Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,744,136 A→G 5.9% intergenic (+29/+47) yfiB → / ← rplS OM lipoprotein putative positive effector of YfiN activity/50S ribosomal subunit protein L19

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,744,1360AG 5.9% 231.7 / 3.0 68intergenic (+29/+47)yfiB/rplSOM lipoprotein putative positive effector of YfiN activity/50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base A (31/33);  new base G (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGAC  >  NC_000913/2743990‑2744275
                                                                                                                                                  |                                                                                                                                           
tCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGgccag                                                                                                                                            >  4:79895/1‑148 (MQ=255)
tCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGgccag                                                                                                                                            <  1:30352/148‑1 (MQ=255)
                 ccacACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                           >  3:346103/1‑148 (MQ=255)
                  cacacaGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttc                                                                                                                          <  4:346103/148‑1 (MQ=255)
                    cacaGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttctt                                                                                                                        >  3:79475/1‑148 (MQ=255)
                        gggTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                    <  4:79475/148‑1 (MQ=255)
                                             cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGgccagc                                                                                                                                           >  4:221820/1‑104 (MQ=255)
                                             cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGgccagc                                                                                                                                           <  3:221820/104‑1 (MQ=255)
                                             cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                                                      >  3:790397/1‑125 (MQ=255)
                                             cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                                                      <  4:790397/125‑1 (MQ=255)
                                              aTAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAAc                                                                                                                     <  3:622873/125‑1 (MQ=255)
                                              aTAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAAc                                                                                                                     >  4:622873/1‑125 (MQ=255)
                                                      tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGccc                                                                                                                             <  1:520329/109‑1 (MQ=255)
                                                      tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGccc                                                                                                                             >  2:520329/1‑109 (MQ=255)
                                                      tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGCAATCTTAGTTAAg                                                                                      <  3:79895/148‑1 (MQ=255)
                                                           aGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCTCTTAAGCGAAATCtt                                                                                             <  4:550948/136‑1 (MQ=255)
                                                           aGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                             >  3:550948/1‑136 (MQ=255)
                                                             aCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttct                                                                                                                         <  4:362709/106‑1 (MQ=255)
                                                             aCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttct                                                                                                                         >  3:362709/1‑106 (MQ=255)
                                                                     gggCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAGGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                    >  4:935195/1‑103 (MQ=255)
                                                                     gggCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                    <  3:935195/103‑1 (MQ=255)
                                                                                 gaACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                             >  4:826256/1‑114 (MQ=255)
                                                                                 gaACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                             <  3:826256/114‑1 (MQ=255)
                                                                                   ctcggcgcgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAg                                                         <  4:824577/143‑1 (MQ=255)
                                                                                          cgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCTAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                        <  4:140208/94‑1 (MQ=255)
                                                                                          cgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCTAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                        >  3:140208/1‑94 (MQ=255)
                                                                                                  tggtgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGc                                                                                                           >  2:296889/1‑83 (MQ=255)
                                                                                                  tggtgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGc                                                                                                           <  1:296889/83‑1 (MQ=255)
                                                                                                     tgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAg                                                                                                                   >  3:1058952/1‑72 (MQ=255)
                                                                                                     tgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAg                                                                                                                   <  4:1058952/72‑1 (MQ=255)
                                                                                                      gATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAg                                                                                           <  3:1121038/95‑1 (MQ=255)
                                                                                                      gATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAg                                                                                           >  4:1121038/1‑95 (MQ=255)
                                                                                                            tacCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                        >  3:563747/1‑76 (MQ=255)
                                                                                                            tacCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                        <  4:563747/76‑1 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAgg                                                                                                                  <  3:121749/65‑1 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAgg                                                                                                                  >  4:121749/1‑65 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAg                                                                                      <  1:364624/93‑1 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAg                                                                                      >  2:364624/1‑93 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                            <  1:645377/119‑1 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                            >  4:1139556/1‑119 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                            <  3:1139556/119‑1 (MQ=255)
                                                                                                             acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                            >  2:645377/1‑119 (MQ=255)
                                                                                                               cccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                               >  2:744575/1‑146 (MQ=255)
                                                                                                               cccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                               <  1:744575/146‑1 (MQ=255)
                                                                                                                ccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                           >  2:866498/1‑53 (MQ=255)
                                                                                                                ccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                           <  1:866498/53‑1 (MQ=255)
                                                                                                                 cTTAAAACACCAGCAAACAGATAAAAAAGGgccag                                                                                                                                            >  3:443768/1‑35 (MQ=255)
                                                                                                                 cTTAAAACACCAGCAAACAGATAAAAAAGGgccag                                                                                                                                            <  4:443768/35‑1 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                             >  3:193234/1‑113 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                             <  4:193234/113‑1 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGt                                                        >  3:221094/1‑118 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGt                                                        <  4:221094/118‑1 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgt                                          <  4:477567/132‑1 (MQ=255)
                                                                                                                  ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCCTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgt                                          >  3:477567/1‑132 (MQ=255)
                                                                                                                   tAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACg                                                                       >  4:302861/1‑102 (MQ=255)
                                                                                                                   tAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACg                                                                       <  3:302861/102‑1 (MQ=255)
                                                                                                                     aaaCACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATg                                                                                                               >  2:565422/1‑60 (MQ=255)
                                                                                                                     aaaCACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATg                                                                                                               <  1:565422/60‑1 (MQ=255)
                                                                                                                       acacCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATa                                                                         <  3:548600/96‑1 (MQ=255)
                                                                                                                       acacCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATa                                                                         >  4:548600/1‑96 (MQ=255)
                                                                                                                          ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                               >  1:501192/1‑135 (MQ=255)
                                                                                                                          ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                               <  2:501192/135‑1 (MQ=255)
                                                                                                                          ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGaa                     <  1:159539/145‑1 (MQ=255)
                                                                                                                          ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGaa                     >  2:159539/1‑145 (MQ=255)
                                                                                                                                    gATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTg                                                                            >  1:497332/1‑80 (MQ=255)
                                                                                                                                    gATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTg                                                                            <  2:497332/80‑1 (MQ=255)
                                                                                                                                       aaaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTa                                                                                            >  1:667499/1‑61 (MQ=255)
                                                                                                                                       aaaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTa                                                                                            <  2:667499/61‑1 (MQ=255)
                                                                                                                                        aaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCa                                                          <  1:597584/94‑1 (MQ=255)
                                                                                                                                        aaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCa                                                          >  2:597584/1‑94 (MQ=255)
                                                                                                                                          gaaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGAc  >  4:30718/2‑148 (MQ=255)
                                                                                                                                           aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                         >  1:484345/1‑124 (MQ=255)
                                                                                                                                           aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                         <  2:484345/124‑1 (MQ=255)
                                                                                                                                           aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGa                      <  2:124006/127‑1 (MQ=255)
                                                                                                                                                  agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                             <  3:866964/81‑1 (MQ=255)
                                                                                                                                                  agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                             >  4:866964/1‑81 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
TCCACGCAGCAATCTCACCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGAC  >  NC_000913/2743990‑2744275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: