Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,033,627 T→A 11.5% intergenic (+14/+30) idi → / ← lysS isopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6270TA11.5% 191.3 / 14.6 61intergenic (+14/+30)idi/lysSisopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base A (3/4);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

TGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAG  >  NC_000913/3033488‑3033771
                                                                                                                                           |                                                                                                                                                
tgtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCTAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCggg                                                                                                                                          <  1:789630/148‑1 (MQ=255)
 gtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCgggg                                                                                                                                         >  2:43728/1‑148 (MQ=255)
            gATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGcc                                                                                                                                             <  1:258290/133‑1 (MQ=255)
            gATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGcc                                                                                                                                             >  2:258290/1‑133 (MQ=255)
                    acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATg                                                                                                                      <  1:43728/148‑1 (MQ=255)
                       cGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGc                                                                                                                                 <  1:129697/134‑1 (MQ=255)
                       cGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGc                                                                                                                                 >  2:129697/1‑134 (MQ=255)
                                    aCGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACg                                                                                                      >  2:402625/1‑148 (MQ=255)
                                     cGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                               <  1:1120857/106‑1 (MQ=255)
                                     cGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                               >  2:1120857/1‑106 (MQ=255)
                                        cGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATc                                                                                                  >  3:367198/1‑148 (MQ=255)
                                                 tCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAAc                                                                                         <  4:367198/148‑1 (MQ=255)
                                                      ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGa                                                                                      >  4:804501/1‑146 (MQ=255)
                                                      ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGa                                                                                      <  3:804501/146‑1 (MQ=255)
                                                                       ggCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt                                                                   >  4:248323/1‑148 (MQ=255)
                                                                           aCAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg                                                                  <  1:405136/145‑1 (MQ=255)
                                                                           aCAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg                                                                  >  2:405136/1‑145 (MQ=255)
                                                                                 cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                   >  3:1006021/1‑106 (MQ=255)
                                                                                 cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                   <  4:1006021/106‑1 (MQ=255)
                                                                                 cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            <  1:785053/145‑1 (MQ=255)
                                                                                 cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            >  2:785053/1‑145 (MQ=255)
                                                                                           gAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCtt                                                                                                                   <  2:876679/80‑1 (MQ=255)
                                                                                           gAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCtt                                                                                                                   >  1:876679/1‑80 (MQ=255)
                                                                                            aaaaCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa                                              <  1:402625/148‑1 (MQ=255)
                                                                                                  aTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                        >  2:1045027/1‑52 (MQ=255)
                                                                                                  aTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                        <  1:1045027/52‑1 (MQ=255)
                                                                                                  aTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                     <  2:254481/71‑1 (MQ=255)
                                                                                                  aTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                     >  1:254481/1‑71 (MQ=255)
                                                                                                        tGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            >  4:312956/1‑122 (MQ=255)
                                                                                                        tGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            <  3:312956/122‑1 (MQ=255)
                                                                                                                  cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTg                                                              <  4:69437/110‑1 (MQ=255)
                                                                                                                  cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTg                                                              >  3:69437/1‑110 (MQ=255)
                                                                                                                       taaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                         >  4:621359/1‑110 (MQ=255)
                                                                                                                       taaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                         <  3:621359/110‑1 (MQ=255)
                                                                                                                            aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATATCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                             <  1:394131/117‑1 (MQ=255)
                                                                                                                            aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                             >  2:394131/1‑117 (MQ=255)
                                                                                                                             aaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa                                                                                  >  3:676637/1‑79 (MQ=255)
                                                                                                                             aaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa                                                                                  <  4:676637/79‑1 (MQ=255)
                                                                                                                              aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                     <  1:423775/107‑1 (MQ=255)
                                                                                                                              aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                     >  2:423775/1‑107 (MQ=255)
                                                                                                                              aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                             <  3:21192/115‑1 (MQ=255)
                                                                                                                              aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                             >  4:21192/1‑115 (MQ=255)
                                                                                                                                caaCCCCGGCAAATGTCGGGGTTTTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                        <  3:63656/133‑1 (MQ=255)
                                                                                                                                caaCCCCGGCAAATGTCGGGGTTTTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                        >  4:63656/2‑134 (MQ=255)
                                                                                                                                aaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg          >  4:189838/1‑148 (MQ=255)
                                                                                                                                  aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                        <  4:544105/132‑1 (MQ=255)
                                                                                                                                  aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                        >  3:544105/1‑132 (MQ=255)
                                                                                                                                   ccccGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                         >  2:230621/1‑98 (MQ=255)
                                                                                                                                   ccccGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                         <  1:230621/98‑1 (MQ=255)
                                                                                                                                   ccccGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                 <  3:450167/122‑1 (MQ=255)
                                                                                                                                   ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcg       >  3:237569/1‑148 (MQ=255)
                                                                                                                                     ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt     >  4:511062/1‑148 (MQ=255)
                                                                                                                                       gACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            <  4:445980/91‑1 (MQ=255)
                                                                                                                                       gACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                            >  3:445980/1‑91 (MQ=255)
                                                                                                                                        aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                 >  4:830942/1‑85 (MQ=255)
                                                                                                                                        aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                 <  3:830942/85‑1 (MQ=255)
                                                                                                                                        aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                          <  3:680359/92‑1 (MQ=255)
                                                                                                                                        aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                          >  4:680359/1‑92 (MQ=255)
                                                                                                                                        aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAg  <  3:189838/148‑1 (MQ=255)
                                                                                                                                         cATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                          <  2:847741/91‑1 (MQ=255)
                                                                                                                                         cATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                          >  1:847741/1‑91 (MQ=255)
                                                                                                                                           |                                                                                                                                                
TGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAG  >  NC_000913/3033488‑3033771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑