Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,140 A→G 8.7% intergenic (+152/+38) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1400AG 8.7% 293.0 / 14.9 92intergenic (+152/+38)sibC/serAsRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (41/43);  new base G (4/4);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-01

CCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAA  >  NC_000913/3057001‑3057280
                                                                                                                                           |                                                                                                                                            
ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                      <  1:453887/148‑1 (MQ=255)
          ctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                                                            >  4:490922/1‑148 (MQ=255)
               aTCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                                                       >  4:700/1‑148 (MQ=255)
                 cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                                                     <  3:18176/148‑1 (MQ=255)
                 cGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTc                                                                                                                     <  3:490922/148‑1 (MQ=255)
                         cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgg                                                                                                             >  3:501897/1‑148 (MQ=255)
                             ttAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                                                         <  4:330033/132‑1 (MQ=255)
                             ttAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                                                         >  3:330033/1‑132 (MQ=255)
                              taaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTc                                                                                                                                           >  3:574905/1‑113 (MQ=255)
                              taaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTc                                                                                                                                           <  4:574905/113‑1 (MQ=255)
                                 ataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                       <  2:976160/114‑1 (MQ=255)
                                 ataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                       >  1:976160/1‑114 (MQ=255)
                                   aaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                                                                  >  4:539398/1‑117 (MQ=255)
                                   aaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                                                                  <  3:539398/117‑1 (MQ=255)
                                    aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                  <  3:700/148‑1 (MQ=255)
                                          aCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                                                 <  1:222710/143‑1 (MQ=255)
                                          aCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAca                                                                                                 >  2:222710/1‑143 (MQ=255)
                                          aCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGa                                                                                            <  4:501897/148‑1 (MQ=255)
                                             cATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                          >  3:968352/1‑115 (MQ=255)
                                             cATTGATCTGACTTTGATTCATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                          <  4:968352/115‑1 (MQ=255)
                                               ttGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                                                                       <  3:800180/132‑1 (MQ=255)
                                               ttGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                                                                       >  4:800180/1‑132 (MQ=255)
                                                   tCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                                                                    <  4:254212/99‑1 (MQ=255)
                                                   tCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                                                                    >  3:254212/1‑99 (MQ=255)
                                                          ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         <  4:287645/119‑1 (MQ=255)
                                                          ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         >  3:287645/1‑119 (MQ=255)
                                                          ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                  <  2:595040/126‑1 (MQ=255)
                                                          ttGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                  >  1:595040/1‑126 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGcc                                                                                                                                <  1:378911/91‑1 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGcc                                                                                                                                >  2:580022/1‑91 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGcc                                                                                                                                <  1:580022/91‑1 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGcc                                                                                                                                >  2:378911/1‑91 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                      >  1:753864/1‑85 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                      <  2:753864/85‑1 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                                                                <  2:448602/91‑1 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGcc                                                                                                                                >  1:448602/1‑91 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              >  2:603875/1‑109 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              <  1:603875/109‑1 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                                                           >  3:432469/1‑144 (MQ=255)
                                                               ttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAcc                                                                           <  4:432469/144‑1 (MQ=255)
                                                                     tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                          <  2:827335/91‑1 (MQ=255)
                                                                     tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                          >  1:827335/1‑91 (MQ=255)
                                                                      cTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                                                                  <  2:638075/82‑1 (MQ=255)
                                                                      cTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                                                                  >  1:638075/1‑82 (MQ=255)
                                                                         gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         <  4:413580/104‑1 (MQ=255)
                                                                         gAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         >  3:413580/1‑104 (MQ=255)
                                                                               aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                    <  2:732948/103‑1 (MQ=255)
                                                                               aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                    >  1:732948/1‑103 (MQ=255)
                                                                               aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                                  >  4:178972/1‑121 (MQ=255)
                                                                               aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                                  <  3:178972/121‑1 (MQ=255)
                                                                                cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACg                                                                                          <  1:102218/112‑1 (MQ=255)
                                                                                cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACg                                                                                          >  2:102218/1‑112 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGc                                                                                                                                 <  2:503579/71‑1 (MQ=38)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGc                                                                                                                                 >  1:503579/1‑71 (MQ=38)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              >  4:441273/1‑90 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              <  3:441273/90‑1 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              >  4:994839/1‑90 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              <  3:994839/90‑1 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                          >  1:1099380/1‑94 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                          <  2:1099380/94‑1 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                      >  2:533622/1‑98 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                      <  1:533622/98‑1 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                                                            <  2:522098/124‑1 (MQ=255)
                                                                                  cGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTAc                                                                            >  1:522098/1‑124 (MQ=255)
                                                                                      aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                      <  3:660786/62‑1 (MQ=255)
                                                                                      aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGa                                                                                                                                      >  4:660786/1‑62 (MQ=255)
                                                                                      aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                    <  1:618471/96‑1 (MQ=255)
                                                                                      aaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGt                                                                                                    >  2:618471/1‑96 (MQ=255)
                                                                                              tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                                                         >  4:1107523/1‑67 (MQ=255)
                                                                                              tGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAt                                                                                                                         <  3:1107523/67‑1 (MQ=255)
                                                                                               gcgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                    <  4:265476/71‑1 (MQ=255)
                                                                                               gcgcAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                    >  3:265476/1‑71 (MQ=255)
                                                                                                  cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                           <  2:444440/77‑1 (MQ=255)
                                                                                                  cAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                           >  1:444440/1‑77 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTg                                                                                                                                  >  4:905624/1‑52 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         <  2:138141/77‑1 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         >  1:138141/1‑77 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt                                                                                 >  2:569476/1‑101 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAAt                                                                                 <  1:569476/101‑1 (MQ=255)
                                                                                                    aCGCGGCAACGGTGTGGAGAAGGGATAAAAAAAAGGGCAAGTCAGTGACCTg                                                                                                                                  <  3:905624/52‑1 (MQ=255)
                                                                                                            aCGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                                                                <  3:1003963/110‑1 (MQ=255)
                                                                                                            aCGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                                                                >  4:1003963/1‑110 (MQ=255)
                                                                                                                               aaaaaaCGGGCAAGTCAGTGACGTGCCCGTTAATTTTCAGAGAAGGGGaa                                                                                                         <  3:274012/50‑1 (MQ=255)
                                                                                                                               aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              <  3:375365/45‑1 (MQ=255)
                                                                                                                               aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAg                                                                                                              >  4:375365/1‑45 (MQ=255)
                                                                                                                               aaaaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                         >  4:274012/1‑50 (MQ=255)
                                                                                                                                atcaaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgt                            >  3:989248/4‑126 (MQ=255)
                                                                                                                                atcaaCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgt                            <  4:989248/123‑1 (MQ=255)
                                                                                                                                     cGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                                                  >  4:840701/1‑83 (MQ=255)
                                                                                                                                     cGGGCAAGTCAGTGACCTGCCCGGTGATTTTCAGAGAAGGGGAATGAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                                                  <  3:840701/83‑1 (MQ=255)
                                                                                                                                         cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATaa  <  4:362577/143‑1 (MQ=255)
                                                                                                                                         cAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATaa  >  3:362577/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAA  >  NC_000913/3057001‑3057280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑