Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 304,088 G→T 6.2% T32N (ACT→AAT)  ykgJ ← UPF0153 cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913304,0880GT 6.2% 218.6 / 3.2 63T32N (ACT→AAT) ykgJUPF0153 cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base T (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAAC  >  NC_000913/303967‑304217
                                                                                                                         |                                                                                                                                 
cGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGcc                                                                                                         >  3:289681/1‑148 (MQ=255)
      gcagGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCg                                                                                                                              >  3:1113754/1‑121 (MQ=255)
      gcagGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCg                                                                                                                              <  4:1113754/121‑1 (MQ=255)
             tttttGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGTACTATTCCCGcc                                                                                                                            >  2:759509/1‑116 (MQ=255)
             tttttGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGTACTATTCCCGcc                                                                                                                            <  1:759509/116‑1 (MQ=255)
                          ttCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGc                                                                               <  4:289681/148‑1 (MQ=255)
                                     gCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcac                                                                    >  4:774939/1‑148 (MQ=255)
                                             tCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTc                                                            >  4:515248/1‑148 (MQ=255)
                                             tCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTc                                                            >  3:782871/1‑148 (MQ=255)
                                                  ggTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgt                                                                                                                    >  1:335133/1‑87 (MQ=255)
                                                  ggTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCAtcgt                                                                                                                    <  2:335133/87‑1 (MQ=255)
                                                  ggTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgca                                                       >  1:1067390/1‑148 (MQ=255)
                                                     ttttCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATgg                                                    <  4:782871/148‑1 (MQ=255)
                                                       ttCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCTCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGAt                                                  <  3:774939/148‑1 (MQ=255)
                                                          tGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCa                                               <  3:515248/148‑1 (MQ=255)
                                                           gATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgcat                                                      <  4:1060983/140‑1 (MQ=255)
                                                           gATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgcat                                                      >  3:1060983/1‑140 (MQ=255)
                                                           gATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAg                                              <  2:1067390/148‑1 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTa                                                                                                                                 >  3:414479/1‑63 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTa                                                                                                                                 <  4:414479/63‑1 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACTGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 >  4:796398/1‑95 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 <  3:796398/95‑1 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgca                                                       <  2:795791/137‑1 (MQ=255)
                                                             ttGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgca                                                       >  1:795791/1‑137 (MQ=255)
                                                                      cTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 >  4:79085/1‑86 (MQ=255)
                                                                      cTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 <  3:79085/86‑1 (MQ=255)
                                                                        cATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGc                                                                      >  3:717926/1‑111 (MQ=255)
                                                                        cATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGc                                                                      <  4:717926/111‑1 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGc                                                                               >  4:341705/1‑100 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGc                                                                               <  3:341705/100‑1 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCacaacat                                                                        >  4:633537/1‑106 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAg                                                                        <  1:143944/107‑1 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAg                                                                        >  2:143944/1‑107 (MQ=255)
                                                                          tGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAg                                                                        <  3:633537/107‑1 (MQ=255)
                                                                            cATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGAcg                                                                                          >  1:705656/1‑87 (MQ=255)
                                                                            cATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGAcg                                                                                          <  2:705656/87‑1 (MQ=255)
                                                                                     aaaGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 <  1:278014/71‑1 (MQ=255)
                                                                                     aaaGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 >  2:278014/1‑71 (MQ=255)
                                                                                        ggggATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACcaca                                                                >  1:349832/1‑101 (MQ=255)
                                                                                        ggggATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACcaca                                                                <  2:349832/101‑1 (MQ=255)
                                                                                             tatTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCg            >  4:354887/1‑148 (MQ=255)
                                                                                                ttGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCGTGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTaa         <  3:777553/148‑1 (MQ=255)
                                                                                                ttGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTaa         >  4:777553/1‑148 (MQ=255)
                                                                                                    tCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 <  1:61391/56‑1 (MQ=255)
                                                                                                    tCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTaaaa                                                                                                 >  2:61391/1‑56 (MQ=255)
                                                                                                    tCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcacc                                                                   <  4:1028389/86‑1 (MQ=255)
                                                                                                    tCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGcacc                                                                   >  3:1028389/1‑86 (MQ=255)
                                                                                                    tCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAagag     >  3:625817/1‑148 (MQ=255)
                                                                                                       gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgc                                                        <  3:750098/94‑1 (MQ=255)
                                                                                                       gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTcatgc                                                        >  4:750098/1‑94 (MQ=255)
                                                                                                       gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATc             <  2:206749/137‑1 (MQ=255)
                                                                                                       gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATc             >  1:206749/1‑137 (MQ=255)
                                                                                                       gTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAAc  <  4:625817/148‑1 (MQ=255)
                                                                                                        tGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCa                            >  2:884788/1‑121 (MQ=255)
                                                                                                        tGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCa                            <  1:884788/121‑1 (MQ=255)
                                                                                                         gAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTc                                                <  4:276901/100‑1 (MQ=255)
                                                                                                         gAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTc                                                >  3:276901/1‑100 (MQ=255)
                                                                                                         gAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCg            <  2:1112982/136‑1 (MQ=255)
                                                                                                         gAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCg            >  1:1112982/1‑136 (MQ=255)
                                                                                                              tGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAag       >  4:1132009/1‑136 (MQ=255)
                                                                                                              tGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAag       <  3:1132009/136‑1 (MQ=255)
                                                                                                               ggCGGTACTATTCCCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTaaaaa               >  3:572727/1‑127 (MQ=255)
                                                                                                               ggCGGTACTATTCCCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTaaaaa               <  4:572727/127‑1 (MQ=255)
                                                                                                                         |                                                                                                                                 
CGTGCAGCAGGCATTTTTGCCCGGGGTTCCTGCAAGGGCAATACATCGGGGGTTTTTCTGATTGGTACCGCTCATGCATCGGTGAAAAGGGGATATTTGTTCAGTGAGCCTGGCGGGAATAGTACCGCCAGCATCGTCGGCTTCGGCCCAGTAAAAAGAGACGCGGAAAAATGCACAACAGGCACCACACGTCATGCATGGATTCAGATTGCTCATAATTCACCAGTACAGCTAAAAATCGTAAAGAGAAC  >  NC_000913/303967‑304217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑