| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 285,882 | T→G | 27.7% | I163S (ATC→AGC) | yagG → | CP4‑6 prophage; putative sugar transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 285,882 | 0 | T | G | 27.7% | 81.1 / 41.7 | 47 | I163S (ATC→AGC) | yagG | CP4‑6 prophage; putative sugar transporter |
| Reads supporting (aligned to +/- strand): ref base T (17/17); new base G (6/7); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
GACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGT > NC_000913/285745‑286023 | gACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCAGCGCGATGCCg < 4:897245/148‑1 (MQ=255) cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcg > 1:227359/1‑138 (MQ=255) cccTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATcg < 2:227359/138‑1 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCgcgc < 4:1075341/126‑1 (MQ=255) tCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCgcgc > 3:1075341/1‑126 (MQ=255) catcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg < 1:354337/135‑1 (MQ=255) catcaCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTggg > 2:354337/1‑135 (MQ=255) aCCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgt > 3:926430/1‑148 (MQ=255) aaaGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGCGCATCATCGGCaaa > 2:170249/1‑102 (MQ=255) aaaGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaaa < 1:170249/102‑1 (MQ=255) cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑Gt < 4:188702/77‑1 (MQ=255) cACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑Gt > 3:188702/1‑77 (MQ=255) gCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTg > 1:1029415/1‑70 (MQ=255) gCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTg < 2:1029415/70‑1 (MQ=255) cAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGtgctgc < 4:926430/148‑1 (MQ=255) aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaa > 1:124626/1‑82 (MQ=255) aGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCaa < 2:124626/82‑1 (MQ=255) ggCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTg < 4:646381/92‑1 (MQ=255) ggCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTg > 3:646381/1‑92 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc > 4:344661/1‑90 (MQ=255) ttcttcCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGc < 3:344661/90‑1 (MQ=255) ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGcatca < 3:235788/56‑1 (MQ=255) ggcggcggcGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGcatca > 4:235788/1‑56 (MQ=255) ggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg > 4:682003/1‑93 (MQ=255) ggcggGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCAtgtg < 3:682003/93‑1 (MQ=255) cgctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTg > 1:332689/1‑94 (MQ=255) cgctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTg < 2:332689/94‑1 (MQ=255) gctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCt < 4:575220/148‑1 (MQ=255) gctATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCt > 3:575220/1‑148 (MQ=255) accAGCGGCAGCGCGATGCCGCTG‑‑‑ATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgcg > 4:1025917/3‑79 (MQ=37) accAGCGGCAGCGCGATGCCGCTG‑‑‑ATGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATgtgcg < 3:1025917/77‑1 (MQ=38) ccAGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTc < 4:193549/112‑1 (MQ=255) ccAGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTc > 3:193549/2‑113 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg < 2:744373/99‑1 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCgtggtg > 1:744373/1‑99 (MQ=255) aGCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAgg > 3:171459/1‑148 (MQ=255) gCGGCAGCGCGATGCCGCTGATAGCGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCt < 3:633498/88‑1 (MQ=25) gCGGCAGCGCGATGCCGCTGATAGCGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCt > 4:633498/1‑88 (MQ=25) gCGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGt < 4:171459/148‑1 (MQ=255) cGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCt < 4:154627/134‑1 (MQ=255) cGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCt > 3:154627/1‑134 (MQ=255) cGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaa > 3:412329/1‑126 (MQ=255) cGGCAGCGCGATGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCaa < 4:412329/126‑1 (MQ=255) cATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCg > 3:273177/1‑59 (MQ=255) cATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCg < 4:273177/59‑1 (MQ=255) aTCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACAcc > 1:789384/1‑135 (MQ=255) aTCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACCACCAAAGAGCGCTAcccc < 2:789384/135‑1 (MQ=255) | GACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCCATGCCGGGCGTCATCACCGCCGACCCGAAAGAGCGTCACGCCCTGCAGTCCTGGCGCTTCTTCCTGGCGGCGGCGGGCTCGCTCGCTATCAGCGGCATCGCGCTGCCGCTG‑‑‑GTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGT > NC_000913/285745‑286023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |