| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,703,331 | A→T | 6.0% | V19D (GTT→GAT) | era ← | membrane‑associated, 16S rRNA‑binding GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,703,331 | 0 | A | T | 6.0% | 237.7 / 5.6 | 67 | V19D (GTT→GAT) | era | membrane‑associated, 16S rRNA‑binding GTPase |
| Reads supporting (aligned to +/- strand): ref base A (31/32); new base T (2/2); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-01 | |||||||||||
TATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTG > NC_000913/2703185‑2703455 | tatGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAAc > 2:1094215/1‑148 (MQ=255) gCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGtt < 1:914915/148‑1 (MQ=255) cAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTc < 1:1094215/148‑1 (MQ=255) cGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGt > 3:974209/1‑148 (MQ=255) cGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGAt < 4:974209/148‑1 (MQ=255) aCGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCgg < 2:339013/134‑1 (MQ=255) aCGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCgg > 1:339013/1‑134 (MQ=255) tAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCg < 4:667194/130‑1 (MQ=255) tAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCg > 3:667194/1‑130 (MQ=255) tAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcg < 1:1043450/138‑1 (MQ=255) tAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcg > 2:1043450/1‑138 (MQ=255) tAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCaa > 2:120657/1‑148 (MQ=255) gATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg < 4:937637/139‑1 (MQ=255) gATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg > 3:937637/1‑139 (MQ=255) tCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATaaa < 1:142600/148‑1 (MQ=255) tCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATaaa > 2:142600/1‑148 (MQ=255) cGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcga < 2:385515/134‑1 (MQ=255) cGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcga > 1:385515/1‑134 (MQ=255) cTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCATCG‑TCGGACGTCcgaacgtt < 2:416028/135‑6 (MQ=255) cTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGACTTGCCATCG‑TCGGACGTCcgaacgtt > 1:416028/1‑130 (MQ=255) cgcCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCATCG‑TCGGACGTCcgaacgtt < 4:1017139/127‑6 (MQ=255) cgcCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCATCG‑TCGGACGTCcgaacgtt > 3:1017139/1‑122 (MQ=255) cTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACtttt < 1:120657/148‑1 (MQ=255) aTGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCg > 1:849904/1‑145 (MQ=255) aTGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCg < 2:849904/145‑1 (MQ=255) tCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCAt > 1:491729/1‑148 (MQ=255) ccccACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGt > 3:338163/1‑103 (MQ=255) ccccACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGt < 4:338163/103‑1 (MQ=255) ccACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACttt < 3:886077/132‑1 (MQ=255) ccACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACttt > 4:886077/1‑132 (MQ=255) cacaATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCgg > 3:425763/1‑96 (MQ=255) cacaATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCgg < 4:425763/96‑1 (MQ=255) cacaATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGc < 2:876622/122‑1 (MQ=255) cacaATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGc > 1:876622/1‑122 (MQ=255) ggTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGCTGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGt < 2:491729/148‑1 (MQ=255) cTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCaa > 1:1035054/1‑137 (MQ=255) cTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCaa < 2:1035054/137‑1 (MQ=255) ttGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTa > 2:181293/1‑105 (MQ=255) ttGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTa < 1:181293/105‑1 (MQ=255) tGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGt > 2:120100/1‑71 (MQ=255) tGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGt < 1:120100/71‑1 (MQ=255) tGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGAt > 1:629677/1‑79 (MQ=255) tGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGAt < 2:629677/79‑1 (MQ=255) gagaTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCGGCTCCAGttt > 1:779294/1‑115 (MQ=255) gagaTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGttt < 2:779294/115‑1 (MQ=255) agaTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCggcggc > 3:1028463/1‑135 (MQ=255) agaTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCggcggc < 4:1028463/135‑1 (MQ=255) aTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACtttt > 2:848023/1‑86 (MQ=255) aTTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACtttt < 1:848023/86‑1 (MQ=255) tttCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCaa < 3:918687/116‑1 (MQ=255) tttCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCaa > 4:918687/1‑116 (MQ=255) tttCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAg < 3:339771/133‑1 (MQ=255) tttCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAg > 4:339771/1‑133 (MQ=255) ccAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTa < 2:663359/70‑1 (MQ=255) ccAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTa > 1:663359/1‑70 (MQ=255) agcagTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTg > 3:33928/1‑127 (MQ=255) agcagTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTg < 4:33928/127‑1 (MQ=255) aaTGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCt > 2:922634/1‑120 (MQ=255) aaTGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCt < 1:922634/120‑1 (MQ=255) ggATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGc < 4:108466/124‑1 (MQ=255) ggATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGc > 3:108466/1‑124 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTc < 4:146767/66‑1 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTc > 3:146767/1‑66 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCggcggc > 2:10502/1‑93 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCggcggc < 1:10502/93‑1 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTa > 2:209054/1‑109 (MQ=255) cAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTa < 1:209054/109‑1 (MQ=255) aCGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCtgtg < 2:987396/125‑1 (MQ=255) aCGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCtgtg > 1:987396/1‑125 (MQ=255) | TATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAGTAACTTTTATCGATGCTCATTCCAGCTCCAGTTTTTTCAACGCCTGTTCGGCGGCAGCCTGCTCAGCCTTACGACGGCTTGAACCTGTG > NC_000913/2703185‑2703455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |