Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,176 C→A 9.3% A27A (GCG→GCT ttdR ← transcriptional activator of ttdABT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,1760CA 9.3% 146.5 / 5.9 43A27A (GCG→GCTttdRtranscriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base A (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

ACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCC  >  NC_000913/3206053‑3206314
                                                                                                                           |                                                                                                                                          
acGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCtg                                                                                                                    >  1:362798/1‑148 (MQ=255)
             tcagtcagCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTa                                                                                                       <  2:362798/148‑1 (MQ=255)
                                            tCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCt                                                                                                                                 <  4:11356/91‑1 (MQ=255)
                                            tCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGACGCt                                                                                                                                 >  3:11356/1‑91 (MQ=255)
                                               gcagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCt                                                                     >  4:200288/1‑148 (MQ=255)
                                                cagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTa                                                                                                       <  1:545317/113‑1 (MQ=255)
                                                cagcGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTa                                                                                                       >  2:545317/1‑113 (MQ=255)
                                                      tgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                             >  1:5146/1‑133 (MQ=255)
                                                      tgttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                             <  2:5146/133‑1 (MQ=255)
                                                        ttgCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCAt                                                            <  3:200288/148‑1 (MQ=255)
                                                              gCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGacgc                                                                                                                                  >  1:432555/1‑72 (MQ=255)
                                                              gCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGCCGCAGCGGCGacgc                                                                                                                                  <  2:432555/72‑1 (MQ=255)
                                                                    tttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTc                                                                                       <  3:692233/109‑1 (MQ=255)
                                                                    tttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTc                                                                                       >  4:692233/1‑109 (MQ=255)
                                                                    tttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     >  3:420315/1‑111 (MQ=255)
                                                                    tttCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     <  4:420315/111‑1 (MQ=255)
                                                                        aaGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTaa                                                      <  3:762232/138‑1 (MQ=255)
                                                                        aaGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTaa                                                      >  4:762232/1‑138 (MQ=255)
                                                                                 gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                                                                                              <  2:997444/73‑1 (MQ=255)
                                                                                 gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                                                                                              <  2:542637/73‑1 (MQ=255)
                                                                                 gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                                                                                              >  1:997444/1‑73 (MQ=255)
                                                                                 gATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACg                                                                                                              >  1:542637/1‑73 (MQ=255)
                                                                                   tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                           >  4:593123/1‑138 (MQ=255)
                                                                                   tCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGt                                           <  3:593123/138‑1 (MQ=255)
                                                                                    ccGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     >  3:789575/1‑95 (MQ=255)
                                                                                    ccGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     <  4:789575/95‑1 (MQ=255)
                                                                                       cTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGc                                                                          <  4:1113097/103‑1 (MQ=255)
                                                                                       cTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGc                                                                          >  3:1113097/1‑103 (MQ=255)
                                                                                         tgatGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTa                                                                                                       >  4:721213/4‑72 (MQ=255)
                                                                                         tGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTa                                                                                                       <  3:721213/72‑1 (MQ=255)
                                                                                                  aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                             <  3:586742/89‑1 (MQ=255)
                                                                                                  aTGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTaaa                                                                             >  4:586742/1‑89 (MQ=255)
                                                                                                       ggCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                 <  3:667655/96‑1 (MQ=255)
                                                                                                       ggCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                 >  4:667655/1‑96 (MQ=255)
                                                                                                               cccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     >  1:129448/1‑68 (MQ=255)
                                                                                                               cccAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCtt                                                                                     <  2:129448/68‑1 (MQ=255)
                                                                                                                 cAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAgg                                                                           <  4:749122/76‑1 (MQ=255)
                                                                                                                 cAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAgg                                                                           >  3:749122/1‑76 (MQ=255)
                                                                                                                 cAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                 <  3:338200/86‑1 (MQ=255)
                                                                                                                 cAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTc                                                                 >  4:338200/1‑86 (MQ=255)
                                                                                                                  aaGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgtg                                       <  4:437029/111‑1 (MQ=255)
                                                                                                                  aaGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTtgtg                                       >  3:437029/1‑111 (MQ=255)
                                                                                                                  aaGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTccc  >  3:237235/1‑148 (MQ=255)
                                                                                                                           |                                                                                                                                          
ACGTTGGCCACTTTCAGTCAGCGCCACGCCGCGAGCGGAGCGGTTCAGCAGCGTTGTTGCCAGCGTATTTTCAAGGATTTGGATCCGCTTGGTGACAAATGCAGGCGTCTGCCCAAGCGTCGCCGCTGCGGCGCTAAAACTCCCGCTGTGAACGATTTCTACCAGTACCTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCC  >  NC_000913/3206053‑3206314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: