Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,960,928 C→G 6.0% G501R (GGC→CGC) ‡ recC ← exonuclease V (RecBCD complex), gamma chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,960,9280CG 6.0% 535.7 / 16.7 147G501R (GGC→CGC) ‡recCexonuclease V (RecBCD complex), gamma chain
Reads supporting (aligned to +/- strand):  ref base C (68/70);  new base G (5/4);  total (73/74)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

AAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTT  >  NC_000913/2960781‑2961074
                                                                                                                                                   |                                                                                                                                                  
aaGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGcc                                                                                                                                                    >  1:404252/1‑148 (MQ=255)
          cGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          <  3:823084/148‑1 (MQ=255)
                  aCTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCGCTg                                                                                                                                  <  2:214256/148‑1 (MQ=255)
                  aCTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTg                                                                                                                                  >  1:485378/1‑148 (MQ=255)
                   cTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTgg                                                                                                                                 >  3:103631/1‑148 (MQ=255)
                        ccTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTaa                                                                                                                            >  4:131381/1‑148 (MQ=255)
                               gcTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGc                                                                                                                     <  2:336837/148‑1 (MQ=255)
                               gcTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGc                                                                                                                     <  2:485378/148‑1 (MQ=255)
                                   tcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa                                                                                                                                     <  2:341625/128‑1 (MQ=255)
                                   tcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa                                                                                                                                     >  1:341625/1‑128 (MQ=255)
                                    ccATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGa                                                                                                                                         <  2:745765/123‑1 (MQ=255)
                                      aTCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          <  4:654734/120‑1 (MQ=255)
                                      aTCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          >  3:654734/1‑120 (MQ=255)
                                      aTCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATaa                                                                                                                        >  2:876159/1‑138 (MQ=255)
                                      aTCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATaa                                                                                                                        <  1:876159/138‑1 (MQ=255)
                                      aTCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATa                                                                                                                         >  1:830941/1‑137 (MQ=255)
                                       tCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          >  4:1021347/1‑119 (MQ=255)
                                         gcgTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCcttctt                                                                                                           <  2:404252/148‑1 (MQ=255)
                                          cgTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCttcttc                                                                                                          <  4:171448/148‑1 (MQ=255)
                                           gTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCg                                                                                                         <  1:40484/148‑1 (MQ=255)
                                             aGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGAAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCctt                                                                                                              <  1:784074/141‑1 (MQ=255)
                                             aGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGAAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCctt                                                                                                              >  2:784074/1‑141 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGTTTCGTTGACCCAc                                                                                                                                    >  1:588868/1‑117 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGTTTCGTTGACCCAc                                                                                                                                    <  2:588868/117‑1 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          <  3:204557/111‑1 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          >  4:204557/1‑111 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa                                                                                                     <  4:103631/148‑1 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa                                                                                                     >  4:757725/1‑148 (MQ=255)
                                               cccAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa                                                                                                     <  2:1093025/148‑1 (MQ=255)
                                                 caacaaCATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATg                                                                                                   >  2:267910/1‑148 (MQ=255)
                                                  aacaacATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          <  3:1003388/108‑1 (MQ=255)
                                                  aacaacATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          >  4:1003388/1‑108 (MQ=255)
                                                  aacaacATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGt                                                                                                  <  3:131381/148‑1 (MQ=255)
                                                    caacaTACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCgg                                                                                                        <  2:96064/140‑1 (MQ=255)
                                                     aacaTACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCaa                                                                                               >  4:918791/1‑148 (MQ=255)
                                                          aCGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGa                                                                                                     <  3:556223/137‑1 (MQ=255)
                                                               tCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCttcttc                                                                                                          <  4:662628/127‑1 (MQ=255)
                                                                    ccAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg                                                                                                                                          <  1:356354/90‑1 (MQ=255)
                                                                     cAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGCGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCAc                                                                                                                                    >  2:1065588/1‑95 (MQ=255)
                                                                     cAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCAc                                                                                                                                    <  1:1065588/95‑1 (MQ=255)
                                                                     cAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCttcttc                                                                                                          >  3:988217/1‑121 (MQ=255)
                                                                       aaTCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCg                                                                             <  3:918791/148‑1 (MQ=255)
                                                                        aTCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCaa                                                                                               >  1:713969/1‑129 (MQ=255)
                                                                             cAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATCCGGCATTCGtt                                                                                                                                           >  2:1004428/1‑80 (MQ=255)
                                                                             cAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATCCGGCATTCGtt                                                                                                                                           <  1:1004428/80‑1 (MQ=255)
                                                                              aGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATc                                                                      <  3:967811/148‑1 (MQ=255)
                                                                                    gttgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCgg                                                                                                        <  3:205100/108‑1 (MQ=255)
                                                                                      tgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGcc                                                                                                                 <  2:38366/97‑1 (MQ=255)
                                                                                      tgtCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCaa                                                                                               >  4:309631/1‑115 (MQ=255)
                                                                                        tCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTg                                                                                                      <  4:328724/106‑1 (MQ=255)
                                                                                              ggCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCg                                                                                                                                             >  2:843701/1‑61 (MQ=255)
                                                                                                cGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATc                                                    >  4:318095/1‑148 (MQ=255)
                                                                                                    gAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATc                                                                      >  2:527505/1‑126 (MQ=255)
                                                                                                    gAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATc                                                                      <  1:527505/126‑1 (MQ=255)
                                                                                                          cAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGAcaca                                          >  1:283113/1‑148 (MQ=255)
                                                                                                           aGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGc                                                                                                                  >  3:1009778/1‑75 (MQ=255)
                                                                                                            gCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGcgccg                                                                                      <  1:993803/102‑1 (MQ=255)
                                                                                                            gCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGcgccg                                                                                      >  2:993803/1‑102 (MQ=255)
                                                                                                                 cgAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGa                                    <  2:455265/147‑1 (MQ=255)
                                                                                                                 cgAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGa                                    >  1:455265/1‑147 (MQ=255)
                                                                                                                     cGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTc                               <  3:318095/148‑1 (MQ=255)
                                                                                                                       ttGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCct                                                  >  4:1075330/1‑127 (MQ=255)
                                                                                                                       ttGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTg                                               <  2:292908/130‑1 (MQ=255)
                                                                                                                       ttGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTg                                               >  1:292908/1‑130 (MQ=255)
                                                                                                                       ttGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTg                                               <  1:931803/130‑1 (MQ=255)
                                                                                                                       ttGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAg                             <  3:773426/148‑1 (MQ=255)
                                                                                                                             tcATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTg                                                                                                      >  3:1046132/1‑69 (MQ=255)
                                                                                                                             tcATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTg                                                                                                      <  4:806535/69‑1 (MQ=255)
                                                                                                                             tcATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTg                       >  2:420416/1‑148 (MQ=255)
                                                                                                                              cATCTATGCCCCAACGAATGGCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGa                      >  2:279309/1‑148 (MQ=255)
                                                                                                                              cATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGac                                             <  1:1082990/125‑1 (MQ=255)
                                                                                                                                tCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCaa                                                                                               <  1:259074/73‑1 (MQ=255)
                                                                                                                                tCTAAGCCCCAACGAATGCCGGATTCGTTGACTCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATcgt                                                  >  1:320995/1‑116 (MQ=255)
                                                                                                                                 cTATGCCCCAACGAATGGCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATaa                   <  1:279309/148‑1 (MQ=255)
                                                                                                                                   aTGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACcg                                                                                           >  3:665233/1‑74 (MQ=255)
                                                                                                                                   aTGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCctc                                                 >  1:256328/1‑116 (MQ=255)
                                                                                                                                   aTGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCctc                                                 <  2:256328/116‑1 (MQ=255)
                                                                                                                                   aTGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGAc                                   >  3:799703/1‑130 (MQ=255)
                                                                                                                                   aTGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGAc                                   <  4:799703/130‑1 (MQ=255)
                                                                                                                                    tGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACa                 <  3:319483/147‑1 (MQ=255)
                                                                                                                                    tGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAg                <  2:1065771/148‑1 (MQ=255)
                                                                                                                                    tGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAg                >  3:378434/1‑148 (MQ=255)
                                                                                                                                    tGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAg                >  3:1086035/1‑148 (MQ=255)
                                                                                                                                     gCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAgg               <  4:1086035/148‑1 (MQ=255)
                                                                                                                                         cAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATg                                                                                                   >  2:467306/1‑60 (MQ=255)
                                                                                                                                         cAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATg                                                                                                   <  1:467306/60‑1 (MQ=255)
                                                                                                                                          aaCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGTCACAAAACGACTGt                                <  4:845221/126‑1 (MQ=255)
                                                                                                                                          aaCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGt                                >  3:845221/1‑126 (MQ=255)
                                                                                                                                          aaCGAATCCGGCATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAgg               <  2:524707/143‑1 (MQ=255)
                                                                                                                                          aaCGAATCCGGCATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAgg               >  1:524707/1‑143 (MQ=255)
                                                                                                                                           aCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCa                                                                                <  2:290580/77‑1 (MQ=255)
                                                                                                                                           aCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCgg                                                                            <  4:727482/81‑1 (MQ=255)
                                                                                                                                           aCGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCgg                                                                            >  3:727482/1‑81 (MQ=255)
                                                                                                                                           aCGAATCCGGCATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCa                                                                    <  1:117034/89‑1 (MQ=255)
                                                                                                                                           aCGAATCCGGCATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATa                    >  4:455670/1‑137 (MQ=255)
                                                                                                                                           aCGAATCCGGCATTCGTTGACCCACTGGCGTAAATAAAGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCa                                                                    >  2:117034/1‑89 (MQ=255)
                                                                                                                                              aaTGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGt                        >  4:980818/1‑130 (MQ=255)
                                                                                                                                               aTGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCa                                                           >  1:145878/1‑94 (MQ=255)
                                                                                                                                               aTGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCa                                                           <  2:145878/94‑1 (MQ=255)
                                                                                                                                                  ccGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCtt  <  1:18630/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCACCGGCACATCCAGCAACGCCAGCACATCCTCTGACACAAAACGACTGTCAGGCAGTGATAACAGGCTGATAAACGCTT  >  NC_000913/2960781‑2961074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: