| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,271,825 | T→A | 11.6% | intergenic (‑55/+42) | garK ← / ← garR | glycerate kinase I/tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,271,825 | 0 | T | A | 11.6% | 106.0 / 3.4 | 34 | intergenic (‑55/+42) | garK/garR | glycerate kinase I/tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base A (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAG > NC_000913/3271688‑3271953 | cGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTc > 1:500191/1‑140 (MQ=255)cGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTc < 2:500191/140‑1 (MQ=255)cGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGcc < 4:1136177/146‑1 (MQ=255)cGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGcc > 3:1136177/1‑146 (MQ=255) agagTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACg < 1:1079279/129‑1 (MQ=255) agagTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACg > 2:1079279/1‑129 (MQ=255) aCGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTaa < 2:661794/111‑1 (MQ=255) aCGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTaa > 1:661794/1‑111 (MQ=255) tACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGc > 1:500928/1‑72 (MQ=255) tACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGc < 2:500928/72‑1 (MQ=255) aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAg > 2:718597/1‑104 (MQ=255) aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAg < 1:718597/104‑1 (MQ=255) aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgt < 3:1065972/131‑1 (MQ=255) aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgt > 4:1065972/1‑131 (MQ=255) tGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATcc > 1:10497/1‑148 (MQ=255) ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt > 3:202016/1‑148 (MQ=255) aCGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACttt > 3:425808/1‑96 (MQ=255) aCGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACttt < 4:425808/96‑1 (MQ=255) gCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAcc < 2:10497/148‑1 (MQ=255) ccGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa < 4:202016/148‑1 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCg < 2:658525/73‑1 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCg > 1:658525/1‑73 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg > 3:493578/1‑109 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcg < 4:493578/109‑1 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAg < 2:802035/144‑1 (MQ=255) ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAg > 1:802035/1‑144 (MQ=255) tATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa < 2:158167/131‑1 (MQ=255) tATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCa > 1:158167/1‑131 (MQ=255) ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACtt < 2:419553/73‑1 (MQ=255) ttCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACtt > 1:419553/1‑73 (MQ=255) ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgt < 2:759525/85‑1 (MQ=255) ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgt > 1:759525/1‑85 (MQ=255) ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt < 3:18476/116‑1 (MQ=255) ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt > 4:18476/1‑116 (MQ=255) | CGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAG > NC_000913/3271688‑3271953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |