255) tAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa < 3:392352/105‑1 (MQ=255) tAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa > 4:392352/1‑105 (MQ=255) agagGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCgcaggca > 3:954575/1‑101 (MQ=255) agagGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCgcaggca < 4:954575/101‑1 (MQ=255) aTCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCa > 1:413746/1‑117 (MQ=255) aTCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCa < 2:413746/117‑1 (MQ=255) aTCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCAcgc > 3:125715/1‑148 (MQ=255) cGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGa > 3:1062422/1‑96 (MQ=255) cGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGa < 4:1062422/96‑1 (MQ=255) cGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCaa > 2:218008/1‑122 (MQ=255) cGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCaa < 1:218008/122‑1 (MQ=255) ccTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCtt > 2:297061/1‑132 (MQ=255) ccTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCtt < 1:297061/132‑1 (MQ=255) tttACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAg < 4:1141966/99‑1 (MQ=255) tttACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAg > 3:1141966/1‑99 (MQ=255) cGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGaaa < 3:976695/118‑1 (MQ=255) cGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGaaa > 4:976695/1‑118 (MQ=255) gTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATcca < 2:1016078/89‑1 (MQ=255) gTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATcca > 1:1016078/1‑89 (MQ=255) atgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGGCCTCCTCAGGCATTg > 2:803565/1‑148 (MQ=255) tgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCAc < 1:214592/122‑1 (MQ=255) tgaaAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCAc > 2:214592/1‑122 (MQ=255) | CGGAAGCGCGGTAATACCTTCGGTTTCCGTGGCCTCGATGTGCTCCAGACGCGTAAACTCGGCGGCAATATCAGCCTCGGATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCAAACAGAAAACCTTTGCACCGCACGCGGGCCTCCTCAGGCATTG > NC_000913/2411832‑2412109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |