Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,744,148 A→G 6.0% intergenic (+41/+35) yfiB → / ← rplS OM lipoprotein putative positive effector of YfiN activity/50S ribosomal subunit protein L19

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,744,1480AG 6.0% 288.5 / 5.2 83intergenic (+41/+35)yfiB/rplSOM lipoprotein putative positive effector of YfiN activity/50S ribosomal subunit protein L19
Reads supporting (aligned to +/- strand):  ref base A (38/40);  new base G (3/2);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCG  >  NC_000913/2744007‑2744295
                                                                                                                                             |                                                                                                                                                   
ccacACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                                               >  3:346103/1‑148 (MQ=255)
 cacacaGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttc                                                                                                                                              <  4:346103/148‑1 (MQ=255)
   cacaGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttctt                                                                                                                                            >  3:79475/1‑148 (MQ=255)
       gggTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                                        <  4:79475/148‑1 (MQ=255)
                            cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                                                                          >  3:790397/1‑125 (MQ=255)
                            cATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTaa                                                                                                                                          <  4:790397/125‑1 (MQ=255)
                             aTAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAAc                                                                                                                                         <  3:622873/125‑1 (MQ=255)
                             aTAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAAc                                                                                                                                         >  4:622873/1‑125 (MQ=255)
                                     tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGccc                                                                                                                                                 <  1:520329/109‑1 (MQ=255)
                                     tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGccc                                                                                                                                                 >  2:520329/1‑109 (MQ=255)
                                     tAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGCAATCTTAGTTAAg                                                                                                          <  3:79895/148‑1 (MQ=255)
                                          aGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCTCTTAAGCGAAATCtt                                                                                                                 <  4:550948/136‑1 (MQ=255)
                                          aGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                                                 >  3:550948/1‑136 (MQ=255)
                                            aCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttct                                                                                                                                             <  4:362709/106‑1 (MQ=255)
                                            aCCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCcttct                                                                                                                                             >  3:362709/1‑106 (MQ=255)
                                                    gggCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAGGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                                        >  4:935195/1‑103 (MQ=255)
                                                    gggCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACa                                                                                                                                        <  3:935195/103‑1 (MQ=255)
                                                                gaACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                                                 <  3:826256/114‑1 (MQ=255)
                                                                gaACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCtt                                                                                                                 >  4:826256/1‑114 (MQ=255)
                                                                  ctcggcgcgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAg                                                                             <  4:824577/143‑1 (MQ=255)
                                                                         cgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCTAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                                            >  3:140208/1‑94 (MQ=255)
                                                                         cgTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCTAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                                            <  4:140208/94‑1 (MQ=255)
                                                                                 tggtgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGc                                                                                                                               >  2:296889/1‑83 (MQ=255)
                                                                                 tggtgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGc                                                                                                                               <  1:296889/83‑1 (MQ=255)
                                                                                    tgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAg                                                                                                                                       >  3:1058952/1‑72 (MQ=255)
                                                                                    tgATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAg                                                                                                                                       <  4:1058952/72‑1 (MQ=255)
                                                                                     gATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAg                                                                                                               >  4:1121038/1‑95 (MQ=255)
                                                                                     gATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAg                                                                                                               <  3:1121038/95‑1 (MQ=255)
                                                                                           tacCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                                            <  4:563747/76‑1 (MQ=255)
                                                                                           tacCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTa                                                                                                                            >  3:563747/1‑76 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAgg                                                                                                                                      >  4:121749/1‑65 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAgg                                                                                                                                      <  3:121749/65‑1 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAg                                                                                                          <  1:364624/93‑1 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAg                                                                                                          >  2:364624/1‑93 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                                                <  1:645377/119‑1 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                                                >  2:645377/1‑119 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                                                >  4:1139556/1‑119 (MQ=255)
                                                                                            acCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTAc                                                                                <  3:1139556/119‑1 (MQ=255)
                                                                                              cccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                                                   <  1:744575/146‑1 (MQ=255)
                                                                                              cccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                                                   >  2:744575/1‑146 (MQ=255)
                                                                                               ccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                                               <  1:866498/53‑1 (MQ=255)
                                                                                               ccTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCctt                                                                                                                                               >  2:866498/1‑53 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                                                 <  4:193234/113‑1 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                                                 >  3:193234/1‑113 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGt                                                                            <  4:221094/118‑1 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGt                                                                            >  3:221094/1‑118 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgt                                                              <  4:477567/132‑1 (MQ=255)
                                                                                                 ttAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCCTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGgt                                                              >  3:477567/1‑132 (MQ=255)
                                                                                                  tAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACg                                                                                           <  3:302861/102‑1 (MQ=255)
                                                                                                  tAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACg                                                                                           >  4:302861/1‑102 (MQ=255)
                                                                                                    aaaCACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATg                                                                                                                                   <  1:565422/60‑1 (MQ=255)
                                                                                                    aaaCACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATg                                                                                                                                   >  2:565422/1‑60 (MQ=255)
                                                                                                      acacCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATa                                                                                             >  4:548600/1‑96 (MQ=255)
                                                                                                      acacCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATa                                                                                             <  3:548600/96‑1 (MQ=255)
                                                                                                         ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                                                   >  1:501192/1‑135 (MQ=255)
                                                                                                         ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTa                                                   <  2:501192/135‑1 (MQ=255)
                                                                                                         ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGaa                                         <  1:159539/145‑1 (MQ=255)
                                                                                                         ccAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGaa                                         >  2:159539/1‑145 (MQ=255)
                                                                                                                   gATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTg                                                                                                >  1:497332/1‑80 (MQ=255)
                                                                                                                   gATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTg                                                                                                <  2:497332/80‑1 (MQ=255)
                                                                                                                      aaaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTa                                                                                                                >  1:667499/1‑61 (MQ=255)
                                                                                                                      aaaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTa                                                                                                                <  2:667499/61‑1 (MQ=255)
                                                                                                                       aaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCa                                                                              <  1:597584/94‑1 (MQ=255)
                                                                                                                       aaaaaGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCa                                                                              >  2:597584/1‑94 (MQ=255)
                                                                                                                         gaaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGAc                      >  4:30718/2‑148 (MQ=255)
                                                                                                                          aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                                             >  1:484345/1‑124 (MQ=255)
                                                                                                                          aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                                             <  2:484345/124‑1 (MQ=255)
                                                                                                                          aaGGGCTGGCCAATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGa                                          <  2:124006/127‑1 (MQ=255)
                                                                                                                           agggctggccaATTGGCTGGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAg                    >  4:1033399/8‑148 (MQ=255)
                                                                                                                                 agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                                                 <  3:866964/81‑1 (MQ=255)
                                                                                                                                 agccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTa                                                                                 >  4:866964/1‑81 (MQ=255)
                                                                                                                                  gccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTg                        >  3:826203/1‑137 (MQ=255)
                                                                                                                                  gccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTg                        <  4:826203/137‑1 (MQ=255)
                                                                                                                                   ccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGATATCTTAGTTAAGACGCTCTTTGAt                                                                                              <  1:519878/66‑1 (MQ=255)
                                                                                                                                   ccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGt                                                      <  4:588333/106‑1 (MQ=255)
                                                                                                                                   ccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGt                                                      >  3:588333/1‑106 (MQ=255)
                                                                                                                                   ccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGt                           >  1:893488/1‑133 (MQ=255)
                                                                                                                                   ccaATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGt                           <  2:893488/133‑1 (MQ=255)
                                                                                                                                   ccaAATGGCCAGCCCTTCTTAACAGGATGGCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGAt                                                                                              >  2:519878/1‑66 (MQ=255)
                                                                                                                                      aTTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGt           <  2:526878/146‑1 (MQ=255)
                                                                                                                                      aTTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGt           >  1:526878/1‑146 (MQ=255)
                                                                                                                                        tGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGc                                                                  >  2:913686/1‑89 (MQ=255)
                                                                                                                                        tGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGc                                                                  <  1:913686/89‑1 (MQ=255)
                                                                                                                                             aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                                             <  3:882505/105‑1 (MQ=255)
                                                                                                                                             aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTa                                             >  4:882505/1‑105 (MQ=255)
                                                                                                                                             aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGtt                          >  2:1026540/1‑124 (MQ=255)
                                                                                                                                             aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGtt                          <  1:1026540/124‑1 (MQ=255)
                                                                                                                                             aGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCg  >  4:992071/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CCACACAGGGTTTAGGAAAAAAATATCCCATAGCCAGTAACAAGACCGCCCAGGGCCGCGCCGAGAACCGCCGCGTCGCAGTGGTGATTACTACCCCTTAAAACACCAGCAAACAGATAAAAAAGGGCCAGCCAATTGGCCAGCCCTTCTTAACAGGATGTCGCTTAAGCGAAATCTTAGTTAAGACGCTCTTTGATACGAGCAGCCTTACCAGTACGCTCACGCAGGTAGTACAGTTTAGCTTTACGAACAGCACCACGACGTTTGACAGAAATGCTGTCAACTACCG  >  NC_000913/2744007‑2744295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: