Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,620,623 T→C 5.5% M84V (ATG→GTG)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,620,6230TC 5.5% 245.0 / 3.4 72M84V (ATG→GTG) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base C (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GTCAACGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGC  >  NC_000913/2620483‑2620763
                                                                                                                                            |                                                                                                                                            
gTCAACGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCa                                                                                                                                       <  4:1018186/148‑1 (MQ=255)
   aaCGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             >  4:370674/1‑139 (MQ=255)
   aaCGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             <  3:370674/139‑1 (MQ=255)
    aCGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGAc                                                                                                                                   >  2:1051315/1‑148 (MQ=255)
       aTCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatcat                                                                                                                                           <  3:1089486/137‑1 (MQ=255)
       aTCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatcat                                                                                                                                           >  4:1089486/1‑137 (MQ=255)
       aTCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             >  3:155507/1‑135 (MQ=255)
       aTCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             <  4:155507/135‑1 (MQ=255)
               cATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGa                                                                                                                        <  2:68664/148‑1 (MQ=255)
                 tACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAAt                                                                                                                      >  4:569755/1‑148 (MQ=255)
                   cGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTg                                                                                                                    <  1:1051315/148‑1 (MQ=255)
                   cGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTg                                                                                                                    <  1:471118/148‑1 (MQ=255)
                           gATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACg                                                                                                            <  3:569755/148‑1 (MQ=255)
                                tAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAAt                                                                                                                      <  3:663416/133‑1 (MQ=255)
                                tAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAAt                                                                                                                      >  4:663416/1‑133 (MQ=255)
                                 aGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             <  1:390409/109‑1 (MQ=255)
                                 aGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCcatc                                                                                                                                             >  2:390409/1‑109 (MQ=255)
                                  gacaCCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAAttt                                                                                                     >  2:867155/4‑148 (MQ=255)
                                  gAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTgg                                                                                                                   >  1:981742/1‑134 (MQ=255)
                                  gAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTgg                                                                                                                   <  2:981742/134‑1 (MQ=255)
                                  gAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAAttt                                                                                                     >  1:503922/1‑148 (MQ=255)
                                     aCCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCt                                                                                                  >  3:809935/1‑148 (MQ=255)
                                      ccAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCtt                                                                                                 <  4:809935/148‑1 (MQ=255)
                                       cAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTa                                                                                                <  2:503922/148‑1 (MQ=255)
                                             cTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTg                                                                                          <  1:867155/148‑1 (MQ=255)
                                              tGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCcg                                                                                                                                >  1:340057/1‑109 (MQ=255)
                                              tGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCcg                                                                                                                                <  2:340057/109‑1 (MQ=255)
                                                 aaGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGAcc                                                                                                                                  >  1:1080453/1‑104 (MQ=255)
                                                 aaGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGAcc                                                                                                                                  <  2:1080453/104‑1 (MQ=255)
                                                  aGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTg                                                                                     >  2:865504/1‑148 (MQ=255)
                                                    tACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTc                                                                                                   <  3:498798/132‑1 (MQ=255)
                                                    tACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTc                                                                                                   >  4:498798/1‑132 (MQ=255)
                                                         tACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGc                                                                                                                  >  4:572050/1‑112 (MQ=255)
                                                         tACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGc                                                                                                                  <  3:572050/112‑1 (MQ=255)
                                                                 ccAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCgg                                                                      <  1:865504/148‑1 (MQ=255)
                                                                  cAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCggg                                                                     <  2:617789/148‑1 (MQ=255)
                                                                   aGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGaa                                                                                                                       <  4:606858/97‑1 (MQ=255)
                                                                   aGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGaa                                                                                                                       >  3:606858/1‑97 (MQ=255)
                                                                    gCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCttt                                                                                           <  1:826738/124‑1 (MQ=255)
                                                                    gCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCttt                                                                                           >  2:826738/1‑124 (MQ=255)
                                                                            tcATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGc                                                           >  4:10164/1‑148 (MQ=255)
                                                                                 aCGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCg                                                      <  3:10164/148‑1 (MQ=255)
                                                                                      aCATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    <  3:553420/145‑1 (MQ=255)
                                                                                      aCATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    >  4:553420/1‑145 (MQ=255)
                                                                                            cGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGt                                                                                   >  1:543908/1‑108 (MQ=255)
                                                                                            cGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGt                                                                                   <  2:543908/108‑1 (MQ=255)
                                                                                            cGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTcc                                                              <  1:631459/129‑1 (MQ=255)
                                                                                            cGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTcc                                                              >  2:631459/1‑129 (MQ=255)
                                                                                               cAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCtt                                                                                                 <  1:406783/91‑1 (MQ=255)
                                                                                               cAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCtt                                                                                                 >  2:406783/1‑91 (MQ=255)
                                                                                                aaCGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTcc                                       >  4:130956/1‑148 (MQ=255)
                                                                                                      gATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCg                                                      >  1:353864/1‑127 (MQ=255)
                                                                                                      gATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCg                                                      <  2:353864/127‑1 (MQ=255)
                                                                                                       aTGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCg                                                      >  2:770340/1‑126 (MQ=255)
                                                                                                       aTGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCg                                                      <  1:770340/126‑1 (MQ=255)
                                                                                                          cgcgcgcTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTgg                                                                                    >  1:281491/1‑93 (MQ=255)
                                                                                                          cgcgcgcTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTgg                                                                                    <  2:281491/93‑1 (MQ=255)
                                                                                                              cgcTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTa                                                                                                         <  4:625936/68‑1 (MQ=255)
                                                                                                              cgcTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTa                                                                                                         >  3:625936/1‑68 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTa                                                                          >  4:386029/1‑93 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTa                                                                          <  1:192654/93‑1 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTa                                                                          <  3:386029/93‑1 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTa                                                                          >  2:192654/1‑93 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGt                                     >  4:1017598/1‑130 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGt                                     >  4:1035316/1‑130 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGt                                     <  3:1017598/130‑1 (MQ=255)
                                                                                                                    gAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGt                                     <  3:1035316/130‑1 (MQ=255)
                                                                                                                     aaCGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCg                                 <  1:797834/133‑1 (MQ=255)
                                                                                                                     aaCGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCg                                 >  2:797834/1‑133 (MQ=255)
                                                                                                                       cGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCtt                 <  4:88067/147‑1 (MQ=255)
                                                                                                                       cGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCtt                 >  3:88067/1‑147 (MQ=255)
                                                                                                                          tttCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGAt                                                                               >  1:819238/1‑82 (MQ=255)
                                                                                                                          tttCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGAt                                                                               <  2:819238/82‑1 (MQ=255)
                                                                                                                                gCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCt                                                         <  4:30101/98‑1 (MQ=255)
                                                                                                                                gCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCt                                                         >  3:30101/1‑98 (MQ=255)
                                                                                                                                gCACACCGTCCACCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    >  4:338531/1‑103 (MQ=255)
                                                                                                                                gCACACCGTCCACCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    <  3:338531/103‑1 (MQ=255)
                                                                                                                                gCACACCGTCCACCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    <  1:838747/103‑1 (MQ=255)
                                                                                                                                gCACACCGTCCACCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGAt                                                    >  2:838747/1‑103 (MQ=255)
                                                                                                                                     ccGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGc  >  2:944925/1‑148 (MQ=255)
                                                                                                                                            tcatACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCg                                                                  >  1:211477/1‑77 (MQ=255)
                                                                                                                                            tcatACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCg                                                                  <  2:211477/77‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GTCAACGATCAGCGCCATACGCTCATCGATGTTAGAAACCAGTTTCTGGAAGTACGGTACCGGCTCCAGCGTTTCTTCATTACGGTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACACCGTCCATCATACCAAGACCCGCACGCAGAATTGGCACAACGGTAATTTTCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGC  >  NC_000913/2620483‑2620763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑