Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,866,487 G→C 44.4% intergenic (+22/+72) ygbN → / ← rpoS putative transporter/RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,4870GC44.4% 3.1 / 25.0 18intergenic (+22/+72)ygbN/rpoSputative transporter/RNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base C (4/4);  total (9/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

GGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGC  >  NC_000913/2866363‑2866601
                                                                                                                            |                                                                                                                  
ggggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCTTTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCgg                                                                                                             >  2:334435/1‑132 (MQ=255)
ggggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCgg                                                                                                             <  1:334435/132‑1 (MQ=255)
     tGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCAt                                                                                                                >  4:367068/1‑124 (MQ=255)
     tGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCAt                                                                                                                <  3:367068/124‑1 (MQ=255)
                             ccTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAc                                                                                                           >  4:98007/1‑105 (MQ=255)
                             ccTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAc                                                                                                           <  3:98007/105‑1 (MQ=255)
                                                                       tAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg                                                                                                                   >  3:895643/1‑55 (MQ=255)
                                                                       tAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg                                                                                                                   <  4:895643/55‑1 (MQ=255)
                                                                                            cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCtt                                                                         >  1:123969/1‑76 (MQ=255)
                                                                                            cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCtt                                                                         <  2:123969/76‑1 (MQ=255)
                                                                                                       ccTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctta                                              <  3:114224/92‑1 (MQ=255)
                                                                                                       ccTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctta                                              >  4:114224/1‑92 (MQ=255)
                                                                                                                  aGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                   <  3:1110397/60‑1 (MQ=37)
                                                                                                                  aGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt                                                                   >  4:1110397/1‑60 (MQ=37)
                                                                                                                    gTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGc  <  2:334316/123‑1 (MQ=255)
                                                                                                                    gTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGc  >  1:334316/1‑123 (MQ=255)
                                                                                                                     tCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTgg                                                                 >  3:5061/1‑59 (MQ=38)
                                                                                                                     tCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTgg                                                                 <  4:5061/59‑1 (MQ=38)
                                                                                                                         aTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCa                >  3:896528/1‑104 (MQ=255)
                                                                                                                         aTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCa                <  4:896528/104‑1 (MQ=255)
                                                                                                                            |                                                                                                                  
GGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGC  >  NC_000913/2866363‑2866601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: