| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,993,279 | G→T | 5.9% | D396Y (GAC→TAC) | ygeH → | putative transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,993,279 | 0 | G | T | 5.9% | 242.8 / 5.4 | 68 | D396Y (GAC→TAC) | ygeH | putative transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (32/32); new base T (2/2); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
ACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAG > NC_000913/2993149‑2993414 | aCAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTc < 4:418882/148‑1 (MQ=255) cAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAg > 2:359733/1‑126 (MQ=255) cAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAg < 1:359733/126‑1 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa < 4:865931/116‑1 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa < 2:314258/116‑1 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa > 1:314258/1‑116 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa > 3:865931/1‑116 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCAc > 2:808645/1‑117 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCAc < 1:808645/117‑1 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACt > 2:906806/1‑118 (MQ=255) tATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACt < 1:906806/118‑1 (MQ=255) ttGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTCTATACAAATc > 3:695867/1‑112 (MQ=255) ttGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTCTATACAAATc < 4:695867/112‑1 (MQ=255) aaCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACt > 4:816929/1‑118 (MQ=255) aaCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACt < 3:816929/118‑1 (MQ=255) aaCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGAt > 1:519014/1‑148 (MQ=255) aCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGa < 4:163430/102‑1 (MQ=255) aCAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGa > 3:163430/1‑102 (MQ=255) aaGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa > 2:801830/1‑102 (MQ=255) aaGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa < 2:499590/102‑1 (MQ=255) aaGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa > 1:499590/1‑102 (MQ=255) aaGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCa < 1:801830/102‑1 (MQ=255) cTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACa < 4:155322/99‑1 (MQ=255) cTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACa > 3:155322/1‑99 (MQ=255) tCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGata < 2:519014/148‑1 (MQ=255) aaCTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCaga > 2:1083793/1‑109 (MQ=255) aaCTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCaga < 1:1083793/109‑1 (MQ=255) tctctctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATaac < 2:1079174/119‑2 (MQ=255) tctctctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATaaa > 1:1079174/1‑119 (MQ=255) tctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa < 4:1102552/118‑1 (MQ=255) tctACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGa > 3:1102552/1‑118 (MQ=255) actactaTAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGaa > 2:1003483/1‑83 (MQ=255) actactaTAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGaa < 1:1003483/83‑1 (MQ=255) actactaTAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCaa > 4:411816/1‑148 (MQ=255) ctactaTAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCaaa < 3:411816/148‑1 (MQ=255) gCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAtgt < 3:57647/108‑1 (MQ=255) gCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAtgt > 4:57647/1‑108 (MQ=255) aCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAg < 2:897309/110‑1 (MQ=255) aCTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAg > 1:897309/1‑110 (MQ=255) cTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGa > 2:950344/1‑94 (MQ=255) cTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGa < 1:950344/94‑1 (MQ=255) ccACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGc > 4:433390/1‑81 (MQ=255) ccACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGc < 3:433390/81‑1 (MQ=255) ccACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAt < 1:648260/98‑1 (MQ=255) ccACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAAt > 2:648260/1‑98 (MQ=255) aaTGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGata > 2:1121758/1‑97 (MQ=255) aaTGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGata < 1:1121758/97‑1 (MQ=255) aTTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTaa > 2:569317/1‑122 (MQ=255) aTTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCGAACCCGCTCAAAAACAACATTaa < 1:569317/122‑1 (MQ=255) ttGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTtgtg < 4:499004/68‑1 (MQ=255) ttGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTtgtg > 3:499004/1‑68 (MQ=255) agGCGAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGaa < 3:613443/131‑1 (MQ=255) agGCAAGGATTTGTATAGACAAATCACTACAACTCGa > 1:807479/1‑37 (MQ=255) agGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGaa > 4:613443/1‑131 (MQ=255) agGCAAGGAATTGTATAGACAAATCACTACAACTCGa < 2:807479/37‑1 (MQ=255) cAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTcta > 2:531155/1‑118 (MQ=255) cAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTcta < 1:531155/118‑1 (MQ=255) aGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTAtgtg > 1:616594/1‑84 (MQ=255) aGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAg > 4:717666/1‑148 (MQ=255) aGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATCTAtgtg < 2:616594/84‑1 (MQ=255) gATTTGTCTATACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGa < 3:630444/145‑1 (MQ=255) gATTTGTCTATACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGa > 4:630444/1‑145 (MQ=255) ttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAAcc < 2:1004734/86‑1 (MQ=255) ttGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAAcc > 1:1004734/1‑86 (MQ=255) ataGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgt > 3:829747/1‑70 (MQ=255) ataGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGAtatgt < 4:829747/70‑1 (MQ=255) aGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAaaca > 4:104733/1‑92 (MQ=255) aGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAaaca < 3:104733/92‑1 (MQ=255) aGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTga > 2:705513/1‑120 (MQ=255) aGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTga < 1:705513/120‑1 (MQ=255) | ACAAGCAAAAGTATCTCATATCTTATTTGAACAGGCTAAGATACACTCAACTGATATAGCCTCTCTCTACTACTATAGGGCACTAGTCCACTTTCATAATGAAAAAATTGAAGAGGCAAGGATTTGTATAGACAAATCACTACAACTCGAACCCAGAAGACGAAAAGCAGTTGTGATAAAAGAATGTGTAGATATGTATGTGCCTAACCCGCTCAAAAACAACATTAAACTCTACTATAAAGAAACTGAGAGTGAAAGCCATCGAG > NC_000913/2993149‑2993414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |