Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,491,264 T→A 6.2% E229D (GAA→GAT) ‡ oxc ← oxalyl CoA decarboxylase, ThDP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,491,2640TA 6.2% 295.0 / 7.7 80E229D (GAA→GAT) ‡oxcoxalyl CoA decarboxylase, ThDP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (36/39);  new base A (3/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTA  >  NC_000913/2491118‑2491396
                                                                                                                                                  |                                                                                                                                     
ccAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGtt                                                                                                                                      >  4:366675/1‑148 (MQ=255)
    caaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatc                                                                                                                                  >  3:953932/1‑148 (MQ=255)
    caaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                    >  2:48421/1‑146 (MQ=255)
    caaGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtca                                                                                                                                    <  1:48421/146‑1 (MQ=255)
       gCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGc                                                                                                                               <  4:953932/148‑1 (MQ=255)
        cATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCt                                                                                                                              <  2:546313/148‑1 (MQ=255)
        cATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCt                                                                                                                              >  3:880991/1‑148 (MQ=255)
          tGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑g                                                                                                                           <  3:366675/148‑1 (MQ=255)
            aCAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTt‑gtg                                                                                                                         <  3:8310/148‑1 (MQ=255)
               aCGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAAt                                                                                                                      <  4:880991/148‑1 (MQ=255)
                   cAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATAcgc                                                                                                                  >  3:340299/1‑148 (MQ=255)
                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCttcg                                                                                                                           <  4:532365/137‑3 (MQ=255)
                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCttcg                                                                                                                           >  3:532365/1‑135 (MQ=255)
                     gCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCAC‑AAGCTGATGAACAGCttcg                                                                                                                           >  1:301477/1‑135 (MQ=255)
                           gCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCtt                                                                                                          <  4:340299/148‑1 (MQ=255)
                            ccAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCttt                                                                                                         <  2:950391/148‑1 (MQ=255)
                              aGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTGAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGt                                                                                                                                       >  3:777782/1‑117 (MQ=255)
                              aGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGt                                                                                                                                       <  4:777782/117‑1 (MQ=255)
                                                          aaaGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCttt                                                                           >  3:268673/1‑148 (MQ=255)
                                                                 aTGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaa                                                                    <  4:268673/148‑1 (MQ=255)
                                                                    cGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                             >  1:607890/1‑120 (MQ=255)
                                                                    cGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaat                                                                                             <  2:607890/120‑1 (MQ=255)
                                                                       aTCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          <  1:782750/120‑1 (MQ=255)
                                                                       aTCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          >  2:782750/1‑120 (MQ=255)
                                                                        tCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCaa                                                                                                    <  3:970371/109‑1 (MQ=255)
                                                                        tCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCaa                                                                                                    >  4:970371/1‑109 (MQ=255)
                                                                           tCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGt                                                                                                                                       <  1:631563/72‑1 (MQ=255)
                                                                           tCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGt                                                                                                                                       >  2:631563/1‑72 (MQ=255)
                                                                           tCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                          >  3:477278/1‑148 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatca                                                                                                                                 >  3:914099/1‑77 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatca                                                                                                                                 <  4:914099/77‑1 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatca                                                                                                                                 >  1:222159/1‑77 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtcatca                                                                                                                                 <  2:222159/77‑1 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTGATGGc                                                                                      >  1:918795/1‑119 (MQ=255)
                                                                            cAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGc                                                                                      <  2:918795/119‑1 (MQ=255)
                                                                                   cccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCGATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          <  4:1061817/108‑1 (MQ=255)
                                                                                   cccTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          >  3:1061817/1‑108 (MQ=255)
                                                                                     cTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgac                                                >  1:1095781/1‑148 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtc                                                                                                                                     <  4:636794/63‑1 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTtc                                                                                                                                     >  3:636794/1‑63 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCg                                                                                    <  1:194745/111‑1 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAg                                                                               >  3:1094512/1‑116 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAg                                                                               <  4:1094512/116‑1 (MQ=255)
                                                                                      tttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTAATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCg                                                                                    >  2:194745/1‑111 (MQ=255)
                                                                                       ttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGt                                                                                   <  4:652128/111‑1 (MQ=255)
                                                                                       ttCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGt                                                                                   >  3:652128/1‑111 (MQ=255)
                                                                                              tAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTa                                                     >  4:658363/1‑134 (MQ=255)
                                                                                              tAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTa                                                     <  3:658363/134‑1 (MQ=255)
                                                                                                  cATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                          <  2:534984/125‑1 (MQ=255)
                                                                                                  cATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTg                                                          >  1:534984/1‑125 (MQ=255)
                                                                                                        cAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAg                                                    >  1:481061/1‑125 (MQ=255)
                                                                                                        cAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAg                                                    <  2:481061/125‑1 (MQ=255)
                                                                                                           gaatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCaataat                          <  4:477278/148‑1 (MQ=255)
                                                                                                            aatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgac                                                >  1:39612/1‑125 (MQ=255)
                                                                                                            aatggaatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgac                                                <  2:39612/125‑1 (MQ=255)
                                                                                                                 aatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGATT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGGGAAATTGCGCTAGTGACTGACTTc                                         <  1:116109/127‑1 (MQ=255)
                                                                                                                 aatCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGATT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGGGAAATTGCGCTAGTGACTGACTTc                                         >  2:116109/1‑127 (MQ=255)
                                                                                                                  atCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          <  4:831844/77‑1 (MQ=255)
                                                                                                                  atCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGAtaattaa                                                                                          >  3:831844/1‑77 (MQ=255)
                                                                                                                   tCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGa                                                                                                 >  2:994506/1‑69 (MQ=255)
                                                                                                                   tCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGa                                                                                                 <  1:994506/69‑1 (MQ=255)
                                                                                                                   tCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCg                                        >  3:65026/1‑126 (MQ=255)
                                                                                                                   tCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCg                                        <  4:65026/126‑1 (MQ=255)
                                                                                                                      gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaa                                                                    <  1:882950/95‑1 (MQ=255)
                                                                                                                      gAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTaaa                                                                    >  2:882950/1‑95 (MQ=255)
                                                                                                                        gCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAAt                                                                                                                      <  3:501400/43‑1 (MQ=255)
                                                                                                                        gCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAAt                                                                                                                      >  4:501400/1‑43 (MQ=255)
                                                                                                                             ttCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCtttt                                                                          >  2:466161/1‑82 (MQ=255)
                                                                                                                             ttCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCtttt                                                                          <  1:466161/82‑1 (MQ=255)
                                                                                                                                 aTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactg                                              >  4:613105/1‑106 (MQ=255)
                                                                                                                                 aTAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtgactg                                              <  3:613105/106‑1 (MQ=255)
                                                                                                                                  tAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt             >  3:106089/1‑138 (MQ=255)
                                                                                                                                  tAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGAt             <  4:106089/138‑1 (MQ=255)
                                                                                                                                  tAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACttt   >  2:1095782/1‑148 (MQ=255)
                                                                                                                                   aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAg                                                    <  4:1090556/98‑1 (MQ=255)
                                                                                                                                   aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAg                                                    >  3:1090556/1‑98 (MQ=255)
                                                                                                                                   aaaTTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa  <  1:1095782/148‑1 (MQ=255)
                                                                                                                                       tCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtga                                                 <  1:694505/97‑1 (MQ=255)
                                                                                                                                       tCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGtga                                                 >  2:694505/1‑97 (MQ=255)
                                                                                                                                        tcacaaGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCag                                   >  3:542064/5‑110 (MQ=38)
                                                                                                                                        tcacaaGCTGATGAACAGCTTCGTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCAt                                   <  4:542064/107‑1 (MQ=38)
                                                                                                                                            aaGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCa        <  3:323894/133‑1 (MQ=255)
                                                                                                                                            aaGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCa        >  4:323894/1‑133 (MQ=255)
                                                                                                                                               cTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATGGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa  >  1:317547/1‑136 (MQ=255)
                                                                                                                                               cTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGCATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTa  <  2:317547/136‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                     
CCAACAAGCATGACAACGTCAGCATTTGCCAGGGCAAACGAACGCGCAGCTGCCGCAGAAAGTGGATGCGTATCTTCAAGGATCCCTTTCGCCATAGACATTGGCAGGAATGGAATCTGAGCACTTTCAATAAATTCACGAAGCTGTTCATCAGCTT‑GTGAATACGCCGCGCCTTTGCCAAGGATAATTAATGGCCGTTCAGCTTTTGCTAAAAGCGAAATTGCGCTAGTGACTGACTTCGGGCATGGCAATAATGCTGGCGACGGATTTTCAACTTTA  >  NC_000913/2491118‑2491396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑