Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,761,623 C→A 12.4% V640L (GTA→TTA)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,761,6230CA12.4% 357.7 / 32.3 105V640L (GTA→TTA) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base C (45/47);  new base A (6/7);  total (51/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 111 total aligned reads displayed.

TCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCG  >  NC_000913/2761481‑2761761
                                                                                                                                              |                                                                                                                                          
tCCAGCAAGTCTACCAGTCGGTGTGACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       <  2:256499/148‑1 (MQ=255)
   aGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtggt                                                                                                                                    <  2:1041177/148‑1 (MQ=255)
    gCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       <  3:543671/144‑1 (MQ=255)
    gCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       >  4:543671/1‑144 (MQ=255)
             ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       <  3:1046095/135‑1 (MQ=255)
             ccAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       >  4:1046095/1‑135 (MQ=255)
                 tCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                                                          >  3:169877/1‑128 (MQ=255)
                 tCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAg                                                                                                                                          <  4:169877/128‑1 (MQ=255)
                         aCGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCaa                                                                                                              >  3:453716/1‑148 (MQ=255)
                           gTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc                                                                                                            <  4:453716/148‑1 (MQ=255)
                            tCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       >  2:878406/1‑120 (MQ=255)
                            tCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       <  1:878406/120‑1 (MQ=255)
                                   gggAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAcc                                                                                                    >  3:839117/1‑148 (MQ=255)
                                      aaTGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCt                                                                                                   >  3:612734/1‑146 (MQ=255)
                                            aaTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                           >  3:964532/1‑148 (MQ=255)
                                             aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       <  3:199910/103‑1 (MQ=255)
                                             aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       >  4:199910/1‑103 (MQ=255)
                                             aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                           <  3:2715/147‑1 (MQ=255)
                                             aTCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtagg                                                                                           >  4:2715/1‑147 (MQ=255)
                                              tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                                                                  <  2:1030786/107‑1 (MQ=255)
                                              tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGgtgt                                                                                                                                  >  1:1030786/1‑107 (MQ=255)
                                              tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt                                                                                              >  3:54270/1‑143 (MQ=255)
                                              tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt                                                                                              <  4:54270/143‑1 (MQ=255)
                                              tctcTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       <  3:972121/102‑1 (MQ=255)
                                               ctctTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 >  1:98786/1‑139 (MQ=255)
                                               ctctTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 <  2:98786/139‑1 (MQ=255)
                                                   tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTa                                                                                                                               <  1:1059337/105‑1 (MQ=255)
                                                   tctAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTa                                                                                                                               >  2:1059337/1‑105 (MQ=255)
                                                          cATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       <  2:47680/90‑1 (MQ=255)
                                                          cATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 >  4:33325/1‑128 (MQ=255)
                                                          cATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 <  3:33325/128‑1 (MQ=255)
                                                            ttGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                            >  3:629343/1‑83 (MQ=255)
                                                            ttGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                            <  4:629343/83‑1 (MQ=255)
                                                              gACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCTACTTTGCTGCGTATACCACGCTTTACAGCGTGCTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTg                                                                         <  4:839117/148‑1 (MQ=255)
                                                               acacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa                                                                                 <  4:165289/139‑1 (MQ=255)
                                                               acacCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGa                                                                                 >  3:165289/1‑139 (MQ=255)
                                                                  ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTa                                                                                                                           >  1:259516/1‑94 (MQ=255)
                                                                  ccTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTa                                                                     >  2:245960/1‑148 (MQ=255)
                                                                   cTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACCGCgtgg                                                                                                                                     <  2:549130/83‑1 (MQ=255)
                                                                   cTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtgg                                                                                                                                     >  1:549130/1‑83 (MQ=255)
                                                                    tggtggTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                                                          >  1:1107611/1‑141 (MQ=255)
                                                                    tggtggTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                                                          <  2:1107611/141‑1 (MQ=255)
                                                                            gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                                                                <  4:615018/127‑1 (MQ=255)
                                                                            gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGaa                                                                                >  3:615018/1‑127 (MQ=255)
                                                                            gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa                                                            <  4:570418/147‑1 (MQ=255)
                                                                            gATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCa                                                            >  3:570418/1‑147 (MQ=255)
                                                                              tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       >  2:723369/1‑70 (MQ=255)
                                                                              tGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTGTAAAGCgt                                                                                                                                       <  1:723369/70‑1 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       >  3:144959/1‑68 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTaa                                                                                                                              <  2:633019/77‑1 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTaa                                                                                                                              >  1:633019/1‑77 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAcc                                                                                                    <  4:223192/103‑1 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCAcc                                                                                                    >  3:223192/1‑103 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 >  1:87805/1‑106 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                                                          >  2:484473/1‑129 (MQ=255)
                                                                                gAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTAtt                                                                          <  1:484473/129‑1 (MQ=255)
                                                                                 aaaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag                                                                                            <  4:64542/110‑1 (MQ=255)
                                                                                  aaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag                                                                                            >  3:456341/1‑109 (MQ=255)
                                                                                  aaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggtag                                                                                            <  4:456341/109‑1 (MQ=255)
                                                                                  aaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt                                                                                              <  4:947727/107‑1 (MQ=255)
                                                                                  aaCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggt                                                                                              >  3:947727/1‑107 (MQ=255)
                                                                                   aCTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgt                                                                                                                                       >  4:185295/1‑65 (MQ=255)
                                                                                                aaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc                                                                                                            >  3:548422/1‑79 (MQ=255)
                                                                                                aaTTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGc                                                                                                            <  4:548422/79‑1 (MQ=255)
                                                                                                  tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                            >  1:115929/1‑45 (MQ=255)
                                                                                                  tttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTAc                                                                                                                                            <  2:115929/45‑1 (MQ=255)
                                                                                                   ttGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGaaa                                    <  1:245960/148‑1 (MQ=255)
                                                                                                       cATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtgg                                                                                                                                     <  3:219630/47‑1 (MQ=255)
                                                                                                       cATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCgtgg                                                                                                                                     >  4:219630/1‑47 (MQ=255)
                                                                                                            gtgtAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                             >  4:442785/1‑82 (MQ=255)
                                                                                                               tAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttt                                                                                                                                <  2:439097/44‑1 (MQ=255)
                                                                                                               tAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGttt                                                                                                                                >  1:439097/1‑44 (MQ=255)
                                                                                                                aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                             >  1:955263/1‑78 (MQ=255)
                                                                                                                aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGtat                                                                                       <  2:357254/84‑1 (MQ=255)
                                                                                                                aaTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGtat                                                                                       >  1:357254/1‑84 (MQ=255)
                                                                                                                aaTCGACTTTGCTGCGTATACCACGCTTTACAACGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTtaggta                                                                                             <  2:955263/78‑1 (MQ=255)
                                                                                                                 aTCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTcc                      <  4:964532/148‑1 (MQ=255)
                                                                                                                        ttGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGa                                                                                                         <  3:357326/58‑1 (MQ=255)
                                                                                                                        ttGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg                                                                               >  3:675317/1‑84 (MQ=255)
                                                                                                                        ttGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAg                                                                               <  4:675317/84‑1 (MQ=255)
                                                                                                                                 ataCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCtt                                                              >  1:418309/1‑92 (MQ=255)
                                                                                                                                 ataCCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCtt                                                              <  2:418309/92‑1 (MQ=255)
                                                                                                                                    ccACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCt                   <  4:900703/132‑1 (MQ=255)
                                                                                                                                    ccACGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCt                   >  3:900703/1‑132 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgttg                                                 <  3:216925/100‑1 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAttgttg                                                 >  4:216925/1‑100 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGa                                                <  1:17648/101‑1 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGa                                                >  2:17648/1‑101 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCtt                  >  3:360713/1‑131 (MQ=255)
                                                                                                                                      aCGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCtt                  <  4:360713/131‑1 (MQ=255)
                                                                                                                                      aCGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                           >  3:387356/1‑122 (MQ=255)
                                                                                                                                      aCGCTGTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                           <  4:387356/122‑1 (MQ=255)
                                                                                                                                       cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTg                                                                                                          <  4:685526/42‑1 (MQ=255)
                                                                                                                                       cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTg                                                                                                          >  3:685526/1‑42 (MQ=255)
                                                                                                                                       cGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCAt                                                      >  2:96423/1‑94 (MQ=255)
                                                                                                                                         cTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCg  >  1:54781/1‑144 (MQ=255)
                                                                                                                                         cTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCg  <  2:54781/144‑1 (MQ=255)
                                                                                                                                         cTTTAAAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTta                                                                                                 <  3:777668/49‑1 (MQ=255)
                                                                                                                                              cAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                           <  1:894906/114‑1 (MQ=255)
                                                                                                                                              cAGCGTGGAGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGtt                           >  2:894906/1‑114 (MQ=255)
                                                                                                                                              |                                                                                                                                          
TCCAGCAAGTCTACCAGTCGGTGTAACGTCTGAATGGGAATGCCAATCTCTTCTAAGGCATTGACACCTGGTGGTAGATGGAAACTTTCGAACGCCAATTTGACATGTGTGTAATCGACTTTGCTGCGTATACCACGCTTTACAGCGTGGTGTTTAACTACATCCTCCATCAAGCTGAGCACCTTAGGTAGGGTATAGCCGAAGGTATTGGTTATGAGCTTCAAATCATTGTTGAGGGCAATGGAAAGAGTACGTTTCTCCCCTTCAGAAATCCACTGTCG  >  NC_000913/2761481‑2761761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: