Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,139,922 C→A 5.0% D15Y (GAT→TAT)  hybG ← hydrogenase 2 accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,139,9220CA 5.0% 503.4 / 13.4 139D15Y (GAT→TAT) hybGhydrogenase 2 accessory protein
Reads supporting (aligned to +/- strand):  ref base C (66/66);  new base A (4/3);  total (70/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

Only 100 of 139 total aligned reads displayed.

CTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTG  >  NC_000913/3139778‑3140057
                                                                                                                                                |                                                                                                                                       
cTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTc                                                                                                                                      <  1:176572/148‑1 (MQ=255)
      cgATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGAc                                                                                                                                <  3:967380/148‑1 (MQ=255)
                cATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcc                                                                                                                      >  4:172793/1‑148 (MQ=255)
                     cAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggcc                                                                                                                 >  4:880696/1‑148 (MQ=255)
                        aTCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggcctgg                                                                                                              >  2:259030/1‑148 (MQ=255)
                        aTCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAAGATATTCAc                                                                                                                                    >  2:177433/1‑126 (MQ=255)
                            gACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACg                                                                                                          <  2:107807/148‑1 (MQ=255)
                             aCGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGc                                                                                                         >  1:587685/1‑148 (MQ=255)
                                        cACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACatt                                                                                              <  3:880696/148‑1 (MQ=255)
                                            cACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggcc                                                                                                                 >  2:1015415/1‑125 (MQ=255)
                                            cACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggcc                                                                                                                 <  1:1015415/125‑1 (MQ=255)
                                            cACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcc                                                                                                                      >  1:1126036/1‑120 (MQ=255)
                                            cACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcc                                                                                                                      <  2:1126036/120‑1 (MQ=255)
                                             aCTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTaa                                                                                         >  2:38848/1‑148 (MQ=255)
                                                ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTc                                                                                      <  1:259030/148‑1 (MQ=255)
                                                  cccAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCa                                                                                                       >  2:410825/1‑129 (MQ=255)
                                                    cAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGAcagccagc                                                                                                                         >  2:133162/1‑109 (MQ=255)
                                                    cAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGAcagccagc                                                                                                                         <  1:133162/109‑1 (MQ=255)
                                                    cAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGa                                                                                                             >  4:525827/1‑121 (MQ=255)
                                                    cAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGa                                                                                                             <  3:525827/121‑1 (MQ=255)
                                                     aGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGtt                                                                                 <  3:172793/148‑1 (MQ=255)
                                                     aGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGtt                                                                                 >  2:455871/1‑148 (MQ=255)
                                                      gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTa                                                                                >  4:336956/1‑148 (MQ=255)
                                                       tAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCATCTCCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTAt                                                                               <  2:587685/148‑1 (MQ=255)
                                                         gATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctggc                                                                                                                  >  4:885912/1‑111 (MQ=255)
                                                              gCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCa                                                                            >  4:1018772/1‑144 (MQ=255)
                                                              gCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCa                                                                            <  3:1018772/144‑1 (MQ=255)
                                                               cAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAAGATATTCAcc                                                                                                                                   <  1:61533/88‑1 (MQ=255)
                                                                aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCa                                                                      <  3:336956/148‑1 (MQ=255)
                                                                      aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACtt                                                                <  1:1032650/148‑1 (MQ=255)
                                                                        cTTCACAAATCAGGGCTATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  <  4:139381/112‑1 (MQ=255)
                                                                        cTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  >  3:139381/1‑112 (MQ=255)
                                                                        cTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTAtt                                                                              >  1:270752/1‑132 (MQ=255)
                                                                         ttCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACg                                                                                                          <  1:366213/103‑1 (MQ=255)
                                                                         ttCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACg                                                                                                          >  2:366213/1‑103 (MQ=255)
                                                                          tCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  >  3:767836/1‑110 (MQ=255)
                                                                          tCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  <  4:767836/110‑1 (MQ=255)
                                                                          tCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGa                                                            <  1:455871/148‑1 (MQ=255)
                                                                           cacaAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctg                                                                                                                    >  1:926226/1‑91 (MQ=255)
                                                                           cacaAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCAcctg                                                                                                                    <  2:926226/91‑1 (MQ=255)
                                                                             caAATCAGGGCGATATTCCCATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAAGATATTCAc                                                                                                                                    <  2:965964/73‑1 (MQ=255)
                                                                             caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGt                                                                                  >  2:1118369/1‑123 (MQ=255)
                                                                             caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGt                                                                                  <  1:1118369/123‑1 (MQ=255)
                                                                             caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCa                                                                            <  4:723155/129‑1 (MQ=255)
                                                                             caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAAc                                                                          >  3:1115055/1‑131 (MQ=255)
                                                                             caAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAAc                                                                          <  4:1115055/131‑1 (MQ=255)
                                                                             caAATCAGAGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAAGATATTCAc                                                                                                                                    >  1:965964/1‑73 (MQ=255)
                                                                               aaTCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCg                                                                                    <  3:323117/119‑1 (MQ=255)
                                                                                    gggCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGAt                                                        >  2:536045/1‑142 (MQ=255)
                                                                                    gggCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGAt                                                        <  1:536045/142‑1 (MQ=255)
                                                                                     ggCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGaa                                                 <  1:38848/148‑1 (MQ=255)
                                                                                      gCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAAt                                                <  1:945217/148‑1 (MQ=255)
                                                                                                caTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  >  3:327146/1‑88 (MQ=255)
                                                                                                caTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATaca                                                                                                  <  4:327146/88‑1 (MQ=255)
                                                                                                caTCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTAt                                                                               <  4:211711/107‑1 (MQ=255)
                                                                                                     cgcTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCaa                                                                           >  3:744926/1‑106 (MQ=255)
                                                                                                         tGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACttt                                                               <  2:989146/114‑1 (MQ=255)
                                                                                                         tGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACttt                                                               >  1:989146/1‑114 (MQ=255)
                                                                                                               cacaTACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACt                                                                 >  3:888266/1‑106 (MQ=255)
                                                                                                               cacaTACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGc                                             >  2:1049914/1‑126 (MQ=255)
                                                                                                               cacaTACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGc                                             <  1:1049914/126‑1 (MQ=255)
                                                                                                               cacaTACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTc                       >  2:749523/1‑148 (MQ=255)
                                                                                                                   tACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCTGTTATTCa                                                                            <  4:982189/91‑1 (MQ=255)
                                                                                                                   tACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCa                                                                      <  4:659684/97‑1 (MQ=255)
                                                                                                                   tACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCa                                                                      >  3:659684/1‑97 (MQ=255)
                                                                                                                   tACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATc                                                       <  4:917276/112‑1 (MQ=255)
                                                                                                                   tACTTCAACCAGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATc                                                       >  3:917276/1‑112 (MQ=255)
                                                                                                                      ttCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTAtt                                                                              >  2:927532/1‑86 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGAcag                                                                                                                              <  2:1057553/37‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACg                                                                                                          >  2:345564/1‑57 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCg                                                                                    <  2:992532/79‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCg                                                                                    >  1:992532/1‑79 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCa                                                                            <  1:377683/87‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACtt                                                                <  4:774218/99‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACtt                                                                >  3:774218/1‑99 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             >  3:1048113/1‑102 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             >  1:299678/1‑102 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             <  4:1048113/102‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             <  2:299678/102‑1 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGa                                                            >  2:755734/1‑103 (MQ=255)
                                                                                                                       tCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGa                                                            <  1:755734/103‑1 (MQ=255)
                                                                                                                           ccTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             >  2:907941/1‑98 (MQ=255)
                                                                                                                           ccTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             <  1:907941/98‑1 (MQ=255)
                                                                                                                           ccTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACagag           >  2:633535/1‑148 (MQ=255)
                                                                                                                            cTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGc                                             >  1:317449/1‑113 (MQ=255)
                                                                                                                             tGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             >  4:152800/1‑96 (MQ=255)
                                                                                                                             tGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTg                                                             <  3:152800/96‑1 (MQ=255)
                                                                                                                             tGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCg         >  1:479886/1‑148 (MQ=255)
                                                                                                                              gcgcAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCgg        <  3:991930/148‑1 (MQ=255)
                                                                                                                               cgcAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGa                                                            <  2:711527/95‑1 (MQ=255)
                                                                                                                                 cAAGCTGGTGACTATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATAcaca                                                                                                >  3:80454/1‑57 (MQ=255)
                                                                                                                                 cAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATac                                                                                                   >  1:749763/1‑54 (MQ=255)
                                                                                                                                 cAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATAcaca                                                                                                <  4:80454/57‑1 (MQ=255)
                                                                                                                                    gtcggTGAAGATATTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTg  >  3:1113934/4‑148 (MQ=255)
                                                                                                                                       ggTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGacac                                 <  2:296887/114‑1 (MQ=255)
                                                                                                                                         tGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGaca                                  >  2:859037/1‑111 (MQ=255)
                                                                                                                                         tGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGaca                                  <  1:859037/111‑1 (MQ=255)
                                                                                                                                            atatCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGa                                                            >  1:108106/1‑82 (MQ=255)
                                                                                                                                               tCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCg                                           >  1:27263/1‑96 (MQ=255)
                                                                                                                                               tCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCg                                           <  2:27263/96‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                       
CTTCGTCGATGATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTGAATATCTTCACCGACAGCCAGCACCTGGCCTGGAACGCCAATACACATTATTAACTCCGGTTATTCAACTTCAATACTTTTGACGATCAGCGAATCGCCGGTATCGACACGCAACCGCTCGCCGTGACAGAGCGGACACTG  >  NC_000913/3139778‑3140057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: