| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,221,151 | T→C | 5.8% | I33V (ATC→GTC) ‡ | ygjH ← | putative tRNA binding protein; putative tRNA corner chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,221,151 | 0 | T | C | 5.8% | 228.8 / 3.8 | 69 | I33V (ATC→GTC) ‡ | ygjH | putative tRNA binding protein; putative tRNA corner chaperone |
| Reads supporting (aligned to +/- strand): ref base T (31/34); new base C (2/2); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
TTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTT > NC_000913/3221008‑3221260 | ttCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTAc > 2:1068144/1‑148 (MQ=255) ccGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAg < 4:411681/148‑1 (MQ=255) cGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTg < 1:1068144/148‑1 (MQ=255) cGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt > 3:414353/1‑148 (MQ=255) cGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt < 2:37405/148‑1 (MQ=255) tCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCg < 4:414353/148‑1 (MQ=255) tttttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGt > 2:71198/1‑119 (MQ=255) tttttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGt < 1:71198/119‑1 (MQ=255) cAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTcc > 1:666497/1‑148 (MQ=255) aTTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc > 1:169987/1‑129 (MQ=255) aTTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc < 2:657777/129‑1 (MQ=255) aTTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc < 2:169987/129‑1 (MQ=255) aTTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc > 1:657777/1‑129 (MQ=255) tAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTGCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc < 2:321011/138‑1 (MQ=255) tAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc > 1:321011/1‑138 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGttt > 3:1029455/1‑114 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGttt < 4:1029455/114‑1 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc < 3:317946/131‑1 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc > 4:317946/1‑131 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCttt < 3:19720/141‑1 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCttt > 4:19720/1‑141 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACATCGTACAgg < 4:824883/102‑2 (MQ=255) accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACATCGTACAgg > 3:824883/1‑101 (MQ=255) ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc < 2:20860/130‑1 (MQ=255) ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc > 1:20860/1‑130 (MQ=255) caccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTTTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACgcgc > 1:622589/1‑148 (MQ=255) ccccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGa < 2:666497/148‑1 (MQ=255) cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt < 4:626129/103‑1 (MQ=255) cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt > 3:626129/1‑103 (MQ=255) ctctTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcg < 2:605586/127‑1 (MQ=255) ctctTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcg > 1:605586/1‑127 (MQ=255) tcttctTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATgg < 4:316891/99‑1 (MQ=255) tcttctTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATgg > 3:316891/1‑99 (MQ=255) cttcttCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCaaaa < 4:758771/146‑1 (MQ=255) cttcttCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCaaaa > 3:758771/1‑146 (MQ=255) tcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGTCGTACAGGTTga < 2:774817/79‑2 (MQ=255) tcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGTCGTACAGGTTga > 1:774817/1‑78 (MQ=255) tcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc < 4:1131339/89‑1 (MQ=255) tcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTc > 3:1131339/1‑89 (MQ=255) cGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTa < 2:622589/148‑1 (MQ=255) agtaCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt < 4:1093351/81‑1 (MQ=255) agtaCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt > 3:1093351/1‑81 (MQ=255) taCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACg < 2:509556/125‑1 (MQ=255) taCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACg > 1:509556/1‑125 (MQ=255) ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTc > 3:254234/1‑109 (MQ=255) ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTc < 4:254234/109‑1 (MQ=255) ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCa > 4:828027/1‑125 (MQ=255) ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCa < 3:828027/125‑1 (MQ=255) cTGGTCCCGCTTTGCAGCCTTTTTTGTCCCCCATCAGCCTGTACGATGTACAGCTGGTCGGAGTCTTCATGGCGTTTCACTTCAACAGTCTTTCAGCCGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACCGTTTCCATTGCtt < 3:993223/148‑1 (MQ=255) cACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCa > 1:106426/1‑64 (MQ=255) cACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCa < 2:106426/64‑1 (MQ=255) tttGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGTGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGc > 3:208974/1‑115 (MQ=255) tttGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGTGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGc < 4:208974/115‑1 (MQ=255) gCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt < 2:896141/76‑1 (MQ=255) gCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt > 1:896141/1‑76 (MQ=255) cccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAc < 4:548615/71‑1 (MQ=255) cccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAc > 3:548615/1‑71 (MQ=255) ccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg > 3:533842/1‑96 (MQ=255) ccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg < 4:533842/96‑1 (MQ=255) cacaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg < 3:938488/95‑1 (MQ=255) cacaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg > 4:938488/1‑95 (MQ=255) cacaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACg < 2:564976/106‑1 (MQ=255) cacaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACg > 1:564976/1‑106 (MQ=255) tCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTg > 2:196014/1‑82 (MQ=255) tCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTg < 1:196014/82‑1 (MQ=255) tCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt < 1:739684/115‑1 (MQ=255) tCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt > 2:739684/1‑115 (MQ=255) tGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCtt < 4:122944/117‑1 (MQ=255) tGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCtt > 3:122944/1‑117 (MQ=255) | TTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTT > NC_000913/3221008‑3221260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |