| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,868,883 | T→C | 5.5% | intergenic (‑130/+10) | nlpD ← / ← pcm | activator of AmiC murein hydrolase activity, lipoprotein/L‑isoaspartate protein carboxylmethyltransferase type II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,868,883 | 0 | T | C | 5.5% | 251.4 / 3.5 | 73 | intergenic (‑130/+10) | nlpD/pcm | activator of AmiC murein hydrolase activity, lipoprotein/L‑isoaspartate protein carboxylmethyltransferase type II |
| Reads supporting (aligned to +/- strand): ref base T (34/35); new base C (2/2); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01 | |||||||||||
CATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGA > NC_000913/2868751‑2869014 | cATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGc < 1:594098/148‑1 (MQ=255) ccccAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAg < 1:106613/139‑1 (MQ=255) ccccAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAg < 2:67825/139‑1 (MQ=255) ccccAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAg > 2:106613/1‑139 (MQ=255) ccccAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAg > 1:67825/1‑139 (MQ=255) aaaTTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTa > 2:230180/1‑128 (MQ=255) aaaTTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTa < 1:230180/128‑1 (MQ=255) aagtGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGcc > 4:96755/4‑131 (MQ=255) aaTTGGTTAATAACCAGTGACATAATAACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGcc < 3:96755/131‑1 (MQ=255) taataaCCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa < 1:403991/142‑1 (MQ=255) taataaCCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa > 2:403991/1‑142 (MQ=255) taataaCCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa < 3:393004/142‑1 (MQ=255) taataaCCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa > 4:393004/1‑142 (MQ=255) cATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCAc < 3:580164/125‑1 (MQ=255) cATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCAc > 4:580164/1‑125 (MQ=255) cATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACaa > 2:481844/1‑136 (MQ=255) cATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACaa < 1:481844/136‑1 (MQ=255) ttACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAg > 2:841820/1‑108 (MQ=255) ttACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAg < 1:841820/108‑1 (MQ=255) tACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTCCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAggg > 2:11982/1‑126 (MQ=255) tACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTCCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAggg < 1:11982/126‑1 (MQ=255) tACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTCCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAggg > 2:282079/1‑126 (MQ=255) tACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTCCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAggg < 1:282079/126‑1 (MQ=255) aCCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa < 1:543732/122‑1 (MQ=255) aCCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTaaa > 2:543732/1‑122 (MQ=255) ggCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCAc < 1:42396/109‑1 (MQ=255) ggCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCAc > 2:42396/1‑109 (MQ=255) ggCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGata > 3:689497/1‑146 (MQ=255) ggCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGata < 4:689497/146‑1 (MQ=255) ccTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTc < 3:203229/102‑1 (MQ=255) ccTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTc > 4:203229/1‑102 (MQ=255) aCTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAAtt < 4:397762/145‑1 (MQ=255) aCTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAAtt > 3:397762/1‑145 (MQ=255) aaCACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGa > 3:179880/1‑132 (MQ=255) aaCACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGa < 4:179880/132‑1 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCGAGGTATTTCCTCACGTTTTAAGCCAg < 3:444130/79‑1 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCGAGGTATTTCCTCACGTTTTAAGCCAg > 4:444130/1‑79 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAgcgc < 2:209813/107‑1 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAgcgc > 1:209813/1‑107 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGc < 4:1086432/112‑1 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGc > 3:1086432/1‑112 (MQ=255) ggAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCcgacg > 4:788424/1‑148 (MQ=255) aaaaGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAAt > 2:644794/1‑132 (MQ=255) aaaaGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAAt < 1:644794/132‑1 (MQ=255) tGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTc < 4:317500/81‑1 (MQ=255) tGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTc > 3:317500/1‑81 (MQ=255) tCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTcc > 2:215568/1‑105 (MQ=255) tCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTcc < 1:215568/105‑1 (MQ=255) aCGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTa > 1:1081281/1‑109 (MQ=255) aCGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTa < 2:1081281/109‑1 (MQ=255) gATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACaa > 3:867298/1‑87 (MQ=255) gATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACaa < 4:867298/87‑1 (MQ=255) aTACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCg > 3:892831/1‑121 (MQ=255) aTACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCg < 4:892831/121‑1 (MQ=255) tGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTgg < 3:788424/148‑1 (MQ=255) tGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCt < 3:1032806/37‑1 (MQ=255) tGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCt > 4:1032806/1‑37 (MQ=255) aTAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTTAAGGGACAAAGCGCACGGCCTCCACGGTATCGAtaataa < 1:230664/96‑1 (MQ=255) aTAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGAtaataa > 2:230664/1‑96 (MQ=255) aGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAgcgc > 3:568212/1‑65 (MQ=255) aGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAgcgc < 4:568212/65‑1 (MQ=255) aaaaaTCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTAc > 3:326414/1‑139 (MQ=255) aaaaaTCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTAc < 4:326414/139‑1 (MQ=255) ccAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCg > 1:880632/1‑86 (MQ=255) ccAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCg < 2:880632/86‑1 (MQ=255) aGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGct > 2:413323/1‑121 (MQ=255) aGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGct < 1:413323/121‑1 (MQ=255) ggTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTcccc > 2:721786/1‑127 (MQ=255) ggTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTcccc < 1:721786/127‑1 (MQ=255) ggTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACggg > 2:563822/1‑133 (MQ=255) ggTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACggg < 1:563822/133‑1 (MQ=255) tATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGa < 4:1128636/136‑1 (MQ=255) tATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGa > 3:1128636/1‑136 (MQ=255) | CATTTATCCCCCAGGAAAAATTGGTTAATAACCAGTGACATAATTACCGTGCAAGGCACCCTACTGAACACTGGAAAAGATGTTCACGATACGCTGACCTGCGGCAAAATAACCAGGAAAAATCCAGGTATTTCCTCACGTTTTAAGCCAGCTCACCCTTCACTAAAGGGACAAAGCGCACGGCCTCCACGGTATCGATAATAAATTCGCCTCCCCGACGACGCACCCGTTTCAAATACTGGTGCTCCTCCCCTACGGGTAAGA > NC_000913/2868751‑2869014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |