| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,187,993 | C→T | 6.8% | pseudogene (567/2439 nt) | yqiG → | pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,187,993 | 0 | C | T | 6.8% | 191.9 / 4.4 | 59 | pseudogene (567/2439 nt) | yqiG | pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (28/27); new base T (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
ATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTT > NC_000913/3187854‑3188138 | aTGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa < 1:348297/145‑1 (MQ=255)aTGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa > 2:348297/1‑145 (MQ=255) taTTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACaa > 1:526869/2‑148 (MQ=255) tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTc > 3:187005/1‑137 (MQ=255) tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTc < 4:187005/137‑1 (MQ=255) tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACACTg < 2:526869/148‑1 (MQ=255) aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgc > 3:323391/1‑120 (MQ=255) aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgc < 4:323391/121‑2 (MQ=255) aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCa > 1:1088244/1‑128 (MQ=255) aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCa < 2:1088244/128‑1 (MQ=255) aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCa > 1:410921/1‑148 (MQ=255) aTTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGa < 3:358161/118‑1 (MQ=255) aTTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGa > 4:358161/1‑118 (MQ=255) tttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCa > 3:443761/1‑111 (MQ=255) tttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCa < 4:443761/111‑1 (MQ=255) ttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTAcc < 3:1074104/109‑1 (MQ=255) ttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTAcc > 4:1074104/1‑109 (MQ=255) ccTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa > 3:814431/1‑106 (MQ=255) ccTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa < 4:814431/106‑1 (MQ=255) tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTa > 4:458748/1‑118 (MQ=255) tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTa < 3:458748/118‑1 (MQ=255) ctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCa > 3:1063524/1‑133 (MQ=255) ctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCa < 4:1063524/133‑1 (MQ=255) cATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAtt < 2:410921/148‑1 (MQ=255) tATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa > 3:913257/1‑98 (MQ=255) tATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTa < 4:913257/98‑1 (MQ=255) aTGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTcc > 1:894146/1‑148 (MQ=255) gggCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa < 2:728261/99‑1 (MQ=255) gggCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa > 1:728261/1‑99 (MQ=255) gCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGt > 2:1090642/1‑90 (MQ=255) gCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGt < 1:1090642/90‑1 (MQ=255) gCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAAt > 2:869317/1‑101 (MQ=255) gCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAAt < 1:869317/101‑1 (MQ=255) cGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACaa < 3:354988/99‑1 (MQ=255) cGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACaa > 4:354988/1‑99 (MQ=255) aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGt < 1:484815/78‑1 (MQ=255) aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGt > 2:484815/1‑78 (MQ=255) tCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCaa > 3:122460/1‑148 (MQ=255) aaaaTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATccc < 2:894146/148‑1 (MQ=255) ttCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACaa > 1:737588/1‑78 (MQ=255) ttCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACaa < 2:737588/78‑1 (MQ=255) tAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg < 3:7261/70‑1 (MQ=255) tAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg > 4:7261/1‑70 (MQ=255) tAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGt < 1:427768/104‑1 (MQ=255) tAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGt > 2:427768/1‑104 (MQ=255) ttGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTgg > 3:280706/1‑78 (MQ=255) ttGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTgg < 4:280706/78‑1 (MQ=255) tGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatga < 4:549479/119‑1 (MQ=255) tGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatga > 3:549479/1‑119 (MQ=255) tGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc < 4:548871/97‑1 (MQ=255) tGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc > 3:548871/1‑97 (MQ=255) tGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAt < 4:122460/148‑1 (MQ=255) cGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg > 4:981875/1‑48 (MQ=37) cGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg < 3:981875/48‑1 (MQ=37) tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatga > 3:115594/1‑101 (MQ=255) tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatga < 4:115594/101‑1 (MQ=255) aCTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaat < 4:604716/102‑1 (MQ=255) aCTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaat > 3:604716/1‑102 (MQ=255) gATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaa > 4:591353/1‑98 (MQ=255) gATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaa < 3:591353/98‑1 (MQ=255) tACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCtt > 3:160345/1‑148 (MQ=255) | ATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTT > NC_000913/3187854‑3188138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |