| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,866,488 | G→C | 44.4% | intergenic (+23/+71) | ygbN → / ← rpoS | putative transporter/RNA polymerase, sigma S (sigma 38) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,866,488 | 0 | G | C | 44.4% | 2.9 / 25.1 | 18 | intergenic (+23/+71) | ygbN/rpoS | putative transporter/RNA polymerase, sigma S (sigma 38) factor |
| Reads supporting (aligned to +/- strand): ref base G (5/5); new base C (4/4); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 | |||||||||||
GGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGC > NC_000913/2866363‑2866601 | ggggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCTTTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCgg > 2:334435/1‑132 (MQ=255)ggggTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGCCCATCgg < 1:334435/132‑1 (MQ=255) tGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCAt > 4:367068/1‑124 (MQ=255) tGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCAt < 3:367068/124‑1 (MQ=255) ccTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAc > 4:98007/1‑105 (MQ=255) ccTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGAc < 3:98007/105‑1 (MQ=255) tAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg > 3:895643/1‑55 (MQ=255) tAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATggg < 4:895643/55‑1 (MQ=255) cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCtt > 1:123969/1‑76 (MQ=255) cGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCtt < 2:123969/76‑1 (MQ=255) ccTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctta < 3:114224/92‑1 (MQ=255) ccTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGctta > 4:114224/1‑92 (MQ=255) aGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt < 3:1110397/60‑1 (MQ=37) aGGTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACt > 4:1110397/1‑60 (MQ=37) gTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGc < 2:334316/123‑1 (MQ=255) gTCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGc > 1:334316/1‑123 (MQ=255) tCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTgg > 3:5061/1‑59 (MQ=38) tCCGATGCCCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTgg < 4:5061/59‑1 (MQ=38) aTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCa > 3:896528/1‑104 (MQ=255) aTGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCa < 4:896528/104‑1 (MQ=255) | GGGGTTGTCGGTAGCAGACGGTCTGAAAACCTGGACAGTGTTAACGACCATTCTCGGTTTTACCGGATTCTTAATTACCTGGTGCGTATGGGCGGTAATTTGACCTTAATAAAAAGGTCCGATGGGCATCGGACCTTTTATTGTGCACAGAAAAGGCCAGCCTCGCTTGAGACTGGCCTTTCTGACAGATGCTTACTTACTCGCGGAACAGCGCTTCGATATTCAGCCCCTGCGTTTGC > NC_000913/2866363‑2866601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |