Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,566,267 T→G 10.6% A206A (GCA→GCC eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,2670TG10.6% 299.3 / 29.2 94A206A (GCA→GCCeutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base T (43/41);  new base G (5/5);  total (48/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

TGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566129‑2566414
                                                                                                                                          |                                                                                                                                                   
tGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGacg                                                                                                                                            <  2:465964/148‑3 (MQ=255)
tGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGacg                                                                                                                                            >  1:465964/1‑146 (MQ=255)
tGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAg                                                                                                                                            >  1:1010116/1‑148 (MQ=255)
           aaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCcac                                                                                                                                 >  2:944571/1‑148 (MQ=255)
            aCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              >  3:964161/1‑134 (MQ=255)
            aCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              <  4:964161/134‑1 (MQ=255)
               ggTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGa                                                                                                                                              >  4:273376/1‑131 (MQ=255)
               ggTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGa                                                                                                                                              <  3:273376/131‑1 (MQ=255)
                      aTCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcc                                                                                                                      <  2:1010116/148‑1 (MQ=255)
                        cAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcccc                                                                                                                    >  4:613636/1‑148 (MQ=255)
                          aTGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCgg                                                                                                                  >  2:328593/1‑148 (MQ=255)
                          aTGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCgg                                                                                                                  >  1:289852/1‑148 (MQ=255)
                           tGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              <  2:110211/119‑1 (MQ=255)
                           tGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              >  1:110211/1‑119 (MQ=255)
                            gCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGtt                                                                                                                >  2:1115902/1‑148 (MQ=255)
                              cTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTa                                                                                                              <  2:289852/148‑1 (MQ=255)
                               tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatcat                                                                                                                           >  2:733969/1‑134 (MQ=255)
                               tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatca                                                                                                                            >  3:221671/1‑133 (MQ=255)
                               tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatca                                                                                                                            <  4:221671/133‑1 (MQ=255)
                               tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             >  1:247188/1‑148 (MQ=255)
                               tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             <  2:247188/148‑1 (MQ=255)
                               tGCGCGAGCGGTGAGAACGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatcat                                                                                                                           <  1:733969/134‑1 (MQ=255)
                                gcgcGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatca                                                                                                                            <  3:925061/132‑1 (MQ=255)
                                gcgcGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatca                                                                                                                            >  4:925061/1‑132 (MQ=255)
                                  gcgAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAg                                                                                                          <  3:613636/148‑1 (MQ=255)
                                       cGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaa                                                                                                     <  1:944571/148‑1 (MQ=255)
                                       cGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaa                                                                                                     <  1:1115902/148‑1 (MQ=255)
                                                    ccGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGt                                                                                        >  3:308139/1‑148 (MQ=255)
                                                       tCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACt                                                                                                                                        <  1:386315/97‑1 (MQ=255)
                                                       tCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACt                                                                                                                                        >  2:386315/1‑97 (MQ=255)
                                                          aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATc                                                                                                                                    >  4:344347/1‑98 (MQ=255)
                                                          aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATc                                                                                                                                    <  3:344347/98‑1 (MQ=255)
                                                          aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  1:1024416/1‑133 (MQ=255)
                                                          aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 <  2:1024416/133‑1 (MQ=255)
                                                          aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGt                                                                                  >  1:1045758/1‑148 (MQ=255)
                                                           tCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    >  2:867411/1‑129 (MQ=255)
                                                           tCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    >  2:106702/1‑129 (MQ=255)
                                                           tCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  1:106702/129‑1 (MQ=255)
                                                           tCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  1:867411/129‑1 (MQ=255)
                                                              cTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGt                                                                              <  1:328593/148‑1 (MQ=255)
                                                                tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            <  1:368324/116‑1 (MQ=255)
                                                                tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCAACGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            >  2:368324/1‑116 (MQ=255)
                                                                tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              >  2:1119715/1‑82 (MQ=255)
                                                                tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              <  1:1119715/82‑1 (MQ=255)
                                                                 cGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGt                                                                                        <  4:1108973/135‑1 (MQ=255)
                                                                 cGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGt                                                                                        >  3:1108973/1‑135 (MQ=255)
                                                                   aCAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCca                                                                         >  4:555416/1‑148 (MQ=255)
                                                                      aTCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  1:974067/1‑121 (MQ=255)
                                                                      aTCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 <  2:974067/121‑1 (MQ=255)
                                                                        cAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            >  4:979438/1‑108 (MQ=255)
                                                                        cAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            <  3:979438/108‑1 (MQ=255)
                                                                           ttCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcc                                                                                                                      >  1:570251/1‑95 (MQ=255)
                                                                           ttCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcc                                                                                                                      <  2:570251/95‑1 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 <  4:570400/110‑1 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  3:651959/1‑110 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  1:1029545/1‑110 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 >  3:570400/1‑110 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 <  2:1029545/110‑1 (MQ=255)
                                                                                 cATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCa                                                                                                 <  4:651959/110‑1 (MQ=255)
                                                                                  aTCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGa                                                                                                           >  2:620046/1‑99 (MQ=255)
                                                                                  aTCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGa                                                                                                           <  1:620046/99‑1 (MQ=255)
                                                                                  aTCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGa                                                          >  3:232945/1‑148 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGc                                                                                                                                           <  2:696132/66‑1 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGc                                                                                                                                           >  1:696132/1‑66 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            <  4:567010/97‑1 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATg                                                                                                            >  3:567010/1‑97 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaa                                                                                                     <  2:1052745/104‑1 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaa                                                                                                     >  1:1052745/1‑104 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGAc                                                         >  1:1021220/1‑148 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              <  3:568749/63‑1 (MQ=255)
                                                                                   tCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAGGCACCGa                                                                                                                                              >  4:568749/1‑63 (MQ=255)
                                                                                     cgccgGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGt                                                       <  4:308139/148‑1 (MQ=255)
                                                                                          ggTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCAt                                                                                                                                     >  3:126574/1‑65 (MQ=255)
                                                                                          ggTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCAt                                                                                                                                     <  4:126574/65‑1 (MQ=255)
                                                                                                   cACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTg                                         <  3:555416/148‑1 (MQ=255)
                                                                                                        gCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGttt                                                                            >  3:817742/1‑108 (MQ=255)
                                                                                                        gCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGttt                                                                            <  4:817742/108‑1 (MQ=255)
                                                                                                         cTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTg                                   <  4:232945/148‑1 (MQ=255)
                                                                                                            cgcgcCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGAtt                                >  2:1037968/1‑148 (MQ=255)
                                                                                                                ccGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTc                            <  2:1045758/148‑1 (MQ=255)
                                                                                                                    tCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagc                                                                       <  4:941571/101‑1 (MQ=255)
                                                                                                                    tCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagc                                                                       >  3:941571/1‑101 (MQ=255)
                                                                                                                      aGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCAt                      <  1:1037968/148‑1 (MQ=255)
                                                                                                                        cGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTcc                                                                          <  2:252493/94‑1 (MQ=255)
                                                                                                                        cGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTcc                                                                          >  1:252493/1‑94 (MQ=255)
                                                                                                                        cGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCg                    >  3:991608/1‑148 (MQ=255)
                                                                                                                             gggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAg               <  4:991608/148‑1 (MQ=255)
                                                                                                                                cGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGc            >  4:689625/1‑148 (MQ=255)
                                                                                                                                   cGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGttt                                                                            <  1:740950/81‑1 (MQ=255)
                                                                                                                                   cGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGttt                                                                            >  2:740950/1‑81 (MQ=255)
                                                                                                                                      tGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGt                                                                                        <  4:516810/66‑1 (MQ=255)
                                                                                                                                      tGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGt                                                                                        >  3:516810/1‑66 (MQ=255)
                                                                                                                                      tGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGt          >  1:784289/1‑144 (MQ=255)
                                                                                                                                      tGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGt          <  2:784289/144‑1 (MQ=255)
                                                                                                                                          tGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTcgcg  >  2:1108151/1‑148 (MQ=255)
                                                                                                                                          tGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTcgcg  <  1:1108151/148‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566129‑2566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: