Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,380,440 C→A 6.9% L84M (CTG→ATG) ‡ yhcB → DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,380,4400CA 6.9% 414.9 / 15.8 116L84M (CTG→ATG) ‡yhcBDUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand):  ref base C (53/55);  new base A (4/4);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 117 total aligned reads displayed.

ACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGA  >  NC_000913/3380297‑3380578
                                                                                                                                               |                                                                                                                                          
aCGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgct                                                                                                                                        >  4:142786/1‑148 (MQ=255)
           aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   >  1:9240/1‑142 (MQ=255)
           aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGt                                                                                                                             >  2:678846/1‑148 (MQ=255)
                tAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   >  4:61947/1‑137 (MQ=255)
                tAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   <  3:61947/137‑1 (MQ=255)
                  aaGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      <  3:142786/148‑1 (MQ=255)
                        aaCTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                >  4:1065348/1‑148 (MQ=255)
                        aaCTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                >  2:808270/1‑148 (MQ=255)
                             gACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGt                                                                                                           <  1:678846/148‑1 (MQ=255)
                              aCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGt                                                                                                                             >  1:511071/1‑129 (MQ=255)
                              aCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGt                                                                                                                             <  2:511071/129‑1 (MQ=255)
                                   tATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     <  1:808270/148‑1 (MQ=255)
                                   tATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     <  3:1065348/148‑1 (MQ=255)
                                      cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                >  3:1003406/1‑134 (MQ=255)
                                      cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                <  4:1003406/134‑1 (MQ=255)
                                      cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                <  2:725690/134‑1 (MQ=255)
                                      cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                >  1:725690/1‑134 (MQ=255)
                                           agagCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      >  2:907544/1‑123 (MQ=255)
                                           agagCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      <  1:907544/123‑1 (MQ=255)
                                             agCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGc                                                                                           >  3:802322/1‑148 (MQ=255)
                                                   ttAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                    >  2:805214/1‑101 (MQ=255)
                                                   ttAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                    <  1:805214/101‑1 (MQ=255)
                                                         aCTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGa                                                                                                                                  >  1:292773/1‑97 (MQ=255)
                                                         aCTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGa                                                                                                                                  <  2:292773/97‑1 (MQ=255)
                                                          cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           <  3:501075/103‑1 (MQ=255)
                                                          cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           >  4:501075/1‑103 (MQ=255)
                                                          cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                          <  3:1060279/120‑1 (MQ=255)
                                                          cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                          >  4:1060279/1‑120 (MQ=255)
                                                                 cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     <  1:74747/118‑1 (MQ=255)
                                                                 cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     >  2:74747/1‑118 (MQ=255)
                                                                 cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACg                                                                       >  3:277753/1‑148 (MQ=255)
                                                                 cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACg                                                                       >  3:22315/1‑148 (MQ=255)
                                                                 cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACg                                                                       <  4:22315/148‑1 (MQ=255)
                                                                  gCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCa                                                                             >  3:1135252/1‑141 (MQ=255)
                                                                  gCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCa                                                                             <  4:226278/141‑1 (MQ=255)
                                                                   cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCtt                                                                                                                              >  1:1093391/1‑90 (MQ=38)
                                                                   cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCtt                                                                                                                              <  2:1093391/91‑2 (MQ=38)
                                                                        cgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                >  4:1023235/1‑148 (MQ=255)
                                                                                 tGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   >  2:633300/1‑136 (MQ=255)
                                                                                 tGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   <  1:633300/136‑1 (MQ=255)
                                                                                     tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGaa                                                                                                                     <  2:189083/82‑1 (MQ=255)
                                                                                     tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGaa                                                                                                                     >  1:189083/1‑82 (MQ=255)
                                                                                     atctaTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATg                                                   <  3:502443/144‑1 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     >  4:591820/1‑94 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  2:279895/113‑1 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  >  1:279895/1‑113 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCg                                                            <  1:1073576/135‑1 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCg                                                            >  2:1073576/1‑135 (MQ=255)
                                                                                         aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                               >  4:286056/1‑148 (MQ=255)
                                                                                          tGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcg                                              <  3:286056/148‑1 (MQ=255)
                                                                                            gcgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   <  4:1034762/125‑1 (MQ=255)
                                                                                               cACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACt                                         >  2:106461/1‑148 (MQ=255)
                                                                                                  gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  4:802322/148‑1 (MQ=255)
                                                                                                  gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  3:1023235/148‑1 (MQ=255)
                                                                                                   aCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               <  2:586736/106‑1 (MQ=255)
                                                                                                   aCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               >  1:586736/1‑106 (MQ=255)
                                                                                                     tATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  2:289553/130‑1 (MQ=255)
                                                                                                     tATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  1:289553/1‑130 (MQ=255)
                                                                                                      aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAg                                                      >  3:597484/1‑128 (MQ=255)
                                                                                                      aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAg                                                      <  4:597484/128‑1 (MQ=255)
                                                                                                      aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  <  4:277753/148‑1 (MQ=255)
                                                                                                      aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  >  2:625503/1‑148 (MQ=255)
                                                                                                       tCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGaa                                 <  1:106461/148‑1 (MQ=255)
                                                                                                          ccAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgc                              <  1:625503/148‑1 (MQ=255)
                                                                                                           cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     <  1:800240/108‑1 (MQ=255)
                                                                                                           cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     <  1:762709/108‑1 (MQ=255)
                                                                                                           cAGCTCTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     >  2:800240/1‑108 (MQ=255)
                                                                                                                  aTCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           <  3:1027244/47‑1 (MQ=255)
                                                                                                                  aTCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           >  4:1027244/1‑47 (MQ=255)
                                                                                                                    cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCGGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               >  2:1109389/1‑89 (MQ=255)
                                                                                                                    cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCGGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               <  1:1109389/89‑1 (MQ=255)
                                                                                                                    cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      >  2:306397/1‑130 (MQ=255)
                                                                                                                    cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  1:306397/130‑1 (MQ=255)
                                                                                                                       cacaTGGCAAGAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGt                                                                                      >  1:592866/1‑79 (MQ=255)
                                                                                                                       cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGt                                                                                      <  2:592866/79‑1 (MQ=255)
                                                                                                                       cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   >  2:538996/1‑98 (MQ=255)
                                                                                                                       cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   <  1:538996/98‑1 (MQ=255)
                                                                                                                           tGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  3:1016620/79‑1 (MQ=255)
                                                                                                                             gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAAt                                                                                                   >  3:1002349/1‑60 (MQ=255)
                                                                                                                             gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAAt                                                                                                   <  4:1002349/60‑1 (MQ=255)
                                                                                                                               aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  2:868083/75‑1 (MQ=255)
                                                                                                                               aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  >  1:868083/1‑75 (MQ=255)
                                                                                                                                aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                              >  1:7306/1‑78 (MQ=255)
                                                                                                                                aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctg                  <  1:551659/138‑1 (MQ=255)
                                                                                                                                aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctg                  >  2:551659/1‑138 (MQ=255)
                                                                                                                                aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCGGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                              <  2:7306/78‑1 (MQ=255)
                                                                                                                                     cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  3:42275/1‑98 (MQ=255)
                                                                                                                                     cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  4:42275/98‑1 (MQ=255)
                                                                                                                                      tCCGGCAGCAGGCTGCTGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  >  3:965265/1‑68 (MQ=38)
                                                                                                                                      tCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  4:1085972/97‑1 (MQ=255)
                                                                                                                                      tCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  3:1085972/1‑97 (MQ=255)
                                                                                                                                        cagcagCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAg                                <  3:469219/116‑1 (MQ=255)
                                                                                                                                         agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcgcg                                            >  1:370317/1‑103 (MQ=255)
                                                                                                                                         agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcgcg                                            <  2:370317/103‑1 (MQ=255)
                                                                                                                                         agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCt                                    <  2:942190/111‑1 (MQ=255)
                                                                                                                                         agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa  >  2:345440/1‑145 (MQ=255)
                                                                                                                                         agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa  <  1:345440/145‑1 (MQ=255)
                                                                                                                                          gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAgg                               >  1:706970/1‑115 (MQ=255)
                                                                                                                                          gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAgg                               <  2:706970/115‑1 (MQ=255)
                                                                                                                                             gcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  <  2:798160/109‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
ACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGA  >  NC_000913/3380297‑3380578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: