Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,742 A→G 23.1% intergenic (‑107/+22) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7420AG23.1% 47.9 / 12.7 26intergenic (‑107/+22)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (10/10);  new base G (3/3);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

CAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGT  >  NC_000913/2980601‑2980859
                                                                                                                                             |                                                                                                                     
cAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcac                                                                                                                 >  2:684597/1‑148 (MQ=255)
                        aTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCg                                                                                                            <  1:635174/129‑1 (MQ=255)
                        aTTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCg                                                                                                            >  2:635174/1‑129 (MQ=255)
                                   tCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTa                                                                                                                       >  1:514314/1‑107 (MQ=255)
                                   tCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTa                                                                                                                       <  2:514314/107‑1 (MQ=255)
                                      tctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                                                <  4:109223/111‑1 (MQ=255)
                                      tctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                                                >  3:109223/1‑111 (MQ=255)
                                          tcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtctc                                                                       <  1:684597/148‑1 (MQ=255)
                                                                   gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTAc                                                                                                                      <  1:998310/76‑1 (MQ=255)
                                                                   gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTAc                                                                                                                      >  2:998310/1‑76 (MQ=255)
                                                                        aTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATc                                                                                                              <  4:896545/79‑1 (MQ=255)
                                                                        aTAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATc                                                                                                              >  3:896545/1‑79 (MQ=255)
                                                                         tAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCg                                                                                                            >  4:884990/1‑80 (MQ=38)
                                                                         tAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCg                                                                                                            <  3:884990/80‑1 (MQ=38)
                                                                             gAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCg                                    >  3:917259/1‑148 (MQ=255)
                                                                                 gagaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTAc                                                                                                                      <  1:862935/62‑1 (MQ=255)
                                                                                 gagaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTAc                                                                                                                      >  2:862935/1‑62 (MQ=255)
                                                                                                           cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCg                                                 >  4:463201/1‑105 (MQ=255)
                                                                                                           cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCg                                                 <  3:463201/105‑1 (MQ=255)
                                                                                                               aTCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGt  <  4:917259/148‑1 (MQ=255)
                                                                                                                                 aGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                               <  3:440724/69‑1 (MQ=255)
                                                                                                                                 aGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                               >  2:993504/1‑69 (MQ=255)
                                                                                                                                 aGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                               >  4:440724/1‑69 (MQ=255)
                                                                                                                                 aGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                                               <  1:993504/69‑1 (MQ=255)
                                                                                                                                      cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg   >  2:360593/1‑124 (MQ=255)
                                                                                                                                      cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAg   <  1:360593/124‑1 (MQ=255)
                                                                                                                                       ggATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa               >  2:948437/1‑111 (MQ=255)
                                                                                                                                       ggATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGaa               <  1:948437/111‑1 (MQ=255)
                                                                                                                                             |                                                                                                                     
CAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGT  >  NC_000913/2980601‑2980859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: