Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,640 G→T 5.4% intergenic (‑46/+37) yfbU ← / ← yfbV UPF0304 family protein/UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,6400GT 5.4% 317.3 / 5.3 89intergenic (‑46/+37)yfbU/yfbVUPF0304 family protein/UPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (41/43);  new base T (2/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGC  >  NC_000913/2412496‑2412755
                                                                                                                                                |                                                                                                                   
ttGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGa                                                                                                                    <  3:149025/146‑1 (MQ=255)
ttGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGa                                                                                                                    >  4:149025/1‑146 (MQ=255)
              aaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttctt                                                                                                        <  3:380889/144‑1 (MQ=255)
              aaCGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttctt                                                                                                        >  4:380889/1‑144 (MQ=255)
                  ttCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGc                                                                                                >  2:462660/1‑148 (MQ=255)
                   tCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTa                                                                                                                 >  4:851208/1‑130 (MQ=255)
                   tCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTa                                                                                                                 <  3:851208/130‑1 (MQ=255)
                       cATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttctt                                                                                                        <  4:607498/135‑1 (MQ=255)
                       cATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttctt                                                                                                        >  3:607498/1‑135 (MQ=255)
                       cATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttt                                                                                                       <  2:586553/136‑1 (MQ=255)
                       cATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttt                                                                                                       >  1:586553/1‑136 (MQ=255)
                                 aTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTg                                                                                                                     <  1:208483/112‑1 (MQ=255)
                                 aTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTg                                                                                                                     >  2:208483/1‑112 (MQ=255)
                                       cATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATg                                                                                                               >  3:867086/1‑112 (MQ=255)
                                       cATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATg                                                                                                               <  4:867086/112‑1 (MQ=255)
                                             catcatCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACaaa                                                                          <  1:123974/143‑1 (MQ=255)
                                             catcatCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACaaa                                                                          >  2:123974/1‑143 (MQ=255)
                                             catcatCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAatcatc                                                                     >  1:780380/1‑148 (MQ=255)
                                               tcatcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCctt                                                                                                           <  4:902086/108‑1 (MQ=255)
                                               tcatcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCctt                                                                                                           >  3:902086/1‑108 (MQ=255)
                                                catcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttttt                                                                                                     >  2:82687/1‑112 (MQ=38)
                                                catcTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttttt                                                                                                     <  1:82687/113‑2 (MQ=39)
                                                     tGTACTTATTAAACAAAATAAGACGTTGAGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                      <  3:1077282/107‑1 (MQ=255)
                                                     tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                      >  4:1077282/1‑107 (MQ=255)
                                                     tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAg                                                                                        <  4:529815/121‑1 (MQ=255)
                                                     tGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAg                                                                                        >  3:529815/1‑121 (MQ=255)
                                                      gTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                            <  3:340199/148‑1 (MQ=255)
                                                             ttAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat                                                                                                    >  4:326880/1‑101 (MQ=255)
                                                             ttAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat                                                                                                    <  3:326880/101‑1 (MQ=255)
                                                                 aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttct                                                                                                         <  1:804413/92‑1 (MQ=255)
                                                                 aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCcttct                                                                                                         >  2:804413/1‑92 (MQ=255)
                                                                 aCAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCATACTCAGCCTTCttt                                                                                                       <  1:428557/94‑1 (MQ=38)
                                                                  cAAAATAAGATGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACaa                                                                           <  1:850524/121‑1 (MQ=255)
                                                                  cAAAATAAGATGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACaa                                                                           >  2:850524/1‑121 (MQ=255)
                                                                  cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTAt                                                                                                                >  1:834420/1‑84 (MQ=255)
                                                                  cAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTAt                                                                                                                <  2:834420/84‑1 (MQ=255)
                                                                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTTACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                      <  3:465975/92‑1 (MQ=255)
                                                                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCtttt                                                                                                      >  4:465975/1‑92 (MQ=255)
                                                                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCa                                                                                         >  2:367421/1‑105 (MQ=255)
                                                                    aaaTAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCa                                                                                         <  1:367421/105‑1 (MQ=255)
                                                                         aGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAgg                                                                                       >  2:825932/1‑102 (MQ=255)
                                                                         aGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAgg                                                                                       <  1:825932/102‑1 (MQ=255)
                                                                          gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCa                                                                                         <  3:138067/99‑1 (MQ=255)
                                                                          gACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCa                                                                                         >  4:138067/1‑99 (MQ=255)
                                                                              ttGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGa                                                                                                                    <  2:714743/68‑1 (MQ=255)
                                                                              ttGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGa                                                                                                                    >  1:714743/1‑68 (MQ=255)
                                                                                      tGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCg                            <  1:462660/148‑1 (MQ=255)
                                                                                      tGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCg                            <  2:780380/148‑1 (MQ=255)
                                                                                         tCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                   >  2:567535/1‑106 (MQ=255)
                                                                                         tCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                   <  1:567535/106‑1 (MQ=255)
                                                                                            tttCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat                                                                                                    >  1:1008046/1‑70 (MQ=255)
                                                                                            tttCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTtat                                                                                                    <  2:1008046/70‑1 (MQ=255)
                                                                                            tttCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAATCGTGTCAg                      >  2:917237/1‑148 (MQ=255)
                                                                                            tttCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAg                      >  3:724922/1‑148 (MQ=255)
                                                                                                  ttgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                          >  2:769809/1‑106 (MQ=255)
                                                                                                  ttgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGttt                                                          <  1:769809/106‑1 (MQ=255)
                                                                                                   tgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGt                                                                                    <  4:444010/79‑1 (MQ=255)
                                                                                                   tgttgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGt                                                                                    >  3:444010/1‑79 (MQ=255)
                                                                                                      tgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCttt                                                                                                       >  4:836085/1‑57 (MQ=255)
                                                                                                      tgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCttt                                                                                                       <  3:836085/57‑1 (MQ=255)
                                                                                                      tgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGaa                                          >  3:273691/1‑118 (MQ=255)
                                                                                                      tgACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGaa                                          <  4:273691/118‑1 (MQ=255)
                                                                                                           ccTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtttt                                                         >  2:624586/1‑98 (MQ=255)
                                                                                                           ccTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGtttt                                                         <  1:624586/98‑1 (MQ=255)
                                                                                                               tATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAgg   <  4:724922/148‑1 (MQ=255)
                                                                                                                aTCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTACTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc  <  1:917237/148‑1 (MQ=255)
                                                                                                                 tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCa                                                    <  2:192783/97‑1 (MQ=255)
                                                                                                                 tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAg                              <  1:452996/119‑1 (MQ=255)
                                                                                                                 tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAg                              >  2:452996/1‑119 (MQ=255)
                                                                                                                 tCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTACCCa                                                    >  1:192783/1‑97 (MQ=255)
                                                                                                                     tctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                            <  1:946101/85‑1 (MQ=255)
                                                                                                                     tctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGt                                                            >  2:946101/1‑85 (MQ=255)
                                                                                                                     tctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgt                                               <  2:224637/98‑1 (MQ=255)
                                                                                                                     tctACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgt                                               >  1:224637/1‑98 (MQ=255)
                                                                                                                      ctactaCGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAg                                                             <  1:1063800/83‑1 (MQ=255)
                                                                                                                      ctactaCGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAg                                                             >  2:1063800/1‑83 (MQ=255)
                                                                                                                             ggTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTa                                                        <  3:819823/81‑1 (MQ=255)
                                                                                                                             agTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTCTTa                                                        >  4:819823/2‑81 (MQ=255)
                                                                                                                               tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                   <  3:461545/68‑1 (MQ=255)
                                                                                                                               tGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCaa                                                                   >  4:461545/1‑68 (MQ=255)
                                                                                                                                  aaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtgt                         >  2:631162/1‑107 (MQ=255)
                                                                                                                                  aaaaaaaGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCgtgt                         <  1:631162/107‑1 (MQ=255)
                                                                                                                                        agaagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                              >  2:423682/1‑80 (MQ=255)
                                                                                                                                        agaagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgttgtt                                              <  1:423682/80‑1 (MQ=255)
                                                                                                                                         gaagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc              >  3:366255/1‑111 (MQ=255)
                                                                                                                                         gaagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGcc              <  4:366255/111‑1 (MQ=255)
                                                                                                                                          aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgt                                                  >  2:353014/1‑74 (MQ=255)
                                                                                                                                          aagGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAgt                                                  <  1:353014/74‑1 (MQ=255)
                                                                                                                                              cggAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGa                                           >  2:425756/3‑77 (MQ=255)
                                                                                                                                              cTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGa                                           <  1:425756/77‑1 (MQ=255)
                                                                                                                                               tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc  <  3:988467/117‑1 (MQ=255)
                                                                                                                                               tGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGc  >  4:988467/1‑117 (MQ=255)
                                                                                                                                                gAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                               >  2:1120522/1‑39 (MQ=255)
                                                                                                                                                gAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAAtt                                                                               <  1:1120522/39‑1 (MQ=255)
                                                                                                                                                |                                                                                                                   
TTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTATATGCGTAATCAGGGGTCAATTACAAATCATCAAGGAAAGTTTTATCCAGTTGTTTGAAGGCGCGCTTAAGCGTGTCAGCTAATGCCTGGTAATCAGGC  >  NC_000913/2412496‑2412755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: