| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,632,035 | A→T | 8.3% | I167I (ATT→ATA) | guaA ← | GMP synthetase (glutamine aminotransferase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,632,035 | 0 | A | T | 8.3% | 207.2 / 8.7 | 60 | I167I (ATT→ATA) | guaA | GMP synthetase (glutamine aminotransferase) |
| Reads supporting (aligned to +/- strand): ref base A (29/26); new base T (3/2); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
TCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCT > NC_000913/2631911‑2632181 | tCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAAc > 2:63062/1‑134 (MQ=255)tCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAAc < 1:63062/134‑1 (MQ=255) caGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATg < 3:617425/124‑1 (MQ=255) caGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATg > 4:617425/1‑124 (MQ=255) caGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTg > 3:131253/1‑148 (MQ=255) ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATa > 1:44362/1‑118 (MQ=255) ggCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATa < 2:44362/118‑1 (MQ=255) ttCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACg < 4:131253/148‑1 (MQ=255) cacTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTTGCCATTATgg > 3:894952/1‑115 (MQ=255) cacTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTTGCCATTATgg < 4:894952/115‑1 (MQ=255) acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCt > 1:373443/1‑136 (MQ=255) acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCt < 2:373443/136‑1 (MQ=255) cAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGt > 3:559957/1‑148 (MQ=255) cacaAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCg < 3:83933/115‑1 (MQ=255) cacaAAACGCTCCAGCACGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCCAACGGGCAGATTTCg > 4:83933/1‑115 (MQ=255) aaaCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGtg < 2:255588/126‑1 (MQ=255) aaaCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGtg > 1:255588/1‑126 (MQ=255) aCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTa < 4:559957/148‑1 (MQ=255) cccgccagggTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCa > 4:583102/8‑148 (MQ=255) tGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACg < 2:161497/99‑1 (MQ=255) tGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACg > 1:161497/1‑99 (MQ=255) gTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACAt < 3:583102/148‑1 (MQ=255) tATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTaa > 1:876085/1‑120 (MQ=255) tATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTaa < 2:876085/120‑1 (MQ=255) aTGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTACCTTTATCGCCGTGGCTCATc < 4:370476/139‑1 (MQ=255) aTGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATc > 3:370476/1‑139 (MQ=255) aGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAAt < 2:113648/108‑1 (MQ=255) aGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAAt > 1:113648/1‑108 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCt > 3:1056039/1‑78 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCt < 4:1056039/78‑1 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGc > 1:548489/1‑108 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGc < 2:548489/108‑1 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCgg < 3:929156/110‑1 (MQ=255) cACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCgg > 4:929156/1‑110 (MQ=255) aCTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGt > 4:318718/1‑81 (MQ=255) aCTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGt < 3:318718/81‑1 (MQ=255) aGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATc > 2:181953/1‑148 (MQ=255) gAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCt < 1:181953/148‑1 (MQ=255) gTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGt < 4:560591/133‑1 (MQ=255) gTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGt > 3:560591/1‑133 (MQ=255) ttttCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGa < 4:137455/109‑1 (MQ=255) ttttCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGa > 3:137455/1‑109 (MQ=255) tcGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg < 4:1054521/87‑1 (MQ=255) tcGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTgg > 3:1054521/1‑87 (MQ=255) tcGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTg > 1:1082947/1‑86 (MQ=255) tcGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTg < 2:1082947/86‑1 (MQ=255) ccGTTTGCCATTATGGCTAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTAc > 4:1109661/2‑115 (MQ=255) ccGTTTGCCATTATGGCTAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTAc < 3:1109661/114‑1 (MQ=255) cGTTTGCCATTATGGCTAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAAc > 1:809472/1‑148 (MQ=255) tAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCtt < 1:600566/132‑1 (MQ=255) tAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCtt > 2:600566/1‑132 (MQ=255) tAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACcg > 4:1031845/1‑140 (MQ=255) tAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACcg < 3:1031845/140‑1 (MQ=255) gCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTg > 2:226360/1‑148 (MQ=255) ccATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagcagc < 4:1129524/101‑1 (MQ=255) ccATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagcagc > 3:1129524/1‑101 (MQ=255) cATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCg < 1:1113988/94‑1 (MQ=255) cATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCg > 2:1113988/1‑94 (MQ=255) cATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagcag > 4:501981/1‑99 (MQ=255) cATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagcag < 3:501981/99‑1 (MQ=255) cATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTgcg > 3:63267/1‑148 (MQ=255) tAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCt > 4:635463/1‑148 (MQ=255) aaTGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACa > 4:407170/1‑88 (MQ=255) aaTGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACa < 3:407170/88‑1 (MQ=255) | TCCACAGGGCTTCACACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGCGCT > NC_000913/2631911‑2632181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |