Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,372 T→C 36.4% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3720TC36.4% 17.5 / 20.1 22intergenic (+33/‑483)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (7/7);  new base C (4/4);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

TTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCA  >  NC_000913/2653233‑2653489
                                                                                                                                           |                                                                                                                     
ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGtt                                                                                                                >  4:832107/1‑147 (MQ=255)
ttttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGtt                                                                                                                <  3:832107/147‑1 (MQ=255)
                                aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                      >  3:599724/1‑109 (MQ=255)
                                aaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGAAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                      <  4:599724/109‑1 (MQ=255)
                                                              gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                <  2:775596/85‑1 (MQ=255)
                                                              gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                >  1:775596/1‑85 (MQ=255)
                                                                                  tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                       >  4:206616/1‑58 (MQ=255)
                                                                                  tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                       <  3:206616/58‑1 (MQ=255)
                                                                                                                         aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                <  4:575511/90‑1 (MQ=255)
                                                                                                                         aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCCGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                >  3:575511/1‑90 (MQ=255)
                                                                                                                               aagcgccaAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                                        <  3:831782/89‑1 (MQ=37)
                                                                                                                               aaCCGCCAAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTca                                        >  4:831782/1‑92 (MQ=37)
                                                                                                                                aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                                                 <  1:947307/82‑1 (MQ=255)
                                                                                                                                aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                                                 >  2:947307/1‑82 (MQ=255)
                                                                                                                                aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt       <  2:188210/124‑1 (MQ=255)
                                                                                                                                aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt       >  1:188210/1‑124 (MQ=255)
                                                                                                                                aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCa  >  2:238520/1‑129 (MQ=255)
                                                                                                                                aCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCa  <  1:238520/129‑1 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTAc                                                             >  2:202752/1‑66 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTAc                                                             <  1:202752/66‑1 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                 <  2:751229/94‑1 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                 >  1:751229/1‑94 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt       >  1:175802/1‑120 (MQ=255)
                                                                                                                                    ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt       <  2:175802/120‑1 (MQ=255)
                                                                                                                                           |                                                                                                                     
TTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCA  >  NC_000913/2653233‑2653489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: