| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,592,989 | A→G | 13.0% | intergenic (+27/+83) | ypfN → / ← ypfH | putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,592,989 | 0 | A | G | 13.0% | 127.6 / 10.7 | 46 | intergenic (+27/+83) | ypfN/ypfH | putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base G (3/3); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01 | |||||||||||
AGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCAT > NC_000913/2592844‑2593094 | aGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg < 1:654751/148‑1 (MQ=255) cgcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg < 1:1027049/147‑1 (MQ=255) cgcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg > 2:1027049/1‑147 (MQ=255) aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt > 2:909416/1‑137 (MQ=255) aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGGGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGt < 1:909416/137‑1 (MQ=255) gAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc > 2:94012/1‑148 (MQ=255) cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg < 1:94012/148‑1 (MQ=255) caaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctt > 1:843752/1‑128 (MQ=255) caaGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctt < 2:843752/130‑3 (MQ=255) ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc > 4:466228/1‑148 (MQ=255) ccGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc < 3:466228/148‑1 (MQ=255) aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg < 3:125938/110‑1 (MQ=255) aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAgg > 4:125938/1‑110 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCg > 3:144015/1‑105 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCg < 4:144015/105‑1 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg > 4:501030/1‑111 (MQ=255) cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg < 3:501030/111‑1 (MQ=255) ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgccttatc < 3:619800/117‑6 (MQ=255) ccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgccttatc > 4:619800/1‑112 (MQ=255) cAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATc > 2:331378/1‑148 (MQ=255) ctAAGTGGCACGATGACGACGACTGGCCGAACCCGTCTCAACCGAAGAAGTAAGCCGAGTGCTCAATCGTAGGCAGGATAAGGCGTTCGCGCGGCAACCGGCAATGGTGAAAGAAACCCAATGAAACGCTCCCGCGTCCTATCCGGcc < 4:520941/147‑1 (MQ=255) aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATg > 2:975942/1‑116 (MQ=255) aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATg < 1:975942/116‑1 (MQ=255) gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg < 1:505065/76‑1 (MQ=255) gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg > 2:505065/1‑76 (MQ=255) gACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg < 3:1066427/126‑1 (MQ=255) gACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg > 4:1066427/1‑126 (MQ=255) cGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTCAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg < 1:331378/148‑1 (MQ=255) ggCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa > 3:360037/1‑86 (MQ=255) ggCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa < 4:360037/86‑1 (MQ=255) cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg < 3:468507/80‑1 (MQ=255) cGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg > 4:468507/1‑80 (MQ=255) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctta < 4:898689/67‑4 (MQ=37) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAAcgcctta > 3:898689/1‑64 (MQ=37) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc < 4:1005702/69‑1 (MQ=255) aaaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc > 3:1005702/1‑69 (MQ=255) aaaaGGATCAACCGAAGATGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa > 4:717657/1‑74 (MQ=255) aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa < 3:717657/74‑1 (MQ=255) agaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgc < 3:70713/47‑1 (MQ=255) agaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgc > 4:70713/1‑47 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt > 4:1093707/1‑93 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTAt < 3:1093707/93‑1 (MQ=255) aCCGTATGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg > 2:612003/1‑81 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAgg < 1:612003/81‑1 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcat < 2:1039169/120‑1 (MQ=255) aCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcat > 1:1039169/1‑120 (MQ=255) | AGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCAT > NC_000913/2592844‑2593094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |